Development of a Cell-Culture-Based Platform for Viral Vaccine Production View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2010-06-02

AUTHORS

G. Forno , C. Paillet , R. Kratje , M. Etcheverrigaray

ABSTRACT

Cell-culture-based processes are a suitable alternative for viral vaccine production since they provide several advantages over other manufacturing methods. Since Vero cells are currently considered an acceptable cell substrate to express a variety of viruses, we developed a virus production platform using Vero cells adapted to grow in suspension in a serum free medium. After adapting anchorage dependent Vero cells to grow as a free-cell suspension, the cell line was capable of proliferating in a chemically defined synthetic medium. The production yields of vesicular stomatitis virus, herpes simplex virus 1 and polio virus 1 were evaluated using batch cultures of adherent Vero cells growing in T-flasks and the cells growing in suspension in spinner flasks. The effects of the multiplicity of infection (m.o.i), harvesting time, cell concentration and use of serum were evaluated. Under optimized conditions, similar production yields were achieved with both systems. Perfusion cultures in a bioreactor reached a cell density of 1 × 107 cells/ml. Infection with vesicular stomatitis virus at m.o.i = 0.02 resulted in a viral titer of 2 × 1010 TCID50/ml at 25 h post infection. The viral specific production was 4 times higher than the obtained in batch experiments. These results constitute valuable information for the development of a low-cost large-scale process using a suspension culture of Vero cells for producing viral vaccines. More... »

PAGES

481-484

Book

TITLE

Cells and Culture

ISBN

978-90-481-3418-2
978-90-481-3419-9

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-90-481-3419-9_85

DOI

http://dx.doi.org/10.1007/978-90-481-3419-9_85

DIMENSIONS

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