Nanotechnology in Mechanobiology: Mechanical Manipulation of Cells and Organelle While Monitoring Intracellular Signaling View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2011

AUTHORS

Hitoshi Tatsumi , Kimihide Hayakawa , Masahiro Sokabe

ABSTRACT

Cell migration requires a regulated interplay of actin-filament dynamics and turnover of cell-matrix adhesions. These processes are mechanically coupled by a large complex of cytoplasmic proteins linking adhesive molecules to cytoskeletons. To explore the molecular mechanisms underlying the force-dependent cell responses including remodeling of cytoskeletons, focal adhesive contacts, and of cell-shapes, it is crucial to apply precisely controlled mechanical stimuli (ranging from pN to nN) to cells and to observe the response with a high spatial–temporal resolution (ranging from 100 nm to 1 μm, and 1 ms to 1 min) in living cells. In this chapter, we describe a variety of methods for controlled mechanical stimulations and for monitoring the responses of cells, and our recent research. We employed precisely controlled mechanical stimuli to explore the molecular mechanism underlying the mechanosensing, and the mechano-induced signaling, e.g., activation of mechanosensitive (MS) channels, intracellular calcium ion concentration ([Ca2+]i) increases, adhesive contact formation and mechano-induced reorganization of cytoskeletons in live cells including neuronal growth cones and human umbilical vein endothelial cells (HUVECs). More... »

PAGES

3-19

Book

TITLE

Mechanosensing Biology

ISBN

978-4-431-89756-9
978-4-431-89757-6

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-4-431-89757-6_1

DOI

http://dx.doi.org/10.1007/978-4-431-89757-6_1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028070481


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