Ontology type: schema:Chapter
1998
AUTHORSJae Hee Shim , Yong Doo Park , Won Hee Choi , Jongwon Kim , Seonyang Park , Byoung Goo Min
ABSTRACTLumbrokinase (LK), a trypsin-like enzyme, was efficiently purified from a crude preparation of earthworm (Lumbricus rubellus). The purification procedure was as follows: dissolving crude powder in saline for 3 days; 30%-60% ammonium sulfate gradient fractionation of the soluble fraction; and column chromatography on DEAE-cellulose anion exchange and then p-aminobenzamidine sepharose 6B. Lumbrokinase has a molecular weight of 34.2 kDa, is not dependent on metal ions, is heat-stable, and has a very broad optimal pH range. To examine whether LK hydrolyzed fibrin directly without human blood cell damage, various human blood cells were prepared for characterization of LK activity. The hemolysis of erythrocytes was assayed using a UV spectrophotometer, the degradation pattern of plasma proteins was assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the lysis of protein constituents of platelet-rich plasma and platelets (platelets were separated by Bio-gel A-50 gel filtration) was measured. To investigate whether LK causes spontaneous platelet aggregation, the effect of antiaggregation by adenosine diphosphate (ADP) was measured using an aggregometer. It was shown that, in spite of its proteolytic activity, LK did not cause lysis of erythrocytes, or excessive degradation of protein in plasma, platelets, or platelet-rich plasma. Nor did LK cause spontaneous platelet aggregation. The antiaggregation effect by ADP was not observed. More... »
PAGES471-475
Heart Replacement
ISBN
978-4-431-65923-5
978-4-431-65921-1
http://scigraph.springernature.com/pub.10.1007/978-4-431-65921-1_75
DOIhttp://dx.doi.org/10.1007/978-4-431-65921-1_75
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1041802829
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Seoul National University",
"id": "https://www.grid.ac/institutes/grid.31501.36",
"name": [
"Institute of Biomedical Engineering, College of Medicine, Seoul National University, 28 Youngun-Dong, Chongno-Ku, Seoul, 110-744, Korea"
],
"type": "Organization"
},
"familyName": "Shim",
"givenName": "Jae Hee",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Seoul National University",
"id": "https://www.grid.ac/institutes/grid.31501.36",
"name": [
"Institute of Biomedical Engineering, College of Medicine, Seoul National University, 28 Youngun-Dong, Chongno-Ku, Seoul, 110-744, Korea"
],
"type": "Organization"
},
"familyName": "Park",
"givenName": "Yong Doo",
"id": "sg:person.0732400355.07",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732400355.07"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Seoul National University",
"id": "https://www.grid.ac/institutes/grid.31501.36",
"name": [
"Institute of Biomedical Engineering, College of Medicine, Seoul National University, 28 Youngun-Dong, Chongno-Ku, Seoul, 110-744, Korea"
],
"type": "Organization"
},
"familyName": "Choi",
"givenName": "Won Hee",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Seoul National University",
"id": "https://www.grid.ac/institutes/grid.31501.36",
"name": [
"Institute of Biomedical Engineering, College of Medicine, Seoul National University, 28 Youngun-Dong, Chongno-Ku, Seoul, 110-744, Korea"
],
"type": "Organization"
},
"familyName": "Kim",
"givenName": "Jongwon",
"id": "sg:person.013611426734.26",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013611426734.26"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Seoul National University",
"id": "https://www.grid.ac/institutes/grid.31501.36",
"name": [
"Department of Internal Medicine, College of Medicine, Seoul National University, Seoul, Korea"
],
"type": "Organization"
},
"familyName": "Park",
"givenName": "Seonyang",
"id": "sg:person.01040743514.14",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01040743514.14"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Seoul National University",
"id": "https://www.grid.ac/institutes/grid.31501.36",
"name": [
"Institute of Biomedical Engineering, College of Medicine, Seoul National University, 28 Youngun-Dong, Chongno-Ku, Seoul, 110-744, Korea"
],
"type": "Organization"
},
"familyName": "Min",
"givenName": "Byoung Goo",
"id": "sg:person.0734612500.12",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0734612500.12"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1016/0003-2697(76)90527-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025529346"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0003-9861(52)90121-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041364539"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1159/000216121",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045745469"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2170/jjphysiol.41.461",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051426858"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/00002480-199339030-00033",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060147623"
],
"type": "CreativeWork"
}
],
"datePublished": "1998",
"datePublishedReg": "1998-01-01",
"description": "Lumbrokinase (LK), a trypsin-like enzyme, was efficiently purified from a crude preparation of earthworm (Lumbricus rubellus). The purification procedure was as follows: dissolving crude powder in saline for 3 days; 30%-60% ammonium sulfate gradient fractionation of the soluble fraction; and column chromatography on DEAE-cellulose anion exchange and then p-aminobenzamidine sepharose 6B. Lumbrokinase has a molecular weight of 34.2 kDa, is not dependent on metal ions, is heat-stable, and has a very broad optimal pH range. To examine whether LK hydrolyzed fibrin directly without human blood cell damage, various human blood cells were prepared for characterization of LK activity. The hemolysis of erythrocytes was assayed using a UV spectrophotometer, the degradation pattern of plasma proteins was assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the lysis of protein constituents of platelet-rich plasma and platelets (platelets were separated by Bio-gel A-50 gel filtration) was measured. To investigate whether LK causes spontaneous platelet aggregation, the effect of antiaggregation by adenosine diphosphate (ADP) was measured using an aggregometer. It was shown that, in spite of its proteolytic activity, LK did not cause lysis of erythrocytes, or excessive degradation of protein in plasma, platelets, or platelet-rich plasma. Nor did LK cause spontaneous platelet aggregation. The antiaggregation effect by ADP was not observed.",
"editor": [
{
"familyName": "Akutsu",
"givenName": "Tetsuzo",
"type": "Person"
},
{
"familyName": "Koyanagi",
"givenName": "Hitoshi",
"type": "Person"
}
],
"genre": "chapter",
"id": "sg:pub.10.1007/978-4-431-65921-1_75",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": {
"isbn": [
"978-4-431-65923-5",
"978-4-431-65921-1"
],
"name": "Heart Replacement",
"type": "Book"
},
"name": "The Effect of Lumbrokinase, a Trypsin-Like Enzyme from Lumbricus rubellus, on Human Blood Cells",
"pagination": "471-475",
"productId": [
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/978-4-431-65921-1_75"
]
},
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"c72150a8f59b6e0dcf3391a0a3e19c1ba73bde5e64e4e5a39afb72f23d1ab0c5"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1041802829"
]
}
],
"publisher": {
"location": "Tokyo",
"name": "Springer Japan",
"type": "Organisation"
},
"sameAs": [
"https://doi.org/10.1007/978-4-431-65921-1_75",
"https://app.dimensions.ai/details/publication/pub.1041802829"
],
"sdDataset": "chapters",
"sdDatePublished": "2019-04-15T22:00",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000269.jsonl",
"type": "Chapter",
"url": "http://link.springer.com/10.1007/978-4-431-65921-1_75"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-4-431-65921-1_75'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-4-431-65921-1_75'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-4-431-65921-1_75'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-4-431-65921-1_75'
This table displays all metadata directly associated to this object as RDF triples.
119 TRIPLES
23 PREDICATES
32 URIs
20 LITERALS
8 BLANK NODES