Estimation of Biocompatibility of Polymeric Materials Using RT-PCR Method View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1996

AUTHORS

Shinya Kato , Kaori Ohmura , Akio Kishida , Kazuhisa Sugimura , Mitsuru Akashi

ABSTRACT

One of the most important factors in determining biocompatibility is the cellular response at the tissue-biomaterial interface. In this study, we would introduce a novel research method for the estimation of biocompatibility of polymeric materials, which is the evaluation of mRNA expression of the cells contacting with polymer surfaces using reverse transcription-polymerase chain reaction(RT-PCR) method. Interleukin-1β (IL-1β) were selected to estimation of the extent of inflammation. The various polymer films were put into the 6weli tissue culture plate. Human premyelocytic leukemia cell line(HL-60) were added to each well in containing 10% serum and with or without iipopolysaccharide(LPS) stimulation, or without serum, and were differentiated to macrophage-like cells by phorbol 12-myristate 13-acetate. After predetermined time, the total RNA were isolated from HL-60 cells and mRNA expression were quantitated by RT-PCR method. As a result, in the presence of serum and LPS stimulation, the expression of IL-1β mRNA increased with increasing the contact angle of films and reached to maximum at 70–80°. In the case of without LPS stimulation, IL-1β mRNA expression was decreased compared to the condition of presence of serum and LPS stimulation. From other results, IL-1β mRNA expression was influenced by the kind of substrate, LPS stimulation, serum presence and their combination. We assumed that RT-PCR method is one of the powerful methods to study the biocompatibility of materials. More... »

PAGES

301-302

Book

TITLE

Advanced Biomaterials in Biomedical Engineering and Drug Delivery Systems

ISBN

978-4-431-65885-6
978-4-431-65883-2

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-4-431-65883-2_87

DOI

http://dx.doi.org/10.1007/978-4-431-65883-2_87

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019786174


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0303", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Macromolecular and Materials Chemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Kagoshima University", 
          "id": "https://www.grid.ac/institutes/grid.258333.c", 
          "name": [
            "Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kato", 
        "givenName": "Shinya", 
        "id": "sg:person.01050701273.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050701273.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kagoshima University", 
          "id": "https://www.grid.ac/institutes/grid.258333.c", 
          "name": [
            "Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ohmura", 
        "givenName": "Kaori", 
        "id": "sg:person.012224463163.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012224463163.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kagoshima University", 
          "id": "https://www.grid.ac/institutes/grid.258333.c", 
          "name": [
            "Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kishida", 
        "givenName": "Akio", 
        "id": "sg:person.0714140254.17", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714140254.17"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kagoshima University", 
          "id": "https://www.grid.ac/institutes/grid.258333.c", 
          "name": [
            "Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sugimura", 
        "givenName": "Kazuhisa", 
        "id": "sg:person.010753160655.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010753160655.67"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kagoshima University", 
          "id": "https://www.grid.ac/institutes/grid.258333.c", 
          "name": [
            "Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Akashi", 
        "givenName": "Mitsuru", 
        "id": "sg:person.013674666001.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013674666001.05"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1172/jci116548", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011103415"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(87)90021-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013321498"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jbm.820260702", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027630933"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jbm.820260702", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027630933"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1996", 
    "datePublishedReg": "1996-01-01", 
    "description": "One of the most important factors in determining biocompatibility is the cellular response at the tissue-biomaterial interface. In this study, we would introduce a novel research method for the estimation of biocompatibility of polymeric materials, which is the evaluation of mRNA expression of the cells contacting with polymer surfaces using reverse transcription-polymerase chain reaction(RT-PCR) method. Interleukin-1\u03b2 (IL-1\u03b2) were selected to estimation of the extent of inflammation. The various polymer films were put into the 6weli tissue culture plate. Human premyelocytic leukemia cell line(HL-60) were added to each well in containing 10% serum and with or without iipopolysaccharide(LPS) stimulation, or without serum, and were differentiated to macrophage-like cells by phorbol 12-myristate 13-acetate. After predetermined time, the total RNA were isolated from HL-60 cells and mRNA expression were quantitated by RT-PCR method. As a result, in the presence of serum and LPS stimulation, the expression of IL-1\u03b2 mRNA increased with increasing the contact angle of films and reached to maximum at 70\u201380\u00b0. In the case of without LPS stimulation, IL-1\u03b2 mRNA expression was decreased compared to the condition of presence of serum and LPS stimulation. From other results, IL-1\u03b2 mRNA expression was influenced by the kind of substrate, LPS stimulation, serum presence and their combination. We assumed that RT-PCR method is one of the powerful methods to study the biocompatibility of materials.", 
    "editor": [
      {
        "familyName": "Ogata", 
        "givenName": "Naoya", 
        "type": "Person"
      }, 
      {
        "familyName": "Kim", 
        "givenName": "Sung Wan", 
        "type": "Person"
      }, 
      {
        "familyName": "Feijen", 
        "givenName": "Jan", 
        "type": "Person"
      }, 
      {
        "familyName": "Okano", 
        "givenName": "Teruo", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-4-431-65883-2_87", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-4-431-65885-6", 
        "978-4-431-65883-2"
      ], 
      "name": "Advanced Biomaterials in Biomedical Engineering and Drug Delivery Systems", 
      "type": "Book"
    }, 
    "name": "Estimation of Biocompatibility of Polymeric Materials Using RT-PCR Method", 
    "pagination": "301-302", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-4-431-65883-2_87"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "5e45ab61a567798ecdcfac3c5b5e6a07c6be6a1328e33f02739da75b7a789e07"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1019786174"
        ]
      }
    ], 
    "publisher": {
      "location": "Tokyo", 
      "name": "Springer Japan", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-4-431-65883-2_87", 
      "https://app.dimensions.ai/details/publication/pub.1019786174"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-15T18:10", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8681_00000255.jsonl", 
    "type": "Chapter", 
    "url": "http://link.springer.com/10.1007/978-4-431-65883-2_87"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-4-431-65883-2_87'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-4-431-65883-2_87'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-4-431-65883-2_87'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-4-431-65883-2_87'


 

This table displays all metadata directly associated to this object as RDF triples.

117 TRIPLES      23 PREDICATES      30 URIs      20 LITERALS      8 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-4-431-65883-2_87 schema:about anzsrc-for:03
2 anzsrc-for:0303
3 schema:author N7fb126835fbf4b1e841775708131c6c4
4 schema:citation https://doi.org/10.1002/jbm.820260702
5 https://doi.org/10.1016/0003-2697(87)90021-2
6 https://doi.org/10.1172/jci116548
7 schema:datePublished 1996
8 schema:datePublishedReg 1996-01-01
9 schema:description One of the most important factors in determining biocompatibility is the cellular response at the tissue-biomaterial interface. In this study, we would introduce a novel research method for the estimation of biocompatibility of polymeric materials, which is the evaluation of mRNA expression of the cells contacting with polymer surfaces using reverse transcription-polymerase chain reaction(RT-PCR) method. Interleukin-1β (IL-1β) were selected to estimation of the extent of inflammation. The various polymer films were put into the 6weli tissue culture plate. Human premyelocytic leukemia cell line(HL-60) were added to each well in containing 10% serum and with or without iipopolysaccharide(LPS) stimulation, or without serum, and were differentiated to macrophage-like cells by phorbol 12-myristate 13-acetate. After predetermined time, the total RNA were isolated from HL-60 cells and mRNA expression were quantitated by RT-PCR method. As a result, in the presence of serum and LPS stimulation, the expression of IL-1β mRNA increased with increasing the contact angle of films and reached to maximum at 70–80°. In the case of without LPS stimulation, IL-1β mRNA expression was decreased compared to the condition of presence of serum and LPS stimulation. From other results, IL-1β mRNA expression was influenced by the kind of substrate, LPS stimulation, serum presence and their combination. We assumed that RT-PCR method is one of the powerful methods to study the biocompatibility of materials.
10 schema:editor N59d5a47bcb1e46ed98b0f775d9a4bd9b
11 schema:genre chapter
12 schema:inLanguage en
13 schema:isAccessibleForFree false
14 schema:isPartOf N45ba46d4e6524414992865ab4f247953
15 schema:name Estimation of Biocompatibility of Polymeric Materials Using RT-PCR Method
16 schema:pagination 301-302
17 schema:productId N1465437dbc0d41739f99b752460f0373
18 N9b515aba953745268b56bd1386577ca6
19 Ncb5d8e172efb4cb08713fa23b7c96005
20 schema:publisher N7960f27e3bc4473ea699796b4b1cb7d8
21 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019786174
22 https://doi.org/10.1007/978-4-431-65883-2_87
23 schema:sdDatePublished 2019-04-15T18:10
24 schema:sdLicense https://scigraph.springernature.com/explorer/license/
25 schema:sdPublisher N76fa4534773148eeae84143141ac7d17
26 schema:url http://link.springer.com/10.1007/978-4-431-65883-2_87
27 sgo:license sg:explorer/license/
28 sgo:sdDataset chapters
29 rdf:type schema:Chapter
30 N1465437dbc0d41739f99b752460f0373 schema:name readcube_id
31 schema:value 5e45ab61a567798ecdcfac3c5b5e6a07c6be6a1328e33f02739da75b7a789e07
32 rdf:type schema:PropertyValue
33 N1d606f4d449440faac4d62a1d9444d4f rdf:first sg:person.013674666001.05
34 rdf:rest rdf:nil
35 N31f57482ed6d4139afc86528233aeb33 rdf:first sg:person.010753160655.67
36 rdf:rest N1d606f4d449440faac4d62a1d9444d4f
37 N45ba46d4e6524414992865ab4f247953 schema:isbn 978-4-431-65883-2
38 978-4-431-65885-6
39 schema:name Advanced Biomaterials in Biomedical Engineering and Drug Delivery Systems
40 rdf:type schema:Book
41 N45e09a8a8d1b4d708a37420a5b1abb9b rdf:first sg:person.012224463163.27
42 rdf:rest Nf278f1c15af44359b4551cd92afb610c
43 N59d5a47bcb1e46ed98b0f775d9a4bd9b rdf:first Nc7cb92ac5b2f4fc38333ab64cb7192f8
44 rdf:rest N5dc7c48bfe0947bb9c2786556925b166
45 N5dc7c48bfe0947bb9c2786556925b166 rdf:first Nbb3d1372b3a34522b8eaa1494dd49cc0
46 rdf:rest Nfeef9b3edaca45c5aa22b5b9bb022a42
47 N76fa4534773148eeae84143141ac7d17 schema:name Springer Nature - SN SciGraph project
48 rdf:type schema:Organization
49 N7960f27e3bc4473ea699796b4b1cb7d8 schema:location Tokyo
50 schema:name Springer Japan
51 rdf:type schema:Organisation
52 N7fb126835fbf4b1e841775708131c6c4 rdf:first sg:person.01050701273.52
53 rdf:rest N45e09a8a8d1b4d708a37420a5b1abb9b
54 N9b515aba953745268b56bd1386577ca6 schema:name doi
55 schema:value 10.1007/978-4-431-65883-2_87
56 rdf:type schema:PropertyValue
57 Nbb3d1372b3a34522b8eaa1494dd49cc0 schema:familyName Kim
58 schema:givenName Sung Wan
59 rdf:type schema:Person
60 Nc717c037c08d48808824c0c5ef800329 rdf:first Nec769fcff8c64490a7d36909c676a700
61 rdf:rest rdf:nil
62 Nc7cb92ac5b2f4fc38333ab64cb7192f8 schema:familyName Ogata
63 schema:givenName Naoya
64 rdf:type schema:Person
65 Ncb5d8e172efb4cb08713fa23b7c96005 schema:name dimensions_id
66 schema:value pub.1019786174
67 rdf:type schema:PropertyValue
68 Ncef33b14da164f4a8b66754e1a330951 schema:familyName Feijen
69 schema:givenName Jan
70 rdf:type schema:Person
71 Nec769fcff8c64490a7d36909c676a700 schema:familyName Okano
72 schema:givenName Teruo
73 rdf:type schema:Person
74 Nf278f1c15af44359b4551cd92afb610c rdf:first sg:person.0714140254.17
75 rdf:rest N31f57482ed6d4139afc86528233aeb33
76 Nfeef9b3edaca45c5aa22b5b9bb022a42 rdf:first Ncef33b14da164f4a8b66754e1a330951
77 rdf:rest Nc717c037c08d48808824c0c5ef800329
78 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
79 schema:name Chemical Sciences
80 rdf:type schema:DefinedTerm
81 anzsrc-for:0303 schema:inDefinedTermSet anzsrc-for:
82 schema:name Macromolecular and Materials Chemistry
83 rdf:type schema:DefinedTerm
84 sg:person.01050701273.52 schema:affiliation https://www.grid.ac/institutes/grid.258333.c
85 schema:familyName Kato
86 schema:givenName Shinya
87 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050701273.52
88 rdf:type schema:Person
89 sg:person.010753160655.67 schema:affiliation https://www.grid.ac/institutes/grid.258333.c
90 schema:familyName Sugimura
91 schema:givenName Kazuhisa
92 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010753160655.67
93 rdf:type schema:Person
94 sg:person.012224463163.27 schema:affiliation https://www.grid.ac/institutes/grid.258333.c
95 schema:familyName Ohmura
96 schema:givenName Kaori
97 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012224463163.27
98 rdf:type schema:Person
99 sg:person.013674666001.05 schema:affiliation https://www.grid.ac/institutes/grid.258333.c
100 schema:familyName Akashi
101 schema:givenName Mitsuru
102 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013674666001.05
103 rdf:type schema:Person
104 sg:person.0714140254.17 schema:affiliation https://www.grid.ac/institutes/grid.258333.c
105 schema:familyName Kishida
106 schema:givenName Akio
107 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0714140254.17
108 rdf:type schema:Person
109 https://doi.org/10.1002/jbm.820260702 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027630933
110 rdf:type schema:CreativeWork
111 https://doi.org/10.1016/0003-2697(87)90021-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013321498
112 rdf:type schema:CreativeWork
113 https://doi.org/10.1172/jci116548 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011103415
114 rdf:type schema:CreativeWork
115 https://www.grid.ac/institutes/grid.258333.c schema:alternateName Kagoshima University
116 schema:name Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, 1-21-40, Korimoto, Kagoshima 890, Japan
117 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...