Structure-Functional Analysis of Channelrhodopsins View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2015

AUTHORS

Hideaki E. Kato , Ryuichiro Ishitani , Osamu Nureki

ABSTRACT

Channelrhodopsin (ChR) was the first light-gated cation channel to be discovered from green algae. Since the inward flow of cations triggers neuron firing, neurons expressing ChRs can be optically controlled, even within freely moving mammals. Although ChR has been broadly applied to neuroscience research, little is known about its molecular mechanisms. In this chapter, we first describe the simple background of rhodopsin family proteins including ChR, and how optogenetics technology has been established since the discovery of ChR in 2002. We later introduce recent findings about the structure-functional relationship of ChR by especially focusing on a paper about the crystal structure of chimeric ChR (C1C2). After we explain the molecular architecture, the initial photoreactions, the ion-conducting pathway, and the putative channel gates of C1C2, we use three recent studies as examples to further explore the possibility of the structure-based engineering of ChR variants with properties that are more ideal for use as optogenetics tools. More... »

PAGES

31-45

References to SciGraph publications

  • 2011-01. Optogenetics in NATURE METHODS
  • 2014-04. X-ray crystal structure of voltage-gated proton channel in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2009-02. Bi-stable neural state switches in NATURE NEUROSCIENCE
  • 2012-12-12. Rapid regulation of depression-related behaviours by control of midbrain dopamine neurons in NATURE
  • 2010-03. Ultrafast optogenetic control in NATURE NEUROSCIENCE
  • 2011-09. Neocortical excitation/inhibition balance in information processing and social dysfunction in NATURE
  • 2002-10. Molecular basis of transmembrane signalling by sensory rhodopsin II–transducer complex in NATURE
  • 2002-01. X-ray structure of a ClC chloride channel at 3.0 Å reveals the molecular basis of anion selectivity in NATURE
  • 2012-02. Crystal structure of the channelrhodopsin light-gated cation channel in NATURE
  • 2011-07. The crystal structure of a voltage-gated sodium channel in NATURE
  • 1986-05. Cloning of the gene and cDNA for mammalian β-adrenergic receptor and homology with rhodopsin in NATURE
  • 2007-11. Crystal structure of the human β2 adrenergic G-protein-coupled receptor in NATURE
  • 2014-03. Independent optical excitation of distinct neural populations in NATURE METHODS
  • 2010-07. Regulation of parkinsonian motor behaviours by optogenetic control of basal ganglia circuitry in NATURE
  • 2007-11. Neural substrates of awakening probed with optogenetic control of hypocretin neurons in NATURE
  • 2011-04. Ultra light-sensitive and fast neuronal activation with the Ca2+-permeable channelrhodopsin CatCh in NATURE NEUROSCIENCE
  • 2010-11-11. Encoding of conditioned fear in central amygdala inhibitory circuits in NATURE
  • 2005-09. Millisecond-timescale, genetically targeted optical control of neural activity in NATURE NEUROSCIENCE
  • 1971-09. Rhodopsin-like Protein from the Purple Membrane of <i>Halobacterium halobium</i> in NATURE
  • Book

    TITLE

    Optogenetics

    ISBN

    978-4-431-55515-5
    978-4-431-55516-2

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-4-431-55516-2_3

    DOI

    http://dx.doi.org/10.1007/978-4-431-55516-2_3

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1042313755


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