Structural Analysis of the Sea Urchin Egg Cortex Isolated on a Substratum View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1988

AUTHORS

S. Yonemura , S. Tsukita , I. Mabuchi

ABSTRACT

A method for the isolation of the sea urchin egg cortex appropriate for preservation of the cortical structure was sought. In the cortex of an egg at the streak stage isolated by homogenization, the cortical vacuoles tended to be situated apart from the plasma membrane to some extent, making a room which well-developed actin filament meshworks occupied. However, in the intact egg at the streak stage, which was rapid-frozen and freeze-substituted, the vacuoles were observed close to the plasma membrane. Homogenization may have induced the formation of the meshwork. On the other hand, the isolation on a protamine-coated substratum was found to preserve the cortical structure in situ and used for the electron microscopic analysis of the structural changes of the cortical cytoskeleton. Short actin filaments in microvilli of the unfertilized egg cortex were demonstrated clearly for the first time. They were attached to the plasma membrane at their barbed ends and cross-linked with each other by thin strands. A dramatic actin polymerization which occurs soon after fertilization was observed as a formation of a filamentous layer underneath the plasma membrane. Subsequently, this layer reduced in thickness and microvillar actin filament bundles become prominent structures of the cortical cytoskeleton. These results supported previous studies using fluorescence microscopy. More... »

PAGES

104-115

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-7091-9011-1_12

DOI

http://dx.doi.org/10.1007/978-3-7091-9011-1_12

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006024877


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo, 153, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo, 153, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yonemura", 
        "givenName": "S.", 
        "id": "sg:person.012347332265.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012347332265.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Ultrastructural Research, The Tokyo Metropolitan Institute of Medical Sciences, Honkomagome, Bunkyo-ku, Tokyo, Japan", 
          "id": "http://www.grid.ac/institutes/grid.272456.0", 
          "name": [
            "Department of Ultrastructural Research, The Tokyo Metropolitan Institute of Medical Sciences, Honkomagome, Bunkyo-ku, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tsukita", 
        "givenName": "S.", 
        "id": "sg:person.015335234265.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015335234265.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo, 153, Japan", 
          "id": "http://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo, 153, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mabuchi", 
        "givenName": "I.", 
        "id": "sg:person.010064373571.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010064373571.04"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1988", 
    "datePublishedReg": "1988-01-01", 
    "description": "A method for the isolation of the sea urchin egg cortex appropriate for preservation of the cortical structure was sought. In the cortex of an egg at the streak stage isolated by homogenization, the cortical vacuoles tended to be situated apart from the plasma membrane to some extent, making a room which well-developed actin filament meshworks occupied. However, in the intact egg at the streak stage, which was rapid-frozen and freeze-substituted, the vacuoles were observed close to the plasma membrane. Homogenization may have induced the formation of the meshwork. On the other hand, the isolation on a protamine-coated substratum was found to preserve the cortical structure in situ and used for the electron microscopic analysis of the structural changes of the cortical cytoskeleton. Short actin filaments in microvilli of the unfertilized egg cortex were demonstrated clearly for the first time. They were attached to the plasma membrane at their barbed ends and cross-linked with each other by thin strands. A dramatic actin polymerization which occurs soon after fertilization was observed as a formation of a filamentous layer underneath the plasma membrane. Subsequently, this layer reduced in thickness and microvillar actin filament bundles become prominent structures of the cortical cytoskeleton. These results supported previous studies using fluorescence microscopy.", 
    "editor": [
      {
        "familyName": "Tazawa", 
        "givenName": "Masashi", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-3-7091-9011-1_12", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-3-7091-9013-5", 
        "978-3-7091-9011-1"
      ], 
      "name": "Cell Dynamics", 
      "type": "Book"
    }, 
    "keywords": [
      "plasma membrane", 
      "cortical cytoskeleton", 
      "streak stage", 
      "egg cortex", 
      "unfertilized egg cortex", 
      "sea urchin egg cortex", 
      "short actin filaments", 
      "actin filament bundles", 
      "actin polymerization", 
      "filament meshwork", 
      "actin filaments", 
      "actin filament meshwork", 
      "barbed ends", 
      "fluorescence microscopy", 
      "cytoskeleton", 
      "filament bundles", 
      "electron microscopic analysis", 
      "membrane", 
      "filamentous layer", 
      "vacuoles", 
      "substratum", 
      "eggs", 
      "microscopic analysis", 
      "intact eggs", 
      "cortical vacuoles", 
      "isolation", 
      "structural analysis", 
      "meshwork", 
      "thin strands", 
      "first time", 
      "fertilization", 
      "strands", 
      "previous studies", 
      "filaments", 
      "microvilli", 
      "prominent structure", 
      "formation", 
      "structural changes", 
      "stage", 
      "structure", 
      "homogenization", 
      "analysis", 
      "cortical structures", 
      "microscopy", 
      "bundles", 
      "changes", 
      "extent", 
      "situ", 
      "preservation", 
      "study", 
      "end", 
      "cortex", 
      "results", 
      "hand", 
      "polymerization", 
      "time", 
      "layer", 
      "method", 
      "thickness", 
      "room", 
      "urchin egg cortex", 
      "protamine-coated substratum", 
      "dramatic actin polymerization", 
      "microvillar actin filament bundles"
    ], 
    "name": "Structural Analysis of the Sea Urchin Egg Cortex Isolated on a Substratum", 
    "pagination": "104-115", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1006024877"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-3-7091-9011-1_12"
        ]
      }
    ], 
    "publisher": {
      "name": "Springer Nature", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-3-7091-9011-1_12", 
      "https://app.dimensions.ai/details/publication/pub.1006024877"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2021-12-01T20:13", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/chapter/chapter_94.jsonl", 
    "type": "Chapter", 
    "url": "https://doi.org/10.1007/978-3-7091-9011-1_12"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-3-7091-9011-1_12'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-3-7091-9011-1_12'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-3-7091-9011-1_12'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-3-7091-9011-1_12'


 

This table displays all metadata directly associated to this object as RDF triples.

141 TRIPLES      23 PREDICATES      90 URIs      83 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-3-7091-9011-1_12 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N6341c03a905d40a2b6ea457654e5a672
4 schema:datePublished 1988
5 schema:datePublishedReg 1988-01-01
6 schema:description A method for the isolation of the sea urchin egg cortex appropriate for preservation of the cortical structure was sought. In the cortex of an egg at the streak stage isolated by homogenization, the cortical vacuoles tended to be situated apart from the plasma membrane to some extent, making a room which well-developed actin filament meshworks occupied. However, in the intact egg at the streak stage, which was rapid-frozen and freeze-substituted, the vacuoles were observed close to the plasma membrane. Homogenization may have induced the formation of the meshwork. On the other hand, the isolation on a protamine-coated substratum was found to preserve the cortical structure in situ and used for the electron microscopic analysis of the structural changes of the cortical cytoskeleton. Short actin filaments in microvilli of the unfertilized egg cortex were demonstrated clearly for the first time. They were attached to the plasma membrane at their barbed ends and cross-linked with each other by thin strands. A dramatic actin polymerization which occurs soon after fertilization was observed as a formation of a filamentous layer underneath the plasma membrane. Subsequently, this layer reduced in thickness and microvillar actin filament bundles become prominent structures of the cortical cytoskeleton. These results supported previous studies using fluorescence microscopy.
7 schema:editor N7a5089cf842e4dcd905d345e3aa923d9
8 schema:genre chapter
9 schema:inLanguage en
10 schema:isAccessibleForFree false
11 schema:isPartOf Ne87d992de440410aa67f4bcefdda5839
12 schema:keywords actin filament bundles
13 actin filament meshwork
14 actin filaments
15 actin polymerization
16 analysis
17 barbed ends
18 bundles
19 changes
20 cortex
21 cortical cytoskeleton
22 cortical structures
23 cortical vacuoles
24 cytoskeleton
25 dramatic actin polymerization
26 egg cortex
27 eggs
28 electron microscopic analysis
29 end
30 extent
31 fertilization
32 filament bundles
33 filament meshwork
34 filamentous layer
35 filaments
36 first time
37 fluorescence microscopy
38 formation
39 hand
40 homogenization
41 intact eggs
42 isolation
43 layer
44 membrane
45 meshwork
46 method
47 microscopic analysis
48 microscopy
49 microvillar actin filament bundles
50 microvilli
51 plasma membrane
52 polymerization
53 preservation
54 previous studies
55 prominent structure
56 protamine-coated substratum
57 results
58 room
59 sea urchin egg cortex
60 short actin filaments
61 situ
62 stage
63 strands
64 streak stage
65 structural analysis
66 structural changes
67 structure
68 study
69 substratum
70 thickness
71 thin strands
72 time
73 unfertilized egg cortex
74 urchin egg cortex
75 vacuoles
76 schema:name Structural Analysis of the Sea Urchin Egg Cortex Isolated on a Substratum
77 schema:pagination 104-115
78 schema:productId N0a82630d01174a4cba9f34eff213d67d
79 N17a9db5c1d064ac3ab1e47bc2d312507
80 schema:publisher Nd8cf098c64bd4db6a671e4271271714d
81 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006024877
82 https://doi.org/10.1007/978-3-7091-9011-1_12
83 schema:sdDatePublished 2021-12-01T20:13
84 schema:sdLicense https://scigraph.springernature.com/explorer/license/
85 schema:sdPublisher Nbf28e3204f8b45e9b90200aab62c1c00
86 schema:url https://doi.org/10.1007/978-3-7091-9011-1_12
87 sgo:license sg:explorer/license/
88 sgo:sdDataset chapters
89 rdf:type schema:Chapter
90 N0a82630d01174a4cba9f34eff213d67d schema:name dimensions_id
91 schema:value pub.1006024877
92 rdf:type schema:PropertyValue
93 N17a9db5c1d064ac3ab1e47bc2d312507 schema:name doi
94 schema:value 10.1007/978-3-7091-9011-1_12
95 rdf:type schema:PropertyValue
96 N2390514cf1e04ee5aae1ab6e664ae9be rdf:first sg:person.010064373571.04
97 rdf:rest rdf:nil
98 N6341c03a905d40a2b6ea457654e5a672 rdf:first sg:person.012347332265.40
99 rdf:rest N7dab1f939be240fe9531ac3c6951430d
100 N7a5089cf842e4dcd905d345e3aa923d9 rdf:first Nfa5a0f5ccaa546d9a27d71dd9fe06702
101 rdf:rest rdf:nil
102 N7dab1f939be240fe9531ac3c6951430d rdf:first sg:person.015335234265.05
103 rdf:rest N2390514cf1e04ee5aae1ab6e664ae9be
104 Nbf28e3204f8b45e9b90200aab62c1c00 schema:name Springer Nature - SN SciGraph project
105 rdf:type schema:Organization
106 Nd8cf098c64bd4db6a671e4271271714d schema:name Springer Nature
107 rdf:type schema:Organisation
108 Ne87d992de440410aa67f4bcefdda5839 schema:isbn 978-3-7091-9011-1
109 978-3-7091-9013-5
110 schema:name Cell Dynamics
111 rdf:type schema:Book
112 Nfa5a0f5ccaa546d9a27d71dd9fe06702 schema:familyName Tazawa
113 schema:givenName Masashi
114 rdf:type schema:Person
115 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
116 schema:name Biological Sciences
117 rdf:type schema:DefinedTerm
118 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
119 schema:name Biochemistry and Cell Biology
120 rdf:type schema:DefinedTerm
121 sg:person.010064373571.04 schema:affiliation grid-institutes:grid.26999.3d
122 schema:familyName Mabuchi
123 schema:givenName I.
124 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010064373571.04
125 rdf:type schema:Person
126 sg:person.012347332265.40 schema:affiliation grid-institutes:grid.26999.3d
127 schema:familyName Yonemura
128 schema:givenName S.
129 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012347332265.40
130 rdf:type schema:Person
131 sg:person.015335234265.05 schema:affiliation grid-institutes:grid.272456.0
132 schema:familyName Tsukita
133 schema:givenName S.
134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015335234265.05
135 rdf:type schema:Person
136 grid-institutes:grid.26999.3d schema:alternateName Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo, 153, Japan
137 schema:name Department of Biology, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo, 153, Japan
138 rdf:type schema:Organization
139 grid-institutes:grid.272456.0 schema:alternateName Department of Ultrastructural Research, The Tokyo Metropolitan Institute of Medical Sciences, Honkomagome, Bunkyo-ku, Tokyo, Japan
140 schema:name Department of Ultrastructural Research, The Tokyo Metropolitan Institute of Medical Sciences, Honkomagome, Bunkyo-ku, Tokyo, Japan
141 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...