Cytokine Marker Measurement in Human Neuroblastoma Cells with Supersensitive and Multiplex Assay: MUSTag Technology View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2013

AUTHORS

Ryoichi Miyashita , Li Chen , Yoshihito Morizane , Yuji Takeshita , Hidekimi Fukui , Taku Ishizaki , Kiyoshi Hatakeyama , Kiyoshige Ohseto , Futoshi Shibasaki , Hiroyuki Uchino

ABSTRACT

BACKGROUND: Recently, various sets of protein -biomarkers have been discovered in important diseases such as cancers, brain stroke and heart attack. However, clinical validation is difficult and time-consuming by individual assays or because of very low concentrations at early stages of the diseases. We have developed assay technology through an innovative modification of the immuno-PCR method for the super-sensitive and multiplex detection of target biomarkers. METHODS: In the assay technology, each different oligo-tag simultaneously detects multiplex protein targets with extremely high-level sensitivity in a dose-dependent manner by qRT-PCR (maximum: three plexes). In this study, we measured specific secreted protein concentrations in the culture supernatant of a 24-h culture of transfected SH-SY5Y cells with MUSTag. RESULTS: There was a significant increase in the protein level of tumor necrosis factor (TNF)-α measured with extremely high-level sensitivity (≥10 pg/mL). Compared with negative controls, the levels of TNF-α increased from 16.9 to 28.1 pg/mL (p = 0.011). CONCLUSION: We suggest that our assay technology might be of clinical value in treating patients with cancer, cerebral ischemia, or patients who need a prompt and predictive diagnosis for adequate treatment. More... »

PAGES

317-20

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-7091-1434-6_62

DOI

http://dx.doi.org/10.1007/978-3-7091-1434-6_62

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1025748817

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23564157


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