The Functional Interaction Trap: A Novel Strategy to Study Specific Protein-Protein Interactions View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2004

AUTHORS

Alok Sharma , Susumu Antoku , Bruce J. Mayer

ABSTRACT

Protein-protein interactions play a central role in almost all aspects of the living cell, and understanding these interactions holds the key to understanding a host of cellular processes. Whether it is an enzyme modifying its substrate, the assembly of subunits of a multiprotein complex, the recognition and binding of a specific ligand, or the polymerization of monomeric subunits such as those of actin, protein-protein interactions are essential for regulating and organizing virtually all physiological responses. Much recent research has focused on understanding these interactions in detail, and while progress has been rapid in many areas, it is clear that new tools to study specific protein-protein interactions are sorely needed. For example, our ability to identify actual or potential protein-protein interactions has outpaced our ability to validate the functional significance of those interactions, or of post-translational modifications that might result from those interactions. In this article we will discuss the Functional Interaction Trap (FIT) approach [13, 44], a novel proteomic tool designed to elucidate the physiological outputs that are mediated by specific protein-protein interactions or post-translational modifications in cellular signaling. We will also discuss in detail a specific application of the FIT approach, where it is used to dissect the functional consequences of tyrosine phosphorylation of specific substrates in the cell. More... »

PAGES

165-182

Book

TITLE

Methods in Proteome and Protein Analysis

ISBN

978-3-642-05779-3
978-3-662-08722-0

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-662-08722-0_11

DOI

http://dx.doi.org/10.1007/978-3-662-08722-0_11

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034987680


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