Construction of scFv from Hybridoma by Two-Step Cloning View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2001

AUTHORS

Frank Breitling , Dieter Moosmayer , Bodo Brocks , Stefan Dübel

ABSTRACT

Despite the fact that a growing number of recombinant antibodies has been isolated from phage display libraries, still many antibody specificities are available from hybridoma cell lines. Here, a method is presented to obtain the genetic information for the antigen binding part of the antibody from hybridoma cells, and to assemble it into a functional bacterially expressed fusion protein (scFv fragment). To achieve this, vectors have been constructed which combine the two variable regions (Vh and Vl) with a peptide linker to yield an scFv fragment. The genetic information for Vh and Vl is amplified from hybridoma cells using the polymerase chain reaction (PCR) with antibody specific primers. More... »

PAGES

41-55

References to SciGraph publications

Book

TITLE

Antibody Engineering

ISBN

978-3-540-41354-7
978-3-662-04605-0

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-662-04605-0_3

DOI

http://dx.doi.org/10.1007/978-3-662-04605-0_3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014007988


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