Immunomagnetic Particles for Cell Isolation View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1993

AUTHORS

C. Andreoni

ABSTRACT

In vitro studies of cell-cell interactions and regulation of cellular functions require cell subsets of high purity. With conventional techniques for immunological cell separation, such as resetting (Gold, 67), complement lysis (Loski, 83) or panning (Maage, 77), the amount of reagent and the time required for the separation is considerable and out of proportion to the quality of the separation. Fluorescence-activated cell sorting (FACS) (Parks, 86) is a powerful method for the isolation of subpopulations, but application is often limited by the small separation capacity of approximately 107 cells per hour. A recent, alternative cell sorting method involves immunomagnetic particles (Rembaum, 88). Why immunomagnetic particles ? Because the coated surface conserves the advantage of monoclonal selectivity. Because the mobile particle allows rapid reaction with its antigen. For example, the time needed for an antibody against placental alkaline phosphatase to bind the enzyme was only 5min, when the antibody was bound to particles, but it took 24hr to bind the same amount of enzyme when the same amount of antibody was bound to the wall of a microtiter well (Millan, 85). Because magnetic particles can be concentrated easily by a simple external permanent magnet. This property allows easy handling of large number of cells and introduces no problems regarding sterility. Since the first immunomagnetic cell separation (Molday, 77) different immunomagnetic devices have been developed (Lievestad, 86; Hebell, 89; Abts, 89; Autran, 91; Liberti, 91) and allows the optimization of the cell sorting efficiency. Besides giving details of our own experience in immunomagnetic sorting, essential properties of particles and caracteristics of particle-cell interactions are presented. More... »

PAGES

433-450

Book

TITLE

Flow Cytometry

ISBN

978-3-642-84618-2
978-3-642-84616-8

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-84616-8_29

DOI

http://dx.doi.org/10.1007/978-3-642-84616-8_29

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016030239


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