Ribosomal RNA Sequencing View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1991

AUTHORS

Michel Solignac

ABSTRACT

Ribosomal genes can be cloned or PCR amplified and then sequenced as other DNA sequences. However, the products of the genes, ribosomal RNAs, can be used as template for direct sequencing with the reverse transcriptase as polymerase following a protocol derived from the Sanger’s technics for DNA (dideoxynucleotide chain termination). Universal primers can be used to sequence the chosen region of the rRNA. More... »

PAGES

369-377

Book

TITLE

Molecular Techniques in Taxonomy

ISBN

978-3-642-83964-1
978-3-642-83962-7

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-83962-7_24

DOI

http://dx.doi.org/10.1007/978-3-642-83962-7_24

DIMENSIONS

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