Preparation and Visualization of Mitochondrial DNA for RFLP Analysis View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1991

AUTHORS

Michel Solignac

ABSTRACT

The principal problem in preparation of mitochondrial DNA is to avoid contamination by nuclear DNA. One strategy is to prepare a highly purified fraction of mitochondria, using cellular fractionation (differential centrifugation, sucrose gradients). Another is to prepare a total extract of DNA (or an extract solely enriched in mtDNA) and to separate nuclear and mitochondrial DNA, taking advantage of their differential physical properties (physical conformation, density). The last possibility is to work with the mixture of DNA.s from the total extract and to use a probe, complementary of the mtDNA. More... »

PAGES

295-319

Book

TITLE

Molecular Techniques in Taxonomy

ISBN

978-3-642-83964-1
978-3-642-83962-7

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-83962-7_19

DOI

http://dx.doi.org/10.1007/978-3-642-83962-7_19

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1025890834


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