A Multiplex Amplification Approach for Simultaneous Typing of Five Loci in DNA of Ancient Basque Populations View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1996

AUTHORS

Prieto , E. Arroyo , A. Pérez-Pérez , C. Asperilla , I. Arenal , J. M. Ruiz de la Cuesta , D. Turbón

ABSTRACT

We tried to test the commercial forensic kit worldwide known as “Polymarker” in cases of samples of very old DNA. We will try to type ancient DNA samples for five genetic systems (LDLR, GYPA, HBGG, D7S8 and GC) all of them comprised in this multiplex PCR commercial kit. Our hypothesis is that given that the amount of target ancient DNA is very small, only standardized systems already tested for succesful amplification of a few nanograms can be suitable for very scarce and damaged DNA. This kit, in the routine forensic casework, produces good results with a template DNA amount of 2–40 ngr in a final reaction volume of 100 μl. More... »

PAGES

204-206

References to SciGraph publications

Book

TITLE

16th Congress of the International Society for Forensic Haemogenetics (Internationale Gesellschaft für forensische Hämogenetik e.V.), Santiago de Compostela, 12–16 September 1995

ISBN

978-3-540-60492-1
978-3-642-80029-0

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-80029-0_56

DOI

http://dx.doi.org/10.1007/978-3-642-80029-0_56

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024248467


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