Light and electron microscopy in microbial mat research: An overview View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1994

AUTHORS

John F. Stolz

ABSTRACT

A variety of traditional techniques for light microscopy (e.g., differential interference contrast, phase contrast, fluorescence) and electron microscopy (transmission and scanning) have been used to obtain valuable information on the structure and species composition of microbial mat communities. Recent advances in techniques and instrumentation for microscopy are providing new tools for studying these communities in situ. Innovations in immunohistochemistry and molecular biology allow in situ labeling of specific microorganisms with fluorophores or gold particles which have been conjugated to antibodies or oligonucleotides. Digital image analysis software and confocal laser microscopy have made serial sections and 3-D reconstruction possible, providing structural data on biofilms, microbial mats, and microfossils in petrographic sections. This review will highlight a few examples of these new technologies with emphasis on those which have potential use in microbial mat studies. More... »

PAGES

173-182

References to SciGraph publications

Book

TITLE

Microbial Mats

ISBN

978-3-642-78993-9
978-3-642-78991-5

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-78991-5_18

DOI

http://dx.doi.org/10.1007/978-3-642-78991-5_18

DIMENSIONS

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