1994
AUTHORS ABSTRACTUltraviolet (UV) light causes photolesions in DNA that induce genetic mutations (Friedberg 1985; Hutchinson 1987; Sage 1993). One of the well-characterized photolesions is cyclobutane pyrimidine dimers, which are produced by irradiation at wavelengths close to the absorption maximum of DNA (about 260 nm). This photoproduct has a cyclobutane ring formed by covalent bonds at the C5 and C6 positions between two adjacent pyrimidine bases, and the major isomer in DNA duplexes in cis-syn (Scheme 1). This type of lesion has been reported to induce mutations in vivo (Jiang and Taylor 1993; Kamiya et al. 1993). UV irradiation predominantly induces transitions from cytosine to thymine at sequences of adjacent pyrimidines (Miller 1985; Schaaper et al. 1987; Jiang and Taylor 1993). In our study, however, transversions from thymine to adenine occurred most frequently on the 3′-side of the cis-syn thymine dimer or on the 5′-side of the trans-syn thymine dimer, and these mutations in the ras oncogene induced focus-formation of NIH3T3 cells (Kamiya et al. 1993). More... »
PAGES217-226
Nucleic Acids and Molecular Biology
ISBN
978-3-642-78668-6
978-3-642-78666-2
http://scigraph.springernature.com/pub.10.1007/978-3-642-78666-2_12
DOIhttp://dx.doi.org/10.1007/978-3-642-78666-2_12
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