Helicobacter pylori Detection by Polymerase Chain Reaction of the Gene Encoding 16S Ribosomal RNA in Fresh and Paraffin-Embedded Material View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1993

AUTHORS

S. A. Ho , F. A. Lewis , D. A. Secker , D. S. Tompkins , G. R. Taylor , J. I. Wyatt , M. F. Dixon , P. Quirke

ABSTRACT

The emergence of Helicobacter pylori as an important aetiological agent in gastroduodenal disease has led to the introduction of many methods for the detection of this organism. Current methods for H. pylori detection can be divided into two main categories, endoscopic and non-endoscopic. Endoscopy provides biopsy specimens for subsequent culture [3], histology [5], in situ hybridisation [20] or rapid urease testing [10]. The non-endoscopic techniques include serological tests [12] and the 14C or 13C breath tests [4, 23]. All of these techniques are unsatisfactory because they are insensitive to low bacterial densities and cannot identify “non-culturable” coccoid forms of H. pylori. Research has been hampered through the lack of a sensitive assay and the invasive nature of the best methods, and thus many key questions remain unresolved. The development of an extremely sensitive technique which can be applied to a wide variety of materials (including saliva or faeces), may allow the sources and routes of transmission of H. pylori to be elucidated. More... »

PAGES

3-11

References to SciGraph publications

Book

TITLE

Helicobacter pylori and Gastroduodenal Pathology

ISBN

978-3-642-77488-1
978-3-642-77486-7

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-77486-7_1

DOI

http://dx.doi.org/10.1007/978-3-642-77486-7_1

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