Agarose Plating of Protoplasts and its Applications View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1989

AUTHORS

J. J. M. Dons , C. M. Colijn-Hooymans

ABSTRACT

Successful regeneration of plants from protoplasts involves a series of important events. Several steps in the process can be discriminated which all have their own, often empirically determined, requirements. These different steps and the parameters that have been shown to be important in the process are described in Table 1.

PAGES

50-62

References to SciGraph publications

  • 1971-03. Plating of isolated tobacco mesophyll protoplasts on agar medium in PLANTA
  • 1987-06. Regeneration of leaf mesophyll protoplasts of tomato cultivars (L. esculentum): factors important for efficient protoplast culture and plant regeneration in PLANT CELL REPORTS
  • 1987-01. The use of agarose bead culture for the regeneration of single cell-derived colonies from protoplasts isolated from suspension cultures of Humulus lupulus in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1983-08. A new procedure for increasing efficiency of protoplast plating and clone selection in PLANT CELL REPORTS
  • 1985-02. A simple culture method for Brassica hypototyl protoplasts in PLANT CELL REPORTS
  • 1983-09. Improved protoplast culture and agarose media in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1985-02. Immobilization of plant protoplasts: Viability studies in PLANT CELL REPORTS
  • 1985-10. In vitro culture of Cucumis sativus L. V. Stabilizing effect of glycine on leaf protoplasts in PLANT CELL REPORTS
  • 1985-05. Gene transfer to cereal cells mediated by protoplast transformation in MOLECULAR GENETICS AND GENOMICS
  • 1986-02. Integration of foreign DNA following microinjection of tobacco mesophyll protoplasts in MOLECULAR GENETICS AND GENOMICS
  • 1985-05. Uptake, integration, expression and genetic transmission of a selectable chimaeric gene by plant protoplasts in MOLECULAR GENETICS AND GENOMICS
  • 1986-08. Direct somatic embryogenesis from protoplasts of Foeniculum vulgare in PLANT CELL REPORTS
  • 1986-12. Efficient Plant Regeneration from Rice Protoplasts Through Somatic Embryogenesis in NATURE BIOTECHNOLOGY
  • 1987-04. Formation of callus and somatic embryos from protoplasts of a commercial hybrid of maize (Zea mays L.) in THEORETICAL AND APPLIED GENETICS
  • 1987-01. Somatic hybridization of amino acid analogue-resistant cell lines of potato (Solanum tuberosum L.) by electrofusion in THEORETICAL AND APPLIED GENETICS
  • 1983-06. Protoplast derived tobacco cells can survive capillary microinjection of the fluorescent dye Lucifer Yellow in PROTOPLASMA
  • 1986-08. Isolation, culture and callus regeneration of protoplasts of wild and cultivated Helianthus species in PLANT CELL REPORTS
  • 1986-09. Microcallus formation from maize protoplasts prepared from embryogenic callus in PLANTA
  • 1986-08. Plant regeneration from mesophyll protoplasts in commercial potato cultivars (Primura, Kennebec, Spunta, Desirée) in PLANT CELL REPORTS
  • 1985-02. A method for the microinjection and culture of protoplasts at very low densities in PLANT CELL REPORTS
  • 1983-10. Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species in PLANT CELL REPORTS
  • 1985-10. Induction of efficient cell division in alfalfa protoplasts in PLANT CELL REPORTS
  • 1986-04. Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.) in PLANT CELL REPORTS
  • 1987-06. Plant regeneration from protoplasts of a wild lettuce species (Lactuca saligna L.) in PLANT CELL REPORTS
  • 1983. Protoplast Culture and Plant Regeneration of Cereals and Other Recalcitrant Crops in PROTOPLASTS 1983
  • 1983. Recent Developments in the Culture and Regeneration of Plant Protoplasts in PROTOPLASTS 1983
  • 1985-06. Direct gene transferState of the Art and Future Potential in PLANT MOLECULAR BIOLOGY REPORTER
  • Book

    TITLE

    Plant Protoplasts and Genetic Engineering I

    ISBN

    978-3-642-73616-2
    978-3-642-73614-8

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-3-642-73614-8_4

    DOI

    http://dx.doi.org/10.1007/978-3-642-73614-8_4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1036304448


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