Fate of Bacterial DNA Injected into Embryos of Poeciliid Fish View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1973

AUTHORS

J. Vielkind , M. Schwab , F. Anders

ABSTRACT

Since Avery, MacLeod and McCarty (1944) and later on other investigators (for review, see Hotchkiss, 1952; Schaeffer, 1964) could successfully demonstrate that genetic transformation in bacteria is caused by DNA externally added to bacterial cultures, many experiments had been done to achieve genetic transformation in higher organisms also. Whereas the results of the first experiments carried out by Benoit et al. (1957) on ducklings were judged to be positive, the findings of further experiments on the same as well as on other subjects could not be interpreted as genetic transformation (for review, see Ledoux, 1965). In the following years, however, it became possible to do genetic transformation of eukaryotic cells at least in tissue culture (Szybalska and Szybalski, 1962) and, only recently, in some cases also in intact higher organisms (for review, see Hess, 1972; collected literature in Ledoux, 1971, and in Raspé, 1972). It had been possible, thereby, to show that the applied DNA was integrated into the host cell genome. But up to now, nobody had been able to show simultaneously that the integrated DNA was transcribed and translated, nor had it been proved that a new phenotype which occurred after DNA treatment was the result of integration, transcription, and translation, of the extraneous DNA. More... »

PAGES

123-137

References to SciGraph publications

Book

TITLE

Genetics and Mutagenesis of Fish

ISBN

978-3-642-65702-3
978-3-642-65700-9

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-65700-9_13

DOI

http://dx.doi.org/10.1007/978-3-642-65700-9_13

DIMENSIONS

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