1996
AUTHORS ABSTRACTHigh temperature tolerance is one of the main components of yield stability in crop plants. The genetic mechanisms controlling variability within and between species remain largely unknown, even though their genetical basis is expected to be complex. Therefore the genetic dissection of the character requires adequate methods of analysis. A two step strategy was followed: i) character dissection into physiological components, such as cellular membrane stability (CMS), heat shock protein (HSP) synthesis, pollen germination and tube growth, root elongation; ii) genetic dissection of these components by means of molecular markers, such as RFLPs, used to detect QTLs (Quantitative Trait Loci) controlling each trait. All analyses were performed on a population of 45 recombinant inbred lines (RI) derived by two contrasting inbreds. In each case, except for HSPs, thermotolerance was evaluated as degree of injury of stressed versus non stressed material. HSP expression in stressed seedling roots was measured quantitatively by reading each HSP band after ID electrophoresis with a densitometric scanner. CMS was evaluated as electrolyte leakage from leaf disks. For all traits a significant difference among the RI genotypes was found. By RFLP analysis from 3 to 7 QTLs were identified for the different components. A comparison between all the QTLs detected revealed that some of them appear to be in common between the physiological components of thermotolerance, thus opening the way to marker assisted selection procedures. More... »
PAGES31-38
Physical Stresses in Plants
ISBN
978-3-642-64732-1
978-3-642-61175-9
http://scigraph.springernature.com/pub.10.1007/978-3-642-61175-9_3
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