Interaction Between Osmotic and Hydrostatic Pressure in Yeast Inactivation View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1999

AUTHORS

J. M. Perrier-Cornet , M. Hayert , E. Saurat , C. Milesse , P. Gervais

ABSTRACT

The protective effect of an osmotic perturbation on high-pressure inactivation has been investigated using a yeast strain:Saccharomyces cerevisiae. Osmotic shocks have been carried out with binary medium (glycerol/sorbitol and water) with or without glucose. Variations of osmotic level, osmotic shift kinetics and time between osmotic and hydrostatic treatment (350 MPa/10 min) have allowed the characterization of cell baroprotection. Two different cumulative baroprotection effects have been distinguished: one conferred by the solute on biological structures and the other related to the osmotic stress, cell volume variations and stress metabolism response (HSP, trehalose). These experiments have also exhibited a repair phase, which occurred immediately after the high-pressure treatment and required the presence of glucose. More... »

PAGES

27-30

Book

TITLE

Advances in High Pressure Bioscience and Biotechnology

ISBN

978-3-642-64300-2
978-3-642-60196-5

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-60196-5_5

DOI

http://dx.doi.org/10.1007/978-3-642-60196-5_5

DIMENSIONS

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