Direct Analysis of Protein Complexes View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2000

AUTHORS

J. R. Yates , A. J. Link , D. Schieltz , J. K. Eng

ABSTRACT

Deciphering the functions of genes discovered by genome sequencing will be a major challenge of the post-genome era. Physiological processes are performed primarily by proteins and these functions are often accomplished with other proteins as components of multi-protein complexes, as part of signal transduction pathways or as ligands for receptors. Identifying the sets of proteins involved in a process will be key to understanding the individual roles of proteins. Approaches to accomplish this goal will encompass at least two types of measurements. First, by measuring expression levels of proteins under different cellular conditions and states, co-regulation of protein expression can be observed. Co-regulation will not necessarily indicate proteins are part of a complex or pathway, but that their functions are regulated as part of the process. A second measurement will encompass dissection of the components of protein complexes. Proteins perform many of their functions in concert with other proteins, by forming stable complexes or through more subtle interactions. A protein’s presence in a complex is more reflective of direct involvement in a process then association by co-regulation. Some proteins in complexes may be co-regulated and others may not be. By identifying the interacting proteins as a function of cellular state, insight into the networks of proteins involved in those processes will be obtained. More... »

PAGES

53-64

Book

TITLE

Proteome and Protein Analysis

ISBN

978-3-642-64060-5
978-3-642-59631-5

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-59631-5_4

DOI

http://dx.doi.org/10.1007/978-3-642-59631-5_4

DIMENSIONS

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