HER2/neu Gene Amplification Quantified by PCR and Melting Peak Analysis Using a Single Base Alteration Competitor as an Internal Standard View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2001

AUTHORS

Elaine Lyon , Alison Millson , Arminda Suli

ABSTRACT

The HER2/neu gene is amplified in 25–30% of primary breast cancers and correlates with relapse and shorter survival time (1,2,3,4,5). HER2/neu status may also impact therapeutic decisions since monoclonal antibodies against HER2/neu have shown inhibition of tumor growth when combined with traditional chemotherapy (6). Due to its prognostic significance and potential in therapeutic decisions, molecular methods for HER2/neu gene quantification are being developed. Recent advances in PCR technology using real-time fluorescent monitoring capabilities of the LightCycler allow mutation detection and quantification. Fluorescent hybridization probes have been designed to monitor product accumulation during PCR for real time detection (7). Hybridization probes can also distinguish a single base change by differences in melting temperature (Tm) as the probe melts from the wild-type or mutant allele (8,9,10,11). More... »

PAGES

207-217

Book

TITLE

Rapid Cycle Real-Time PCR

ISBN

978-3-540-66736-0
978-3-642-59524-0

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-59524-0_23

DOI

http://dx.doi.org/10.1007/978-3-642-59524-0_23

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029543447


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