Protein Identification by SEQUEST View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2001

AUTHORS

David L. Tabb , Jimmy K. Eng , John R. Yates

ABSTRACT

Sequencing peptides was an enormously cumbersome process until the development of tandem mass spectrometry (MS/MS). The main barrier to broad use of this technique has been the difficulty of spectrum interpretation. The SEQUEST software package attempts to ease this process by automatically matching tandem mass spectra to database sequences (Eng et al. 1994). Candidate sequences are culled from the database by several simple filters. Virtual spectra are constructed for these sequences, and these are compared to the observed spectrum. Sequences yielding spectra most similar to the observed one are reported to the user. More... »

PAGES

125-142

Book

TITLE

Proteome Research: Mass Spectrometry

ISBN

978-3-540-67256-2
978-3-642-56895-4

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-56895-4_7

DOI

http://dx.doi.org/10.1007/978-3-642-56895-4_7

DIMENSIONS

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