Development of One-Step RT-PCR for the Detection of an RNA Virus, Dengue, on the Capillary Real-Time Thermocycler View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2002

AUTHORS

Boon-Huan Tan , E’Ein See , Elizabeth Lim , Eric Peng-Huat Yap

ABSTRACT

Human infection with Dengue virus, an arthropod-borne virus, is a common and re-emerging disease in many tropical regions. In its most severe form, Dengue haemorrhagic fever, the resulting virus-induced coagulopathy is potentially lethal. However, classic clinical signs of Dengue infection appear only late, in the postfebrile phase of infection, and many subclinical episodes of infection remain asymptomatic and difficult to detect. There is a need to develop rapid diagnostic tests to detect early and subclinical Dengue infection for treatment on the individual level, and for preventive public health measures. Although the gold standard for Dengue detection depends on the serological detection of Dengue-specific antibodies and/or Dengue virus isolation by cell culture [1], molecular-based detection methods such as conventional polymerase chain reaction (PCR) assays have been published [2, 3, 4, 5, 6, 7]. While sensitive, these assays involve many hands-on steps and are hence costly, manual and prone to carryover contamination and errors. More... »

PAGES

241-248

Book

TITLE

Rapid Cycle Real-Time PCR — Methods and Applications

ISBN

978-3-642-48353-0
978-3-642-48351-6

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-48351-6_24

DOI

http://dx.doi.org/10.1007/978-3-642-48351-6_24

DIMENSIONS

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