Occurrence, Diversity of CRISPR-Cas Systems and Genotyping Implications View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012-12-13

AUTHORS

Christine Pourcel , Christine Drevet

ABSTRACT

This chapter describes the overall variability of CRISPR-Cas systems as observed in publicly available genomes and how this can be used to draw hypotheses on phylogenetic relationships between species and between strains of a given species. The fact that spacers are added sequentially at the leader end and that a given spacer is rarely acquired twice or duplicated are key elements for building hierarchical relationships between strains. Presence/absence of a given CRISPR locus and variability in the number of direct repeats and spacers in that locus between strains have been frequently reported, providing in some cases phylogenic information, but this polymorphism was extensively used for genotyping in only a few instances. The observation that not all strains possess a CRISPR locus in a given species precludes its use as a general typing tool. However, in some species, the degree of variability is a powerful marker of the species diversity and evolution. Through examples found among 1,434 published genomes of bacteria and archaea, different features of the CRISPR-Cas systems diversity will be highlighted. More... »

PAGES

33-59

Book

TITLE

CRISPR-Cas Systems

ISBN

978-3-642-34656-9
978-3-642-34657-6

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-34657-6_2

DOI

http://dx.doi.org/10.1007/978-3-642-34657-6_2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1008152367


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