Establishing an Animal Model of Dupuytren’s Contracture by Profiling Genes Associated with Fibrosis View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2012

AUTHORS

Latha Satish , Mark E. Baratz , Bradley Palmer , Sandra Johnson , J. Christopher Post , Garth D. Ehrlich , Sandeep Kathju

ABSTRACT

Dupuytren’s disease (DD) is characterized by the progressive development of a scar-like collagen-rich cord within the palmar fascia of the hand that results in permanent finger contracture. Currently, DD is most commonly treated by surgical resection of the diseased tissue. To date, no directly analogous animal model has been described in which to evaluate potential nonsurgical treatments for Dupuytren’s contracture. In the present study, we endeavor to establish an animal model that may ultimately serve as a platform in which to compare various treatments for DD. Hypothesizing that fibroblasts are the cellular effectors of the Dupuytren’s phenotype, we have transplanted fibroblasts derived from DD palmar fascia into the forepaw of nude rats. Our initial examinations showed that DD-derived fibroblasts persisted successfully in the forepaws of nude rats up to 56 days. Initial studies with quantitative real-time RT-PCR (qRT-PCR) using RNA derived from forepaw tissues harvested at 8 weeks showed that mRNA levels of alpha-smooth muscle actin (α-SMA) and type I collagen were significantly elevated in the forepaws of nude rats injected with DD-derived fibroblasts. The increase in α-SMA suggests an in vivo fibroblast to myofibroblast transformation that ultimately may result in scar formation and contracture. More... »

PAGES

101-108

Book

TITLE

Dupuytren’s Disease and Related Hyperproliferative Disorders

ISBN

978-3-642-22696-0
978-3-642-22697-7

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-22697-7_13

DOI

http://dx.doi.org/10.1007/978-3-642-22697-7_13

DIMENSIONS

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37 schema:description Dupuytren’s disease (DD) is characterized by the progressive development of a scar-like collagen-rich cord within the palmar fascia of the hand that results in permanent finger contracture. Currently, DD is most commonly treated by surgical resection of the diseased tissue. To date, no directly analogous animal model has been described in which to evaluate potential nonsurgical treatments for Dupuytren’s contracture. In the present study, we endeavor to establish an animal model that may ultimately serve as a platform in which to compare various treatments for DD. Hypothesizing that fibroblasts are the cellular effectors of the Dupuytren’s phenotype, we have transplanted fibroblasts derived from DD palmar fascia into the forepaw of nude rats. Our initial examinations showed that DD-derived fibroblasts persisted successfully in the forepaws of nude rats up to 56 days. Initial studies with quantitative real-time RT-PCR (qRT-PCR) using RNA derived from forepaw tissues harvested at 8 weeks showed that mRNA levels of alpha-smooth muscle actin (α-SMA) and type I collagen were significantly elevated in the forepaws of nude rats injected with DD-derived fibroblasts. The increase in α-SMA suggests an in vivo fibroblast to myofibroblast transformation that ultimately may result in scar formation and contracture.
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