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            "Chirurgische Klinik I, Universit\u00e4tsklinikum Benjamin Franklin, Deutschland"
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        "id": "https://doi.org/10.1136/gut.43.4.499", 
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    "description": "Apart of the immunological reaction pouchitis shows many similarities to ulcerative colitis. Concerning the cytokine expression it is well known that during pouchitis the proinflammatory interleukines IL 1-\u03b2, IL-8, and TNF-\u03b1 are upregulated whereas the counterinflammatory interleukine IL-10 is downregulated. The aim of this study was to identify in the long lasting intact ileoanal pouch cytokines which could have an protective effect or could lead to a new therapeutic option. MethodsSpecimens of each 10 were obtained endoscopically from the statuses: pre ileoanal pouch, pre closure of ileostomy, pouch 1 year in function, pouchitis, and controls. IL-6 and TNF-\u03b1 as proinflammatory and IL-10 as counter inflammatory cytokines were determined. Specimens were conserved at \u221280 \u00b0C. After PCR amplification the DNA concentration was measured and after further dilution the amplification served as a standard under the use of hybridisation probes. After RNA isolation and transcription into cDNA further quantification was performed against GAPDH via realtime-PCR. ResultsIL-6 was reduced in the status of the intact pouch 1 year compared to intact pouch Conclusion1. Upregulation of IL-6 or TNF-\u03b1 during pouchitis could not be observed. 2. Increased IL-10 in the mature pouch might be protective against pouchitis. 3. Topical IL-10 therapy could be a new issue for pouchitis treatment.", 
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    "name": "M\u00f6glichkeit der Pouchitis-Therapie durch Interleukin-10 im ileoanalen Pouch nach Colitis ulcerosa?", 
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