High-Resolution in Situ Confocal Analysis of Endothelial Cells View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2004

AUTHORS

Holger Gerhardt , Christer Betsholtz

ABSTRACT

The analysis of endothelial cells in situ differs in many ways from the study of cultured endothelium. Endothelial cells in situ are embedded in their natural environment — the tissue, which extends in three dimensions and exhibits some properties that are far from ideal for imaging techniques. These properties vary also within the organism. For example, the capillary plexus in the skin has a more planar dimension as compared to that of the lung, liver or brain. In general, the capillary plexus is patterned according to the dimensions and demands of the surrounding tissue. Consequently, the imaging techniques must be adapted to the three-dimensional properties of the tissue. Similarly, the optical properties differ between different tissues. Structures like hair, pigment, fat, or muscle fibers differ tremendously, for example, in transparency and autoflourescence. Accordingly, imaging techniques must be adapted to include bleaching of pigment or quenching of autoflourescence. In general, the maximum resolution will be achieved by imaging structures of minimal 3D dimensions and maximal transparency. Therefore, the choice of the tissue to be used as a model system for high-resolution analysis of endothelial cells is important and already sets the limits for the final resolution. Although physical sectioning increases the optical properties of a given tissue, it is often unwanted since it disrupts spatial relationships and, thus, limits overview and 3D reconstruction possibilities. More... »

PAGES

313-323

Book

TITLE

Methods in Endothelial Cell Biology

ISBN

978-3-540-21397-0
978-3-642-18725-4

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-18725-4_28

DOI

http://dx.doi.org/10.1007/978-3-642-18725-4_28

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011836771


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