NeoHepatocytes generated from patients with chronic liver disease can be used for autologous cell transplantation View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2009

AUTHORS

S. Ehnert , A. Lehmann , U. Böcker , S. Dooley , A. K. Nüssler

ABSTRACT

Liver transplantation is the only definitive treatment for end stage liver disease. Donor organ scarcity raises a growing interest in new therapeutic options. Recently, we have shown that injection of monocyte-derived Neo Hepatocytes can increase the survival in rats after extended liver resection. In order to apply this technology for autologous therapy, we generated NeoHepatocytes from patients with alcoholic liver disease and healthy controls and compared them to human hepatocytes. We obtained a comparable amount of monocytes from patients und controls from which we successfully generate Neo Hepatocytes. Although albumin expression remained very low, other hepatocyte markers, e.g. cytokeratin 18 and ADH1, increased significantly. Glucose and urea production was comparable with hepatocytes. Fat accumulation was induced by treatment with insulin, TGF-β and ethanol only in differentiated cells and hepatocytes. TGF-β signaling, critically required for progression of chronic liver disease, was comparable between Neo Hepatocytes and hepatocytes, merely expression of Smad1 and 3 was reduced (≈30/60%). Expression of TGF-β regulated genes, e.g. CTGF, fibronectin und collagen was lower in Neo Hepatocytes and we speculate that reduced expression of ECM can lead to a proliferation advantage after transplantation. Thus, this method might help patients bridge the waiting time for a suitable organ. More... »

PAGES

151-153

Book

TITLE

Chirurgisches Forum und DGAV Forum 2009

ISBN

978-3-642-00624-1
978-3-642-00625-8

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-642-00625-8_56

DOI

http://dx.doi.org/10.1007/978-3-642-00625-8_56

DIMENSIONS

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