Understanding the 3D architecture of organelle bound protein complexes using cryo-electron tomography of frozen hydrated sections and immunogold; our next ... View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2008

AUTHORS

Peter J. Peters , Jason Pierson , Diane Houben , Musa Sani , Sue Godsave , Pekka Kujala , Matthijn Vos , Erik Bos , Hans Jansen , Maaike van Zon , Nicole Van der Wel

ABSTRACT

One of the focal points of our group is to reveal the macromolecular organisation of cells by means of cryo-electron tomography. This is the only method that can be used to obtain molecular resolution information of intact cells constituencies in a near-native situation. The tomogram contains a 3D map of the cellular proteome and we are just beginning to explore its potential after we worked for a few years on some technical hurdles of specimen preparation. The other central point of our group is to localize gene product in cellular structures by EM at the highest resolution with gold probes on cryosections. Our main focus is on understanding the molecular machinery and organization within the endomembrane system. We concentrate our work on the transport mechanism within the endocytic pathway and the link to pathogenesis. Cryo-electron tomography of unfixed frozen hydrated cryo-sections and cryo immunogold-EM of aldehyde fixed cells are our main techniques, which allow the subcellular detection and visualization of molecular machines at the highest resolution. More... »

PAGES

339-340

Book

TITLE

EMC 2008 14th European Microscopy Congress 1–5 September 2008, Aachen, Germany

ISBN

978-3-540-85227-8
978-3-540-85228-5

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-540-85228-5_170

DOI

http://dx.doi.org/10.1007/978-3-540-85228-5_170

DIMENSIONS

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143 schema:name Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Plesmanlaan 121 - H4, 1066CX Amsterdam, The Netherlands
144 rdf:type schema:Organization
 




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