Various Approaches to the Microscopic Assessment of the Cornea, Visualization and Image Analysis of the Corneal Endothelium View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2017

AUTHORS

Katerina Jirsova , Jameson Clover , C.G. Stoeger , Gilles Thuret

ABSTRACT

This chapter describes the three main microscopic approaches used to assess corneal quality prior to grafting: slit lamp microscopy, transmitted light microscopy, and specular (i.e., reflection) microscopy. Slit lamp microscopy is preferably used to evaluate the cornea and the surrounding parts of the eye soon after enucleation or to assess a cornea placed in a viewing chamber during hypothermic storage. This approach allows signs of previous surgery, stromal opacities, epithelial defects, foreign bodies, or an abnormal corneal shape to be detected. Transmitted light microscopy is typically used to assess corneas during organ culture storage, while specular microscopy is generally used to evaluate corneas stored in hypothermic media. When an eye bank distributes corneas for different types of surgery (penetrating or lamellar keratoplasty), a careful examination helps to match the available tissue with the requirements of different surgical techniques and thus to optimize the utilization of a limited resource. Although all of the corneal layers, including the epithelium and the stroma, need to be evaluated if the cornea is intended for penetrating keratoplasty, the assessment is primarily focused on the corneal endothelium. The open system of organ culture allows the visualization of dead endothelial cells using trypan blue vital staining and of the cell borders using hypotonic solution. Recent technical progress allows the endothelium to be evaluated by various image analysis systems connected to user-friendly software programs that employ semiautomatic or fully automatic algorithms for cell assessment. More... »

PAGES

59-74

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-319-48845-5_4

DOI

http://dx.doi.org/10.1007/978-3-319-48845-5_4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1100714063


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1113", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Ophthalmology and Optometry", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Laboratory of the Biology and Pathology of the Eye, Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University, Ke Karlovu 2, 128 08, Prague 2, Czech Republic", 
          "id": "http://www.grid.ac/institutes/grid.4491.8", 
          "name": [
            "Laboratory of the Biology and Pathology of the Eye, Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University, Ke Karlovu 2, 128 08, Prague 2, Czech Republic"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jirsova", 
        "givenName": "Katerina", 
        "id": "sg:person.01360341723.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01360341723.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Lions VisionGift, 2201 SE 11th Ave., 97214, Portland, OR, USA", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Lions VisionGift, 2201 SE 11th Ave., 97214, Portland, OR, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Clover", 
        "givenName": "Jameson", 
        "id": "sg:person.0760305711.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760305711.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Lions VisionGift, 2201 SE 11th Ave., 97214, Portland, OR, USA", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Lions VisionGift, 2201 SE 11th Ave., 97214, Portland, OR, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Stoeger", 
        "givenName": "C.G.", 
        "id": "sg:person.0752623305.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752623305.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Corneal Graft Biology, Engineering, and Imaging Laboratory, Jean Monnet University, 10, rue de la Marandi\u00e8re, 42055, Saint-Etienne, France", 
          "id": "http://www.grid.ac/institutes/grid.6279.a", 
          "name": [
            "Corneal Graft Biology, Engineering, and Imaging Laboratory, Jean Monnet University, 10, rue de la Marandi\u00e8re, 42055, Saint-Etienne, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thuret", 
        "givenName": "Gilles", 
        "id": "sg:person.01366453064.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366453064.75"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2017", 
    "datePublishedReg": "2017-01-01", 
    "description": "This chapter describes the three main microscopic approaches used to assess corneal quality prior to grafting: slit lamp microscopy, transmitted light microscopy, and specular (i.e., reflection) microscopy. Slit lamp microscopy is preferably used to evaluate the cornea and the surrounding parts of the eye soon after enucleation or to assess a cornea placed in a viewing chamber during hypothermic storage. This approach allows signs of previous surgery, stromal opacities, epithelial defects, foreign bodies, or an abnormal corneal shape to be detected. Transmitted light microscopy is typically used to assess corneas during organ culture storage, while specular microscopy is generally used to evaluate corneas stored in hypothermic media. When an eye bank distributes corneas for different types of surgery (penetrating or lamellar keratoplasty), a careful examination helps to match the available tissue with the requirements of different surgical techniques and thus to optimize the utilization of a limited resource. Although all of the corneal layers, including the epithelium and the stroma, need to be evaluated if the cornea is intended for penetrating keratoplasty, the assessment is primarily focused on the corneal endothelium. The open system of organ culture allows the visualization of dead endothelial cells using trypan blue vital staining and of the cell borders using hypotonic solution. Recent technical progress allows the endothelium to be evaluated by various image analysis systems connected to user-friendly software programs that employ semiautomatic or fully automatic algorithms for cell assessment.", 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-3-319-48845-5_4", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-3-319-48843-1", 
        "978-3-319-48845-5"
      ], 
      "name": "Light and Specular Microscopy of the Cornea", 
      "type": "Book"
    }, 
    "keywords": [
      "lamp microscopy", 
      "specular microscopy", 
      "corneal endothelium", 
      "abnormal corneal shape", 
      "slit-lamp microscopy", 
      "different surgical techniques", 
      "trypan blue vital staining", 
      "hypothermic medium", 
      "previous surgery", 
      "stromal opacity", 
      "blue vital staining", 
      "epithelial defects", 
      "surgical technique", 
      "foreign body", 
      "eye bank", 
      "dead endothelial cells", 
      "corneal layers", 
      "light microscopy", 
      "available tissue", 
      "corneal quality", 
      "endothelial cells", 
      "cornea", 
      "corneal shape", 
      "cell assessment", 
      "endothelium", 
      "microscopic assessment", 
      "organ culture", 
      "surgery", 
      "hypothermic storage", 
      "hypotonic solution", 
      "vital staining", 
      "image analysis system", 
      "cell borders", 
      "organ culture storage", 
      "assessment", 
      "keratoplasty", 
      "enucleation", 
      "careful examination", 
      "stroma", 
      "epithelium", 
      "staining", 
      "eyes", 
      "tissue", 
      "signs", 
      "examination", 
      "recent technical progress", 
      "cells", 
      "opacity", 
      "analysis system", 
      "limited resources", 
      "user-friendly software program", 
      "software program", 
      "transmitted light microscopy", 
      "defects", 
      "image analysis", 
      "culture storage", 
      "visualization", 
      "different types", 
      "body", 
      "culture", 
      "program", 
      "quality", 
      "microscopy", 
      "border", 
      "types", 
      "utilization", 
      "analysis", 
      "approach", 
      "progress", 
      "chamber", 
      "part", 
      "system", 
      "microscopic approach", 
      "technique", 
      "automatic algorithm", 
      "medium", 
      "chapter", 
      "storage", 
      "requirements", 
      "resources", 
      "technical progress", 
      "layer", 
      "shape", 
      "banks", 
      "solution", 
      "algorithm", 
      "open system"
    ], 
    "name": "Various Approaches to the Microscopic Assessment of the Cornea, Visualization and Image Analysis of the Corneal Endothelium", 
    "pagination": "59-74", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1100714063"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-3-319-48845-5_4"
        ]
      }
    ], 
    "publisher": {
      "name": "Springer Nature", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-3-319-48845-5_4", 
      "https://app.dimensions.ai/details/publication/pub.1100714063"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2022-05-10T10:53", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220509/entities/gbq_results/chapter/chapter_445.jsonl", 
    "type": "Chapter", 
    "url": "https://doi.org/10.1007/978-3-319-48845-5_4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-3-319-48845-5_4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-3-319-48845-5_4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-3-319-48845-5_4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-3-319-48845-5_4'


 

This table displays all metadata directly associated to this object as RDF triples.

168 TRIPLES      22 PREDICATES      111 URIs      104 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-3-319-48845-5_4 schema:about anzsrc-for:11
2 anzsrc-for:1113
3 schema:author N2f77fc3f3bf146a0b0916bd319e2b03c
4 schema:datePublished 2017
5 schema:datePublishedReg 2017-01-01
6 schema:description This chapter describes the three main microscopic approaches used to assess corneal quality prior to grafting: slit lamp microscopy, transmitted light microscopy, and specular (i.e., reflection) microscopy. Slit lamp microscopy is preferably used to evaluate the cornea and the surrounding parts of the eye soon after enucleation or to assess a cornea placed in a viewing chamber during hypothermic storage. This approach allows signs of previous surgery, stromal opacities, epithelial defects, foreign bodies, or an abnormal corneal shape to be detected. Transmitted light microscopy is typically used to assess corneas during organ culture storage, while specular microscopy is generally used to evaluate corneas stored in hypothermic media. When an eye bank distributes corneas for different types of surgery (penetrating or lamellar keratoplasty), a careful examination helps to match the available tissue with the requirements of different surgical techniques and thus to optimize the utilization of a limited resource. Although all of the corneal layers, including the epithelium and the stroma, need to be evaluated if the cornea is intended for penetrating keratoplasty, the assessment is primarily focused on the corneal endothelium. The open system of organ culture allows the visualization of dead endothelial cells using trypan blue vital staining and of the cell borders using hypotonic solution. Recent technical progress allows the endothelium to be evaluated by various image analysis systems connected to user-friendly software programs that employ semiautomatic or fully automatic algorithms for cell assessment.
7 schema:genre chapter
8 schema:inLanguage en
9 schema:isAccessibleForFree false
10 schema:isPartOf N820020f937ce45e9b5de54d226e02f40
11 schema:keywords abnormal corneal shape
12 algorithm
13 analysis
14 analysis system
15 approach
16 assessment
17 automatic algorithm
18 available tissue
19 banks
20 blue vital staining
21 body
22 border
23 careful examination
24 cell assessment
25 cell borders
26 cells
27 chamber
28 chapter
29 cornea
30 corneal endothelium
31 corneal layers
32 corneal quality
33 corneal shape
34 culture
35 culture storage
36 dead endothelial cells
37 defects
38 different surgical techniques
39 different types
40 endothelial cells
41 endothelium
42 enucleation
43 epithelial defects
44 epithelium
45 examination
46 eye bank
47 eyes
48 foreign body
49 hypothermic medium
50 hypothermic storage
51 hypotonic solution
52 image analysis
53 image analysis system
54 keratoplasty
55 lamp microscopy
56 layer
57 light microscopy
58 limited resources
59 medium
60 microscopic approach
61 microscopic assessment
62 microscopy
63 opacity
64 open system
65 organ culture
66 organ culture storage
67 part
68 previous surgery
69 program
70 progress
71 quality
72 recent technical progress
73 requirements
74 resources
75 shape
76 signs
77 slit-lamp microscopy
78 software program
79 solution
80 specular microscopy
81 staining
82 storage
83 stroma
84 stromal opacity
85 surgery
86 surgical technique
87 system
88 technical progress
89 technique
90 tissue
91 transmitted light microscopy
92 trypan blue vital staining
93 types
94 user-friendly software program
95 utilization
96 visualization
97 vital staining
98 schema:name Various Approaches to the Microscopic Assessment of the Cornea, Visualization and Image Analysis of the Corneal Endothelium
99 schema:pagination 59-74
100 schema:productId N0e33844fa4c942a1acb899923cc22fba
101 Nac5b7ee962824d7181c15d66e4fa7742
102 schema:publisher N8a03ab6ee6b24dbcb29144afb4f11cc1
103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1100714063
104 https://doi.org/10.1007/978-3-319-48845-5_4
105 schema:sdDatePublished 2022-05-10T10:53
106 schema:sdLicense https://scigraph.springernature.com/explorer/license/
107 schema:sdPublisher Nde4b53c89dc1477a8dd8175ec19138d7
108 schema:url https://doi.org/10.1007/978-3-319-48845-5_4
109 sgo:license sg:explorer/license/
110 sgo:sdDataset chapters
111 rdf:type schema:Chapter
112 N045964e8267349a4a30b5972899ea445 rdf:first sg:person.01366453064.75
113 rdf:rest rdf:nil
114 N0e33844fa4c942a1acb899923cc22fba schema:name dimensions_id
115 schema:value pub.1100714063
116 rdf:type schema:PropertyValue
117 N2f77fc3f3bf146a0b0916bd319e2b03c rdf:first sg:person.01360341723.02
118 rdf:rest Nea00aea11302442285b216945f5249f9
119 N3f947f5e800a440d9be771cabba7f9e1 rdf:first sg:person.0752623305.20
120 rdf:rest N045964e8267349a4a30b5972899ea445
121 N820020f937ce45e9b5de54d226e02f40 schema:isbn 978-3-319-48843-1
122 978-3-319-48845-5
123 schema:name Light and Specular Microscopy of the Cornea
124 rdf:type schema:Book
125 N8a03ab6ee6b24dbcb29144afb4f11cc1 schema:name Springer Nature
126 rdf:type schema:Organisation
127 Nac5b7ee962824d7181c15d66e4fa7742 schema:name doi
128 schema:value 10.1007/978-3-319-48845-5_4
129 rdf:type schema:PropertyValue
130 Nde4b53c89dc1477a8dd8175ec19138d7 schema:name Springer Nature - SN SciGraph project
131 rdf:type schema:Organization
132 Nea00aea11302442285b216945f5249f9 rdf:first sg:person.0760305711.57
133 rdf:rest N3f947f5e800a440d9be771cabba7f9e1
134 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
135 schema:name Medical and Health Sciences
136 rdf:type schema:DefinedTerm
137 anzsrc-for:1113 schema:inDefinedTermSet anzsrc-for:
138 schema:name Ophthalmology and Optometry
139 rdf:type schema:DefinedTerm
140 sg:person.01360341723.02 schema:affiliation grid-institutes:grid.4491.8
141 schema:familyName Jirsova
142 schema:givenName Katerina
143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01360341723.02
144 rdf:type schema:Person
145 sg:person.01366453064.75 schema:affiliation grid-institutes:grid.6279.a
146 schema:familyName Thuret
147 schema:givenName Gilles
148 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366453064.75
149 rdf:type schema:Person
150 sg:person.0752623305.20 schema:affiliation grid-institutes:None
151 schema:familyName Stoeger
152 schema:givenName C.G.
153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752623305.20
154 rdf:type schema:Person
155 sg:person.0760305711.57 schema:affiliation grid-institutes:None
156 schema:familyName Clover
157 schema:givenName Jameson
158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0760305711.57
159 rdf:type schema:Person
160 grid-institutes:None schema:alternateName Lions VisionGift, 2201 SE 11th Ave., 97214, Portland, OR, USA
161 schema:name Lions VisionGift, 2201 SE 11th Ave., 97214, Portland, OR, USA
162 rdf:type schema:Organization
163 grid-institutes:grid.4491.8 schema:alternateName Laboratory of the Biology and Pathology of the Eye, Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University, Ke Karlovu 2, 128 08, Prague 2, Czech Republic
164 schema:name Laboratory of the Biology and Pathology of the Eye, Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University, Ke Karlovu 2, 128 08, Prague 2, Czech Republic
165 rdf:type schema:Organization
166 grid-institutes:grid.6279.a schema:alternateName Corneal Graft Biology, Engineering, and Imaging Laboratory, Jean Monnet University, 10, rue de la Marandière, 42055, Saint-Etienne, France
167 schema:name Corneal Graft Biology, Engineering, and Imaging Laboratory, Jean Monnet University, 10, rue de la Marandière, 42055, Saint-Etienne, France
168 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...