Nuclear Magnetic Resonance Strategies for Metabolic Analysis View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2017-01-29

AUTHORS

Clement Heude , Jay Nath , John Bosco Carrigan , Christian Ludwig

ABSTRACT

NMR spectroscopy is a powerful tool for metabolomic studies, offering highly reproducible and quantitative analyses. This burgeoning field of NMR metabolomics has been greatly aided by the development of modern spectrometers and software, allowing high-throughput analysis with near real-time feedback. Whilst one-dimensional proton (1D-1H) NMR analysis is best described and remains most widely used, a plethora of alternative NMR techniques are now available that offer additional chemical and structural information and resolve many of the limitations of conventional 1D-1H NMR such as spectral overlay. In this book chapter, we review the principal concepts of practical NMR spectroscopy, from common sample preparation protocols to the benefits and theoretical concepts underpinning the commonly used pulse sequences. Finally, as a case study to highlight the utility of NMR as a method for metabolomic investigation, we have detailed how NMR has been used to gain valuable insight into the metabolism occurring in kidneys prior to transplantation and the potential implications of this. More... »

PAGES

45-76

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-319-47656-8_3

DOI

http://dx.doi.org/10.1007/978-3-319-47656-8_3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1074241898

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28132176


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomarkers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Magnetic Resonance Spectroscopy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Metabolic Networks and Pathways", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Metabolomics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Organ Preservation", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institute of Metabolism and Systems Research (IMSR), University of Birmingham, Birmingham, UK", 
          "id": "http://www.grid.ac/institutes/grid.6572.6", 
          "name": [
            "NERC Metabolomics Facility, School of Biosciences, University of Birmingham, Birmingham, UK", 
            "Institute of Metabolism and Systems Research (IMSR), University of Birmingham, Birmingham, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Heude", 
        "givenName": "Clement", 
        "id": "sg:person.015521761567.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015521761567.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, B152TT, Birmingham, UK", 
          "id": "http://www.grid.ac/institutes/grid.6572.6", 
          "name": [
            "Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, B152TT, Birmingham, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nath", 
        "givenName": "Jay", 
        "id": "sg:person.01147135474.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147135474.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, B152TT, Birmingham, UK", 
          "id": "http://www.grid.ac/institutes/grid.6572.6", 
          "name": [
            "Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, B152TT, Birmingham, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Carrigan", 
        "givenName": "John Bosco", 
        "id": "sg:person.0671673626.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0671673626.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, B152TT, Birmingham, UK", 
          "id": "http://www.grid.ac/institutes/grid.6572.6", 
          "name": [
            "Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, B152TT, Birmingham, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ludwig", 
        "givenName": "Christian", 
        "id": "sg:person.01112544071.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01112544071.19"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2017-01-29", 
    "datePublishedReg": "2017-01-29", 
    "description": "NMR spectroscopy is a powerful tool for metabolomic studies, offering highly reproducible and quantitative analyses. This burgeoning field of NMR metabolomics has been greatly aided by the development of modern spectrometers and software, allowing high-throughput analysis with near real-time feedback. Whilst one-dimensional proton (1D-1H) NMR analysis is best described and remains most widely used, a plethora of alternative NMR techniques are now available that offer additional chemical and structural information and resolve many of the limitations of conventional 1D-1H NMR such as spectral overlay. In this book chapter, we review the principal concepts of practical NMR spectroscopy, from common sample preparation protocols to the benefits and theoretical concepts underpinning the commonly used pulse sequences. Finally, as a case study to highlight the utility of NMR as a method for metabolomic investigation, we have detailed how NMR has been used to gain valuable insight into the metabolism occurring in kidneys prior to transplantation and the potential implications of this.", 
    "editor": [
      {
        "familyName": "Sussulini", 
        "givenName": "Alessandra", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-3-319-47656-8_3", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-3-319-47655-1", 
        "978-3-319-47656-8"
      ], 
      "name": "Metabolomics: From Fundamentals to Clinical Applications", 
      "type": "Book"
    }, 
    "keywords": [
      "NMR spectroscopy", 
      "nuclear magnetic resonance (NMR) strategy", 
      "sample preparation protocol", 
      "utility of NMR", 
      "proton NMR analysis", 
      "NMR techniques", 
      "NMR analysis", 
      "NMR", 
      "additional chemicals", 
      "high-throughput analysis", 
      "preparation protocol", 
      "structural information", 
      "resonance strategies", 
      "spectroscopy", 
      "modern spectrometers", 
      "spectral overlay", 
      "NMR metabolomics", 
      "metabolomics studies", 
      "metabolomic investigation", 
      "quantitative analysis", 
      "spectrometer", 
      "powerful tool", 
      "chemicals", 
      "pulse sequence", 
      "book chapter", 
      "metabolomics", 
      "valuable insights", 
      "metabolic analysis", 
      "analysis", 
      "investigation", 
      "technique", 
      "method", 
      "insights", 
      "plethora", 
      "study", 
      "field", 
      "chapter", 
      "principal concepts", 
      "utility", 
      "theoretical concepts", 
      "strategies", 
      "protocol", 
      "limitations", 
      "development", 
      "tool", 
      "potential implications", 
      "real-time feedback", 
      "concept", 
      "sequence", 
      "information", 
      "metabolism", 
      "overlay", 
      "implications", 
      "case study", 
      "feedback", 
      "software", 
      "benefits", 
      "kidney", 
      "transplantation", 
      "one-dimensional proton (1D-1H) NMR analysis", 
      "alternative NMR techniques", 
      "practical NMR spectroscopy", 
      "common sample preparation protocols", 
      "Magnetic Resonance Strategies"
    ], 
    "name": "Nuclear Magnetic Resonance Strategies for Metabolic Analysis", 
    "pagination": "45-76", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1074241898"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-3-319-47656-8_3"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28132176"
        ]
      }
    ], 
    "publisher": {
      "name": "Springer Nature", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-3-319-47656-8_3", 
      "https://app.dimensions.ai/details/publication/pub.1074241898"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2021-12-01T20:09", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/chapter/chapter_412.jsonl", 
    "type": "Chapter", 
    "url": "https://doi.org/10.1007/978-3-319-47656-8_3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-3-319-47656-8_3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-3-319-47656-8_3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-3-319-47656-8_3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-3-319-47656-8_3'


 

This table displays all metadata directly associated to this object as RDF triples.

172 TRIPLES      23 PREDICATES      94 URIs      88 LITERALS      14 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-3-319-47656-8_3 schema:about N0baa250f554741499153bb043873ab41
2 N3dc62f84a48d411da711daf3ab91bebb
3 N3f130ad4c3994e42b67815beb98f6cae
4 Na642b1d7e8e54a108837d1a526c4de00
5 Nced5696d8dae4d648b05ceb9bc7939d7
6 Nf271c6e4787a401394c43c5a44dcd951
7 anzsrc-for:11
8 schema:author N3e343a19e77e47e5a0638c682fff93bb
9 schema:datePublished 2017-01-29
10 schema:datePublishedReg 2017-01-29
11 schema:description NMR spectroscopy is a powerful tool for metabolomic studies, offering highly reproducible and quantitative analyses. This burgeoning field of NMR metabolomics has been greatly aided by the development of modern spectrometers and software, allowing high-throughput analysis with near real-time feedback. Whilst one-dimensional proton (1D-1H) NMR analysis is best described and remains most widely used, a plethora of alternative NMR techniques are now available that offer additional chemical and structural information and resolve many of the limitations of conventional 1D-1H NMR such as spectral overlay. In this book chapter, we review the principal concepts of practical NMR spectroscopy, from common sample preparation protocols to the benefits and theoretical concepts underpinning the commonly used pulse sequences. Finally, as a case study to highlight the utility of NMR as a method for metabolomic investigation, we have detailed how NMR has been used to gain valuable insight into the metabolism occurring in kidneys prior to transplantation and the potential implications of this.
12 schema:editor N1b53db2408e6441b83eba2c4f4ffcaa2
13 schema:genre chapter
14 schema:inLanguage en
15 schema:isAccessibleForFree false
16 schema:isPartOf Nece92711beed4f0ead6bcda785a56f7f
17 schema:keywords Magnetic Resonance Strategies
18 NMR
19 NMR analysis
20 NMR metabolomics
21 NMR spectroscopy
22 NMR techniques
23 additional chemicals
24 alternative NMR techniques
25 analysis
26 benefits
27 book chapter
28 case study
29 chapter
30 chemicals
31 common sample preparation protocols
32 concept
33 development
34 feedback
35 field
36 high-throughput analysis
37 implications
38 information
39 insights
40 investigation
41 kidney
42 limitations
43 metabolic analysis
44 metabolism
45 metabolomic investigation
46 metabolomics
47 metabolomics studies
48 method
49 modern spectrometers
50 nuclear magnetic resonance (NMR) strategy
51 one-dimensional proton (1D-1H) NMR analysis
52 overlay
53 plethora
54 potential implications
55 powerful tool
56 practical NMR spectroscopy
57 preparation protocol
58 principal concepts
59 protocol
60 proton NMR analysis
61 pulse sequence
62 quantitative analysis
63 real-time feedback
64 resonance strategies
65 sample preparation protocol
66 sequence
67 software
68 spectral overlay
69 spectrometer
70 spectroscopy
71 strategies
72 structural information
73 study
74 technique
75 theoretical concepts
76 tool
77 transplantation
78 utility
79 utility of NMR
80 valuable insights
81 schema:name Nuclear Magnetic Resonance Strategies for Metabolic Analysis
82 schema:pagination 45-76
83 schema:productId N34964dc06ff54f64a053178e047eae1d
84 Na701127bd80d4f1385b3d5ba5b7c28ad
85 Nb559594cd3fc419ead364e6c79129aff
86 schema:publisher Ne0534f5f19174cc6aaceb527d12fa617
87 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074241898
88 https://doi.org/10.1007/978-3-319-47656-8_3
89 schema:sdDatePublished 2021-12-01T20:09
90 schema:sdLicense https://scigraph.springernature.com/explorer/license/
91 schema:sdPublisher N523f3783547d404baa1a7d54d6954af5
92 schema:url https://doi.org/10.1007/978-3-319-47656-8_3
93 sgo:license sg:explorer/license/
94 sgo:sdDataset chapters
95 rdf:type schema:Chapter
96 N0baa250f554741499153bb043873ab41 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Metabolomics
98 rdf:type schema:DefinedTerm
99 N1b53db2408e6441b83eba2c4f4ffcaa2 rdf:first Nc0bbe8ae81954661b53237f87ff3a7ca
100 rdf:rest rdf:nil
101 N34964dc06ff54f64a053178e047eae1d schema:name pubmed_id
102 schema:value 28132176
103 rdf:type schema:PropertyValue
104 N3dc62f84a48d411da711daf3ab91bebb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Humans
106 rdf:type schema:DefinedTerm
107 N3e343a19e77e47e5a0638c682fff93bb rdf:first sg:person.015521761567.55
108 rdf:rest Nebf75a941e6d4c1fa21798d1088517ff
109 N3f130ad4c3994e42b67815beb98f6cae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Organ Preservation
111 rdf:type schema:DefinedTerm
112 N43c65bb40e924686a6710ef6ea580ba9 rdf:first sg:person.01112544071.19
113 rdf:rest rdf:nil
114 N523f3783547d404baa1a7d54d6954af5 schema:name Springer Nature - SN SciGraph project
115 rdf:type schema:Organization
116 N552f62a6d0814463af1bd08ca4922349 rdf:first sg:person.0671673626.85
117 rdf:rest N43c65bb40e924686a6710ef6ea580ba9
118 Na642b1d7e8e54a108837d1a526c4de00 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Metabolic Networks and Pathways
120 rdf:type schema:DefinedTerm
121 Na701127bd80d4f1385b3d5ba5b7c28ad schema:name dimensions_id
122 schema:value pub.1074241898
123 rdf:type schema:PropertyValue
124 Nb559594cd3fc419ead364e6c79129aff schema:name doi
125 schema:value 10.1007/978-3-319-47656-8_3
126 rdf:type schema:PropertyValue
127 Nc0bbe8ae81954661b53237f87ff3a7ca schema:familyName Sussulini
128 schema:givenName Alessandra
129 rdf:type schema:Person
130 Nced5696d8dae4d648b05ceb9bc7939d7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Biomarkers
132 rdf:type schema:DefinedTerm
133 Ne0534f5f19174cc6aaceb527d12fa617 schema:name Springer Nature
134 rdf:type schema:Organisation
135 Nebf75a941e6d4c1fa21798d1088517ff rdf:first sg:person.01147135474.12
136 rdf:rest N552f62a6d0814463af1bd08ca4922349
137 Nece92711beed4f0ead6bcda785a56f7f schema:isbn 978-3-319-47655-1
138 978-3-319-47656-8
139 schema:name Metabolomics: From Fundamentals to Clinical Applications
140 rdf:type schema:Book
141 Nf271c6e4787a401394c43c5a44dcd951 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
142 schema:name Magnetic Resonance Spectroscopy
143 rdf:type schema:DefinedTerm
144 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
145 schema:name Medical and Health Sciences
146 rdf:type schema:DefinedTerm
147 sg:person.01112544071.19 schema:affiliation grid-institutes:grid.6572.6
148 schema:familyName Ludwig
149 schema:givenName Christian
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01112544071.19
151 rdf:type schema:Person
152 sg:person.01147135474.12 schema:affiliation grid-institutes:grid.6572.6
153 schema:familyName Nath
154 schema:givenName Jay
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01147135474.12
156 rdf:type schema:Person
157 sg:person.015521761567.55 schema:affiliation grid-institutes:grid.6572.6
158 schema:familyName Heude
159 schema:givenName Clement
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015521761567.55
161 rdf:type schema:Person
162 sg:person.0671673626.85 schema:affiliation grid-institutes:grid.6572.6
163 schema:familyName Carrigan
164 schema:givenName John Bosco
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0671673626.85
166 rdf:type schema:Person
167 grid-institutes:grid.6572.6 schema:alternateName Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, B152TT, Birmingham, UK
168 Institute of Metabolism and Systems Research (IMSR), University of Birmingham, Birmingham, UK
169 schema:name Institute of Metabolism and Systems Research (IMSR), College of Medical and Dental Sciences, University of Birmingham, B152TT, Birmingham, UK
170 Institute of Metabolism and Systems Research (IMSR), University of Birmingham, Birmingham, UK
171 NERC Metabolomics Facility, School of Biosciences, University of Birmingham, Birmingham, UK
172 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...