Functional Analysis and the Role of Members of SGT Gene Family of Withania somnifera View Full Text


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Chapter Info

DATE

2017-05-09

AUTHORS

Pratibha Misra , Gaurav Singh , Manoj K. Mishra , Vibha Pandey , Syed Saema

ABSTRACT

Sterol glycosyltransferases (SGTs) catalyze the attachment of a carbohydrate moiety to an aglycone sterol accepter molecule at different positions. SGTs are key enzymes for the biosynthesis of many precious natural plant products. SGTs of Withania somnifera (WsSGTs) help in the glycosylation of withanolides, a pharmaceutically important C-28 phytochemical product and phytosterols, such as sitosterol and stigmasterol. SGTs of W. somnifera glycosylate the sterol backbone at C-3, C-17, and C-27 positions. Modified phytosterols and withanolides play an important role in maintaining metabolic plasticity during adaptive response. The expression of SGTs changed during different biotic and abiotic stresses indicating their role in maintaining the cellular disturbances. Overexpression of WsSGTL1, a gene member of SGT gene family and silencing of SGT members through RNAi and artificial miRNA technology, in homologous (W. somnifera) and heterologous (Nicotiana tabacum and Arabidopsis thaliana) expression systems defines their role in growth and development of plants. The functional analysis of these genes has also been studied under abiotic (cold, heat, and salt) and biotic (SA, JA, Alternaria alternata, and Spodoptera litura) stress-providing tolerance to the plants. The chapter is concerned with the importance and application of SGTs in metabolic pathway engineering leading to biosynthesis of important bioactive compounds in W. somnifera. More... »

PAGES

539-552

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-319-28669-3_16

DOI

http://dx.doi.org/10.1007/978-3-319-28669-3_16

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