Collecting and Processing Wood Microcores for Monitoring Xylogenesis View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2015-09-20

AUTHORS

Annie Deslauriers , Sergio Rossi , Eryuan Liang

ABSTRACT

Detailed analyses of cambium activity and wood formation need repeated samplings of the developing xylem and specific procedures for sample preparation. In order to minimize the damage to the stem, wood samples are extracted as microcores. Because of the small size of the samples, inclusion in an embedding medium, such as paraffin, is suggested to adequately fix the samples and to cut the tissues in thin sections with a microtome. Sections are stained and observed under normal and polarized light to identify the cambial region, stages of wood formation (enlargement and cell wall thickening) and mature cells. This procedure allows quantitative data to be obtained quickly to reconstruct timings and patterns of wood formation (duration and rates) in tree species. More... »

PAGES

417-429

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-319-19944-3_23

DOI

http://dx.doi.org/10.1007/978-3-319-19944-3_23

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1012957506


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