Chimerism Testing in Allogeneic Hematopoietic Stem Cell Transplantation View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2016

AUTHORS

Vivianna M. Van Deerlin , Ran Reshef

ABSTRACT

Allogeneic hematopoietic stem cell transplantation (HSCT) is a well-established treatment option for a variety of malignant and nonmalignant diseases. Each year, more than 28,000 patients undergo allogeneic HSCT worldwide. Molecular analysis of chimerism levels is used to monitor the proportions of donor and recipient hematopoietic cells in patients after HSCT. Chimerism studies can help identify the source of hematopoiesis after engraftment, identify graft rejection in patients with poor hematologic recovery, and assist in the diagnosis and prediction of relapse and clinical graft-vs-host disease (GVHD). The most commonly applied method is PCR amplification of short tandem repeats (STR), a type of genetic polymorphism. The interpretation of chimerism results depends on the context in which the testing was done, i.e., the type of transplant, conditioning intensity, timing of testing post-transplant and results of concurrent blood counts and bone marrow morphology. While the methodology is technically straightforward, the implementation, analysis, and interpretation of results can be complicated. The clinical importance of chimerism results warrants the effort required by the laboratory for validation and performance of the test. In this chapter we review the clinical utility of chimerism analysis and laboratory aspects of testing, including methods, reagents, approaches to pre- and post-transplant testing, analysis and interpretation, pitfalls and limitations, reporting, and data management. More... »

PAGES

823-848

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-319-19674-9_56

DOI

http://dx.doi.org/10.1007/978-3-319-19674-9_56

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019835104


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Clinical Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Pathology and Laboratory Medicine, Molecular Pathology Laboratory, Perelman School of Medicine at the University of Pennsylvania, 3400 Spruce St., 7.103 Founders Pavilion, 19104, Philadelphia, PA, USA", 
          "id": "http://www.grid.ac/institutes/grid.25879.31", 
          "name": [
            "Pathology and Laboratory Medicine, Molecular Pathology Laboratory, Perelman School of Medicine at the University of Pennsylvania, 3400 Spruce St., 7.103 Founders Pavilion, 19104, Philadelphia, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Van Deerlin", 
        "givenName": "Vivianna M.", 
        "id": "sg:person.014435572617.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014435572617.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Medicine, Hematology & Oncology Division, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA", 
          "id": "http://www.grid.ac/institutes/grid.25879.31", 
          "name": [
            "Department of Medicine, Hematology & Oncology Division, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Reshef", 
        "givenName": "Ran", 
        "id": "sg:person.01267452463.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01267452463.62"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2016", 
    "datePublishedReg": "2016-01-01", 
    "description": "Allogeneic hematopoietic stem cell transplantation (HSCT) is a well-established treatment option for a variety of malignant and nonmalignant diseases. Each year, more than 28,000 patients undergo allogeneic HSCT worldwide. Molecular analysis of chimerism levels is used to monitor the proportions of donor and recipient hematopoietic cells in patients after HSCT. Chimerism studies can help identify the source of hematopoiesis after engraftment, identify graft rejection in patients with poor hematologic recovery, and assist in the diagnosis and prediction of relapse and clinical graft-vs-host disease (GVHD). The most commonly applied method is PCR amplification of short tandem repeats (STR), a type of genetic polymorphism. The interpretation of chimerism results depends on the context in which the testing was done, i.e., the type of transplant, conditioning intensity, timing of testing post-transplant and results of concurrent blood counts and bone marrow morphology. While the methodology is technically straightforward, the implementation, analysis, and interpretation of results can be complicated. The clinical importance of chimerism results warrants the effort required by the laboratory for validation and performance of the test. In this chapter we review the clinical utility of chimerism analysis and laboratory aspects of testing, including methods, reagents, approaches to pre- and post-transplant testing, analysis and interpretation, pitfalls and limitations, reporting, and data management.", 
    "editor": [
      {
        "familyName": "Leonard", 
        "givenName": "Debra G.B.", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-3-319-19674-9_56", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-3-319-19673-2", 
        "978-3-319-19674-9"
      ], 
      "name": "Molecular Pathology in Clinical Practice", 
      "type": "Book"
    }, 
    "keywords": [
      "hematopoietic stem cell transplantation", 
      "allogeneic hematopoietic stem cell transplantation", 
      "stem cell transplantation", 
      "cell transplantation", 
      "chimerism results", 
      "type of transplant", 
      "bone marrow morphology", 
      "proportion of donors", 
      "prediction of relapse", 
      "host disease", 
      "graft rejection", 
      "hematologic recovery", 
      "clinical graft", 
      "nonmalignant diseases", 
      "chimerism levels", 
      "conditioning intensity", 
      "blood count", 
      "treatment options", 
      "chimerism studies", 
      "marrow morphology", 
      "laboratory aspects", 
      "clinical utility", 
      "clinical importance", 
      "chimerism analysis", 
      "patients", 
      "genetic polymorphisms", 
      "chimerism testing", 
      "transplantation", 
      "disease", 
      "hematopoietic cells", 
      "interpretation of results", 
      "relapse", 
      "transplant", 
      "graft", 
      "engraftment", 
      "molecular analysis", 
      "testing", 
      "diagnosis", 
      "PCR amplification", 
      "counts", 
      "hematopoiesis", 
      "polymorphism", 
      "cells", 
      "options", 
      "years", 
      "short tandem repeats", 
      "donors", 
      "rejection", 
      "proportion", 
      "reporting", 
      "management", 
      "levels", 
      "recovery", 
      "results", 
      "analysis", 
      "study", 
      "types", 
      "pitfalls", 
      "test", 
      "timing", 
      "tandem repeats", 
      "utility", 
      "amplification", 
      "laboratory", 
      "importance", 
      "method", 
      "validation", 
      "variety", 
      "limitations", 
      "repeats", 
      "interpretation", 
      "aspects", 
      "efforts", 
      "intensity", 
      "morphology", 
      "reagents", 
      "chapter", 
      "source", 
      "context", 
      "implementation", 
      "prediction", 
      "methodology", 
      "performance", 
      "data management", 
      "source of hematopoiesis", 
      "poor hematologic recovery", 
      "concurrent blood counts", 
      "post-transplant testing"
    ], 
    "name": "Chimerism Testing in Allogeneic Hematopoietic Stem Cell Transplantation", 
    "pagination": "823-848", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1019835104"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-3-319-19674-9_56"
        ]
      }
    ], 
    "publisher": {
      "name": "Springer Nature", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-3-319-19674-9_56", 
      "https://app.dimensions.ai/details/publication/pub.1019835104"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2021-11-01T18:54", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211101/entities/gbq_results/chapter/chapter_302.jsonl", 
    "type": "Chapter", 
    "url": "https://doi.org/10.1007/978-3-319-19674-9_56"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-3-319-19674-9_56'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-3-319-19674-9_56'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-3-319-19674-9_56'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-3-319-19674-9_56'


 

This table displays all metadata directly associated to this object as RDF triples.

157 TRIPLES      23 PREDICATES      113 URIs      106 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-3-319-19674-9_56 schema:about anzsrc-for:11
2 anzsrc-for:1103
3 schema:author N62aef916d9dc4634a24cbd60e54bdaee
4 schema:datePublished 2016
5 schema:datePublishedReg 2016-01-01
6 schema:description Allogeneic hematopoietic stem cell transplantation (HSCT) is a well-established treatment option for a variety of malignant and nonmalignant diseases. Each year, more than 28,000 patients undergo allogeneic HSCT worldwide. Molecular analysis of chimerism levels is used to monitor the proportions of donor and recipient hematopoietic cells in patients after HSCT. Chimerism studies can help identify the source of hematopoiesis after engraftment, identify graft rejection in patients with poor hematologic recovery, and assist in the diagnosis and prediction of relapse and clinical graft-vs-host disease (GVHD). The most commonly applied method is PCR amplification of short tandem repeats (STR), a type of genetic polymorphism. The interpretation of chimerism results depends on the context in which the testing was done, i.e., the type of transplant, conditioning intensity, timing of testing post-transplant and results of concurrent blood counts and bone marrow morphology. While the methodology is technically straightforward, the implementation, analysis, and interpretation of results can be complicated. The clinical importance of chimerism results warrants the effort required by the laboratory for validation and performance of the test. In this chapter we review the clinical utility of chimerism analysis and laboratory aspects of testing, including methods, reagents, approaches to pre- and post-transplant testing, analysis and interpretation, pitfalls and limitations, reporting, and data management.
7 schema:editor N087ac3fb321c47908c565b5d017167e1
8 schema:genre chapter
9 schema:inLanguage en
10 schema:isAccessibleForFree false
11 schema:isPartOf N1852c56d797a41d49c0718fe3a30ee50
12 schema:keywords PCR amplification
13 allogeneic hematopoietic stem cell transplantation
14 amplification
15 analysis
16 aspects
17 blood count
18 bone marrow morphology
19 cell transplantation
20 cells
21 chapter
22 chimerism analysis
23 chimerism levels
24 chimerism results
25 chimerism studies
26 chimerism testing
27 clinical graft
28 clinical importance
29 clinical utility
30 concurrent blood counts
31 conditioning intensity
32 context
33 counts
34 data management
35 diagnosis
36 disease
37 donors
38 efforts
39 engraftment
40 genetic polymorphisms
41 graft
42 graft rejection
43 hematologic recovery
44 hematopoiesis
45 hematopoietic cells
46 hematopoietic stem cell transplantation
47 host disease
48 implementation
49 importance
50 intensity
51 interpretation
52 interpretation of results
53 laboratory
54 laboratory aspects
55 levels
56 limitations
57 management
58 marrow morphology
59 method
60 methodology
61 molecular analysis
62 morphology
63 nonmalignant diseases
64 options
65 patients
66 performance
67 pitfalls
68 polymorphism
69 poor hematologic recovery
70 post-transplant testing
71 prediction
72 prediction of relapse
73 proportion
74 proportion of donors
75 reagents
76 recovery
77 rejection
78 relapse
79 repeats
80 reporting
81 results
82 short tandem repeats
83 source
84 source of hematopoiesis
85 stem cell transplantation
86 study
87 tandem repeats
88 test
89 testing
90 timing
91 transplant
92 transplantation
93 treatment options
94 type of transplant
95 types
96 utility
97 validation
98 variety
99 years
100 schema:name Chimerism Testing in Allogeneic Hematopoietic Stem Cell Transplantation
101 schema:pagination 823-848
102 schema:productId N28dfe288671d41a8957d9b132e99d325
103 Ncedb177caeaa49e1965b0555d69ae820
104 schema:publisher N475f282dbc47477881f4d5b35187ff2b
105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019835104
106 https://doi.org/10.1007/978-3-319-19674-9_56
107 schema:sdDatePublished 2021-11-01T18:54
108 schema:sdLicense https://scigraph.springernature.com/explorer/license/
109 schema:sdPublisher N854515a2bce8494ba496db07eafdb852
110 schema:url https://doi.org/10.1007/978-3-319-19674-9_56
111 sgo:license sg:explorer/license/
112 sgo:sdDataset chapters
113 rdf:type schema:Chapter
114 N087ac3fb321c47908c565b5d017167e1 rdf:first Nbd0d23c05a3245f88762b778b85b537c
115 rdf:rest rdf:nil
116 N1852c56d797a41d49c0718fe3a30ee50 schema:isbn 978-3-319-19673-2
117 978-3-319-19674-9
118 schema:name Molecular Pathology in Clinical Practice
119 rdf:type schema:Book
120 N28dfe288671d41a8957d9b132e99d325 schema:name doi
121 schema:value 10.1007/978-3-319-19674-9_56
122 rdf:type schema:PropertyValue
123 N475f282dbc47477881f4d5b35187ff2b schema:name Springer Nature
124 rdf:type schema:Organisation
125 N62aef916d9dc4634a24cbd60e54bdaee rdf:first sg:person.014435572617.39
126 rdf:rest Nf34986617eb743219b018c93c17f5e91
127 N854515a2bce8494ba496db07eafdb852 schema:name Springer Nature - SN SciGraph project
128 rdf:type schema:Organization
129 Nbd0d23c05a3245f88762b778b85b537c schema:familyName Leonard
130 schema:givenName Debra G.B.
131 rdf:type schema:Person
132 Ncedb177caeaa49e1965b0555d69ae820 schema:name dimensions_id
133 schema:value pub.1019835104
134 rdf:type schema:PropertyValue
135 Nf34986617eb743219b018c93c17f5e91 rdf:first sg:person.01267452463.62
136 rdf:rest rdf:nil
137 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
138 schema:name Medical and Health Sciences
139 rdf:type schema:DefinedTerm
140 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
141 schema:name Clinical Sciences
142 rdf:type schema:DefinedTerm
143 sg:person.01267452463.62 schema:affiliation grid-institutes:grid.25879.31
144 schema:familyName Reshef
145 schema:givenName Ran
146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01267452463.62
147 rdf:type schema:Person
148 sg:person.014435572617.39 schema:affiliation grid-institutes:grid.25879.31
149 schema:familyName Van Deerlin
150 schema:givenName Vivianna M.
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014435572617.39
152 rdf:type schema:Person
153 grid-institutes:grid.25879.31 schema:alternateName Department of Medicine, Hematology & Oncology Division, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA
154 Pathology and Laboratory Medicine, Molecular Pathology Laboratory, Perelman School of Medicine at the University of Pennsylvania, 3400 Spruce St., 7.103 Founders Pavilion, 19104, Philadelphia, PA, USA
155 schema:name Department of Medicine, Hematology & Oncology Division, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA
156 Pathology and Laboratory Medicine, Molecular Pathology Laboratory, Perelman School of Medicine at the University of Pennsylvania, 3400 Spruce St., 7.103 Founders Pavilion, 19104, Philadelphia, PA, USA
157 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...