Bone Marrow Work-up: Report of a Pilot Study View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2015

AUTHORS

Ioannis Anagnostopoulos , Dido Lenze , Michael Hummel , Manfred Dietel , Korinna Jöhrens

ABSTRACT

In this pilot study we changed several pre-analytical variables of bone marrow trephine biopsy processing with the task to achieve not only a preservation of morphology and antigens but also of nucleic acids. The changes involved employment of a newly established decalcification solution in conjunction with a short fixation time (2 h after receiving the specimens) and performance of decalcification at 37 °C. The comparison of the obtained results from three specimens with those of our routinely established protocol unequivocally revealed that the novel decalcification solution results in a superior preservation of nucleic acids, with only slight differences in preservation of morphology and cellular antigens. These encouraging results imply that this novel decalcification solution will allow a widely accepted standardization of bone marrow trephine biopsy processing. More... »

PAGES

95-105

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-319-13957-9_10

DOI

http://dx.doi.org/10.1007/978-3-319-13957-9_10

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049193738

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25636433


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