Ontology type: schema:Chapter
2014-10-01
AUTHORS ABSTRACTThe thrombotic cellular mechanisms associated with cardiovascular events remain unclear, largely because of an inability to visualize thrombus formation in vivo. In this chapter, bio-imaging approach is introduced, mainly using in vivo imaging technique based on single-photon and multiphoton microscopy. Recent progress in optics enabled us to identify single-platelet behavior in the developing thrombus in living animals. In the physiological and pathological conditions, complex cellular interplay takes place, and bio-imaging can directly elucidate cross talks among multiple cell types. We visualized that rapidly developing thrombi composed of discoid platelets were triggered by reactive oxygen species photochemically induced by laser irradiation, and developed thrombi occluded blood vessels. Inflammatory cytokine and adhesion molecules dynamically control these processes. Intravital imaging can be a powerful tool to analyze thrombus formation and evaluate the therapeutic strategies against thrombotic diseases. More... »
PAGES171-184
Cardiovascular Imaging
ISBN
978-3-319-09267-6
978-3-319-09268-3
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