Electrochemical Immunoassay for Mucin 1 Detection as a Diagnostic Tool in Ovarian Cancer View Full Text


Ontology type: schema:Chapter      Open Access: True


Chapter Info

DATE

2014

AUTHORS

Andrea Ravalli , Giovanna Marrazza , Anca Florea , Cecilia Cristea , Robert Sandulescu

ABSTRACT

The immunoassay developed for Mucin 1 (MUC1) detection is based on a sandwich format in which a primary antibody immobilized on the surface of magnetic beads specifically binds the MUC1 protein. The sandwich immunoassay is performed by adding secondary anti-MUC1 and an alkaline-phosphatase-labeled third antibody. After, the modified magnetic beads are captured by a magnet on the surface of a graphite working electrode, and the electrochemical detection is thus achieved through the addition of 1-naphthyl phosphate. 1-naphthol, produced during the enzymatic reaction, is detected using differential pulse voltammetry (DPV). The performance of the assay in terms of sensitivity, reproducibility, and selectivity has been studied. MUC1 detection is performed in buffered solutions and serum sample. More... »

PAGES

165-168

References to SciGraph publications

  • 2004-12. MUC1: A multifunctional cell surface component of reproductive tissue epithelia in REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
  • Book

    TITLE

    Sensors and Microsystems

    ISBN

    978-3-319-00683-3
    978-3-319-00684-0

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-3-319-00684-0_31

    DOI

    http://dx.doi.org/10.1007/978-3-319-00684-0_31

    DIMENSIONS

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