Determination of 8-oxo-purines in DNA by HPLC using amperometric detection View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1995

AUTHORS

T. Douki , M. Berger , S. Raoul , J.-L. Ravanat , J. Cadet

ABSTRACT

High-performance liquid chromatography coupled with electrochemical detection is a sensitive assay for 8-oxo-purines derivatives. This technique was applied to the measurement of the rate of formation of 8- oxo-dGuo in photooxidized isolated DNA following either enzymatic digestion or acidic hydrolysis. The assay was also used to assess the excision of 8-oxo-Gua from DNA upon incubation with the Fapy DNA glycosylase protein. It also allowed the determination of the respective yield of 8-oxo-dGuo and 8-oxo-dAdo upon γ-radiolysis of DNA in aqueous aerated solution. More... »

PAGES

213-224

Book

TITLE

Analysis of Free Radicals in Biological Systems

ISBN

978-3-0348-9895-9
978-3-0348-9074-8

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-0348-9074-8_15

DOI

http://dx.doi.org/10.1007/978-3-0348-9074-8_15

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026084572


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