Apoptosis of human kidney 293 cells is promoted by polymerized Cd-metallothionein View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

1999

AUTHORS

Tetsuo Hamada , Akihide Tanimoto , Takakazu Sasaguri , Nobuyuki Arima , Shohei Shimajiri , Ryuji Nakano , Yasuyuki Sasaguri

ABSTRACT

Exposure to 12.5 to 37.5 µM CdCl2 induced apoptosis in transformed human kidney cells (293 cells) as confirmed by characteristic ultrastructural features, a ladder on gel electrophoresis of extracted DNA, and fragmentation of nucleosomes as detected by enzyme linked immunosorbent assay (ELISA). Higher concentrations of Cd were less effective in inducing apoptosis. Furthermore, addition of the protein extract from the serum-free medium used for Cd-exposure promoted apoptosis exhibiting the same features as that after Cd-exposure. The apotosis induced by the protein was dose-dependent. The molecular weight of the protein (Cd-protein) was shown to be 40 kDa by gel filtration. Two-dimensional electrophoresis revealed the Cd-protein as a single spot with a molecular weight of 6 kDa and pI of 4.5. Competitive ELISA showed that the Cd-protein reacted with anti-metallothionein antibody. The present findings suggest that apoptosis is induced not only by Cd itself but also by polymerized metallothionein (MT) molecules released from cells into the medium. More... »

PAGES

489-495

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-0348-8847-9_72

DOI

http://dx.doi.org/10.1007/978-3-0348-8847-9_72

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052875938


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