Anticoronavirus Activity of Water-Soluble Pristine C60 Fullerenes: In Vitro and In SilicoScreenings View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2021

AUTHORS

Vasyl Hurmach , Maxim Platonov , Svitlana Prylutska , Zinaida Klestova , Vsevolod Cherepanov , Yuriy Prylutskyy , Uwe Ritter

ABSTRACT

Introduction: The emergence of a new member of the Coronaviridae family, which caused the 2020 pandemic, requires detailed research on the evolution of coronaviruses, their structure and properties, and interaction with cells. Modern nanobiotechnologies can address the many clinical challenges posed by the COVID-19 pandemic. In particular, they offer new therapeutic approaches using biocompatible nanostructures with “specific” antiviral activity. Therefore, the nanosized spherical-like molecule (0.72 nm in diameter) composed of 60 carbon atoms, C60 fullerene, is of interest in terms of fighting coronaviruses due to its high biological activity. In here, we aim to evaluate the effectiveness of anticoronavirus action of water-soluble pristine C60 fullerene in the model and in vitro systems. As a model, apathogenic for human coronavirus, we used transmissible gastroenteritis virus of swine (TGEV), which we adapted to the BHK-21 cell culture (kidney cells of a newborn Syrian hamster).Methods: The shape and size of the particles present in C60 fullerene aqueous colloidal solution (C60FAS) of given concentration, as well as C60FAS stability (value of zeta potential) were studied using microscopic (STM, scanning tunneling microscopy, and AFM, atomic force microscopy) and spectroscopic (DLS, dynamic light scattering) methods. The cytopathic effect of TGEV was determined with the help of a Leica DM 750 microscope and the degree of monolayer changes in cells was assessed. The microscopy of the viral suspension was performed using a high resolution transmission electron microscope (HRTEM; JEM-1230, Japan). Finally, the search for and design of optimal possible complexes between C60 fullerene and target proteins in the structure of SARS-CoV-2 coronavirus, evaluation of their stability in the simulated cellular environment were performed using molecular dynamics and docking methods.Results: It was found that the maximum allowable cytotoxic concentration of C60 fullerene is 37.5 ± 3.0 μg/ml. The investigated C60FAS reduces the titer of coronavirus infectious activity by the value of 2.00 ± 0.08 TCID50/ml. It was shown that C60 fullerene interacts directly with SARS-CoV-2 proteins, such as RdRp (RNA-dependent RNA polymerase) and 3CLpro (3-chymotrypsin-like protease), which is critical for the life cycle of the coronavirus and, thus, inhibits its functional activity. In both cases, C60 fullerene fills the binding pocket and gets stuck there through stacking and steric interactions.Conclusion: Pioneer in vitro study to identify the anticoronavirus activity of water-soluble pristine C60 fullerenes indicates that they are highly promising for further preclinical studies, since a significant inhibition of the infectious activity of swine coronavirus of transmissible gastroenteritis in BHK-21 cell culture was found. According to molecular modeling results, it was shown that C60 fullerene can create the stable complexes with 3CLpro and RdRp proteins of SARS-CoV-2 coronavirus and, thus, suppress its functional activity. More... »

PAGES

159-172

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-030-85109-5_10

DOI

http://dx.doi.org/10.1007/978-3-030-85109-5_10

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1145335533

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/35132600


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "COVID-19", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fullerenes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pandemics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "SARS-CoV-2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Swine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Water", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Taras Shevchenko National University of Kyiv, Kyiv, Ukraine", 
          "id": "http://www.grid.ac/institutes/grid.34555.32", 
          "name": [
            "Taras Shevchenko National University of Kyiv, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hurmach", 
        "givenName": "Vasyl", 
        "id": "sg:person.0633354003.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633354003.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine", 
          "id": "http://www.grid.ac/institutes/grid.418824.3", 
          "name": [
            "Institute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Platonov", 
        "givenName": "Maxim", 
        "id": "sg:person.01316152143.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316152143.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National University of Life and Environmental Science of Ukraine, Kyiv, Ukraine", 
          "id": "http://www.grid.ac/institutes/grid.37677.32", 
          "name": [
            "Taras Shevchenko National University of Kyiv, Kyiv, Ukraine", 
            "National University of Life and Environmental Science of Ukraine, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Prylutska", 
        "givenName": "Svitlana", 
        "id": "sg:person.01265014537.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265014537.58"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "State Scientific-Control Institute of Biotechnology and Strains of Microorganisms, Kyiv, Ukraine", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "State Scientific-Control Institute of Biotechnology and Strains of Microorganisms, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Klestova", 
        "givenName": "Zinaida", 
        "id": "sg:person.011447622733.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011447622733.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Physics of NASU, Kyiv, Ukraine", 
          "id": "http://www.grid.ac/institutes/grid.425082.9", 
          "name": [
            "Institute of Physics of NASU, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cherepanov", 
        "givenName": "Vsevolod", 
        "id": "sg:person.0772065437.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772065437.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Taras Shevchenko National University of Kyiv, Kyiv, Ukraine", 
          "id": "http://www.grid.ac/institutes/grid.34555.32", 
          "name": [
            "Taras Shevchenko National University of Kyiv, Kyiv, Ukraine"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Prylutskyy", 
        "givenName": "Yuriy", 
        "id": "sg:person.0761452727.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0761452727.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Technical University of Ilmenau, Ilmenau, Germany", 
          "id": "http://www.grid.ac/institutes/grid.6553.5", 
          "name": [
            "Technical University of Ilmenau, Ilmenau, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ritter", 
        "givenName": "Uwe", 
        "id": "sg:person.0664526675.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664526675.56"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2021", 
    "datePublishedReg": "2021-01-01", 
    "description": "Introduction: The emergence of a new member of the Coronaviridae family, which caused the 2020 pandemic, requires detailed research on the evolution of coronaviruses, their structure and properties, and interaction with cells. Modern nanobiotechnologies can address the many clinical challenges posed by the COVID-19 pandemic. In particular, they offer new therapeutic approaches using biocompatible nanostructures with \u201cspecific\u201d antiviral activity. Therefore, the nanosized spherical-like molecule (0.72 nm in diameter) composed of 60 carbon atoms, C60 fullerene, is of interest in terms of fighting coronaviruses due to its high biological activity. In here, we aim to evaluate the effectiveness of anticoronavirus action of water-soluble pristine C60 fullerene in the model and in vitro systems. As a model, apathogenic for human coronavirus, we used transmissible gastroenteritis virus of swine (TGEV), which we adapted to the BHK-21 cell culture (kidney cells of a newborn Syrian hamster).Methods: The shape and size of the particles present in C60 fullerene aqueous colloidal solution (C60FAS) of given concentration, as well as C60FAS stability (value of zeta potential) were studied using microscopic (STM, scanning tunneling microscopy, and AFM, atomic force microscopy) and spectroscopic (DLS, dynamic light scattering) methods. The cytopathic effect of TGEV was determined with the help of a Leica DM 750 microscope and the degree of monolayer changes in cells was assessed. The microscopy of the viral suspension was performed using a high resolution transmission electron microscope (HRTEM; JEM-1230, Japan). Finally, the search for and design of optimal possible complexes between C60 fullerene and target proteins in the structure of SARS-CoV-2 coronavirus, evaluation of their stability in the simulated cellular environment were performed using molecular dynamics and docking methods.Results: It was found that the maximum allowable cytotoxic concentration of C60 fullerene is 37.5\u00a0\u00b1\u00a03.0\u00a0\u03bcg/ml. The investigated C60FAS reduces the titer of coronavirus infectious activity by the value of 2.00\u00a0\u00b1\u00a00.08\u00a0TCID50/ml. It was shown that C60 fullerene interacts directly with SARS-CoV-2 proteins, such as RdRp (RNA-dependent RNA polymerase) and 3CLpro (3-chymotrypsin-like protease), which is critical for the life cycle of the coronavirus and, thus, inhibits its functional activity. In both cases, C60 fullerene fills the binding pocket and gets stuck there through stacking and steric interactions.Conclusion: Pioneer in vitro study to identify the anticoronavirus activity of water-soluble pristine C60 fullerenes indicates that they are highly promising for further preclinical studies, since a significant inhibition of the infectious activity of swine coronavirus of transmissible gastroenteritis in BHK-21 cell culture was found. According to molecular modeling results, it was shown that C60 fullerene can create the stable complexes with 3CLpro and RdRp proteins of SARS-CoV-2 coronavirus and, thus, suppress its functional activity.", 
    "editor": [
      {
        "familyName": "Asea", 
        "givenName": "Alexzander A. A.", 
        "type": "Person"
      }, 
      {
        "familyName": "Kaur", 
        "givenName": "Punit", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-3-030-85109-5_10", 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-3-030-85108-8", 
        "978-3-030-85109-5"
      ], 
      "name": "Coronavirus Therapeutics \u2013 Volume I", 
      "type": "Book"
    }, 
    "keywords": [
      "high-resolution transmission electron microscope", 
      "resolution transmission electron microscope", 
      "aqueous colloidal solutions", 
      "transmission electron microscope", 
      "biocompatible nanostructures", 
      "modern nanobiotechnology", 
      "colloidal solution", 
      "C60 fullerene", 
      "pristine C60 fullerene", 
      "electron microscope", 
      "fullerenes", 
      "microscope", 
      "TCID50/", 
      "nanobiotechnology", 
      "monolayer changes", 
      "nanostructures", 
      "cell cultures", 
      "cellular environment", 
      "high biological activity", 
      "molecular dynamics", 
      "target proteins", 
      "infectious activity", 
      "swine coronaviruses", 
      "carbon atoms", 
      "microscopy", 
      "viral suspension", 
      "transmissible gastroenteritis virus", 
      "stability", 
      "spectroscopic methods", 
      "molecular modeling results", 
      "gastroenteritis virus", 
      "atoms", 
      "structure", 
      "particles", 
      "biological activity", 
      "possible complexes", 
      "size", 
      "properties", 
      "suspension", 
      "C60", 
      "method", 
      "cells", 
      "concentration", 
      "design", 
      "interaction", 
      "preclinical studies", 
      "microscopic", 
      "challenges", 
      "steric interactions", 
      "further preclinical studies", 
      "solution", 
      "transmissible gastroenteritis", 
      "activity", 
      "modeling results", 
      "dynamics", 
      "titers", 
      "shape", 
      "protein", 
      "TGEV", 
      "functional activity", 
      "molecules", 
      "system", 
      "cytopathic effect", 
      "new member", 
      "environment", 
      "evolution", 
      "vitro", 
      "interest", 
      "stable complexes", 
      "RdRp protein", 
      "docking methods", 
      "therapeutic approaches", 
      "cytotoxic concentrations", 
      "cycle", 
      "help", 
      "BHK-21 cell cultures", 
      "approach", 
      "clinical challenge", 
      "new therapeutic approaches", 
      "virus", 
      "model", 
      "culture", 
      "complexes", 
      "significant inhibition", 
      "antiviral activity", 
      "results", 
      "terms", 
      "detailed research", 
      "SARS-CoV-2 proteins", 
      "human coronaviruses", 
      "effect", 
      "values", 
      "research", 
      "search", 
      "degree", 
      "evaluation", 
      "study", 
      "RdRp", 
      "pocket", 
      "life cycle", 
      "emergence", 
      "effectiveness", 
      "SARS-CoV-2 coronavirus", 
      "coronavirus", 
      "cases", 
      "swine", 
      "changes", 
      "pioneers", 
      "inhibition", 
      "gastroenteritis", 
      "family", 
      "action", 
      "Coronaviridae family", 
      "anticoronavirus activity", 
      "pandemic", 
      "members", 
      "COVID-19 pandemic", 
      "evolution of coronaviruses", 
      "C60FAS"
    ], 
    "name": "Anticoronavirus Activity of Water-Soluble Pristine C60 Fullerenes: In Vitro and In SilicoScreenings", 
    "pagination": "159-172", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1145335533"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-3-030-85109-5_10"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "35132600"
        ]
      }
    ], 
    "publisher": {
      "name": "Springer Nature", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-3-030-85109-5_10", 
      "https://app.dimensions.ai/details/publication/pub.1145335533"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2022-12-01T06:50", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/chapter/chapter_283.jsonl", 
    "type": "Chapter", 
    "url": "https://doi.org/10.1007/978-3-030-85109-5_10"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-3-030-85109-5_10'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-3-030-85109-5_10'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-3-030-85109-5_10'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-3-030-85109-5_10'


 

This table displays all metadata directly associated to this object as RDF triples.

273 TRIPLES      22 PREDICATES      152 URIs      146 LITERALS      16 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-3-030-85109-5_10 schema:about N27d56380f5d243558067b1aa131fe1e1
2 N37de5b4f29db435c92799a22ab70fbb2
3 N4157b9da71304eeb87ab5f065b373bc2
4 N8979f96506fc4a15b3550165e0facaf6
5 N8c2ab3fe62044573955aa461ae3aad3c
6 Na6f09a42d88c4f3aa3c01d9f6dbb1061
7 Ncbeddc3f6d91488ca6ba250467e6eab8
8 Nd87a80fc03de4d658c5b06b9c0191e01
9 anzsrc-for:11
10 schema:author N53f307269902441387a988f6ed0b7822
11 schema:datePublished 2021
12 schema:datePublishedReg 2021-01-01
13 schema:description Introduction: The emergence of a new member of the Coronaviridae family, which caused the 2020 pandemic, requires detailed research on the evolution of coronaviruses, their structure and properties, and interaction with cells. Modern nanobiotechnologies can address the many clinical challenges posed by the COVID-19 pandemic. In particular, they offer new therapeutic approaches using biocompatible nanostructures with “specific” antiviral activity. Therefore, the nanosized spherical-like molecule (0.72 nm in diameter) composed of 60 carbon atoms, C60 fullerene, is of interest in terms of fighting coronaviruses due to its high biological activity. In here, we aim to evaluate the effectiveness of anticoronavirus action of water-soluble pristine C60 fullerene in the model and in vitro systems. As a model, apathogenic for human coronavirus, we used transmissible gastroenteritis virus of swine (TGEV), which we adapted to the BHK-21 cell culture (kidney cells of a newborn Syrian hamster).Methods: The shape and size of the particles present in C60 fullerene aqueous colloidal solution (C60FAS) of given concentration, as well as C60FAS stability (value of zeta potential) were studied using microscopic (STM, scanning tunneling microscopy, and AFM, atomic force microscopy) and spectroscopic (DLS, dynamic light scattering) methods. The cytopathic effect of TGEV was determined with the help of a Leica DM 750 microscope and the degree of monolayer changes in cells was assessed. The microscopy of the viral suspension was performed using a high resolution transmission electron microscope (HRTEM; JEM-1230, Japan). Finally, the search for and design of optimal possible complexes between C60 fullerene and target proteins in the structure of SARS-CoV-2 coronavirus, evaluation of their stability in the simulated cellular environment were performed using molecular dynamics and docking methods.Results: It was found that the maximum allowable cytotoxic concentration of C60 fullerene is 37.5 ± 3.0 μg/ml. The investigated C60FAS reduces the titer of coronavirus infectious activity by the value of 2.00 ± 0.08 TCID50/ml. It was shown that C60 fullerene interacts directly with SARS-CoV-2 proteins, such as RdRp (RNA-dependent RNA polymerase) and 3CLpro (3-chymotrypsin-like protease), which is critical for the life cycle of the coronavirus and, thus, inhibits its functional activity. In both cases, C60 fullerene fills the binding pocket and gets stuck there through stacking and steric interactions.Conclusion: Pioneer in vitro study to identify the anticoronavirus activity of water-soluble pristine C60 fullerenes indicates that they are highly promising for further preclinical studies, since a significant inhibition of the infectious activity of swine coronavirus of transmissible gastroenteritis in BHK-21 cell culture was found. According to molecular modeling results, it was shown that C60 fullerene can create the stable complexes with 3CLpro and RdRp proteins of SARS-CoV-2 coronavirus and, thus, suppress its functional activity.
14 schema:editor N364c534038d047eb9e7a1e07dfc68184
15 schema:genre chapter
16 schema:isAccessibleForFree false
17 schema:isPartOf N76091709b85142ce85118746c2c30d96
18 schema:keywords BHK-21 cell cultures
19 C60
20 C60 fullerene
21 C60FAS
22 COVID-19 pandemic
23 Coronaviridae family
24 RdRp
25 RdRp protein
26 SARS-CoV-2 coronavirus
27 SARS-CoV-2 proteins
28 TCID50/
29 TGEV
30 action
31 activity
32 anticoronavirus activity
33 antiviral activity
34 approach
35 aqueous colloidal solutions
36 atoms
37 biocompatible nanostructures
38 biological activity
39 carbon atoms
40 cases
41 cell cultures
42 cells
43 cellular environment
44 challenges
45 changes
46 clinical challenge
47 colloidal solution
48 complexes
49 concentration
50 coronavirus
51 culture
52 cycle
53 cytopathic effect
54 cytotoxic concentrations
55 degree
56 design
57 detailed research
58 docking methods
59 dynamics
60 effect
61 effectiveness
62 electron microscope
63 emergence
64 environment
65 evaluation
66 evolution
67 evolution of coronaviruses
68 family
69 fullerenes
70 functional activity
71 further preclinical studies
72 gastroenteritis
73 gastroenteritis virus
74 help
75 high biological activity
76 high-resolution transmission electron microscope
77 human coronaviruses
78 infectious activity
79 inhibition
80 interaction
81 interest
82 life cycle
83 members
84 method
85 microscope
86 microscopic
87 microscopy
88 model
89 modeling results
90 modern nanobiotechnology
91 molecular dynamics
92 molecular modeling results
93 molecules
94 monolayer changes
95 nanobiotechnology
96 nanostructures
97 new member
98 new therapeutic approaches
99 pandemic
100 particles
101 pioneers
102 pocket
103 possible complexes
104 preclinical studies
105 pristine C60 fullerene
106 properties
107 protein
108 research
109 resolution transmission electron microscope
110 results
111 search
112 shape
113 significant inhibition
114 size
115 solution
116 spectroscopic methods
117 stability
118 stable complexes
119 steric interactions
120 structure
121 study
122 suspension
123 swine
124 swine coronaviruses
125 system
126 target proteins
127 terms
128 therapeutic approaches
129 titers
130 transmissible gastroenteritis
131 transmissible gastroenteritis virus
132 transmission electron microscope
133 values
134 viral suspension
135 virus
136 vitro
137 schema:name Anticoronavirus Activity of Water-Soluble Pristine C60 Fullerenes: In Vitro and In SilicoScreenings
138 schema:pagination 159-172
139 schema:productId N1a72910af5f74a5e8a0f10f192f771b2
140 N31d4dfa6ab9f44ae8b318163214b96a8
141 Nc1c1e428463e49e6ad7f3c7c22e1c4b0
142 schema:publisher N4499d905f48948ed977059c7dbcb251a
143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1145335533
144 https://doi.org/10.1007/978-3-030-85109-5_10
145 schema:sdDatePublished 2022-12-01T06:50
146 schema:sdLicense https://scigraph.springernature.com/explorer/license/
147 schema:sdPublisher N00807ec8206346519861084aac05ab87
148 schema:url https://doi.org/10.1007/978-3-030-85109-5_10
149 sgo:license sg:explorer/license/
150 sgo:sdDataset chapters
151 rdf:type schema:Chapter
152 N00807ec8206346519861084aac05ab87 schema:name Springer Nature - SN SciGraph project
153 rdf:type schema:Organization
154 N05b93a71609a49d196dd6e5f6dfc21c9 rdf:first sg:person.011447622733.51
155 rdf:rest N4a836368d0214c33a81c20643e97b1e0
156 N188410db4a22436c878d34a137fe013c schema:familyName Kaur
157 schema:givenName Punit
158 rdf:type schema:Person
159 N1a72910af5f74a5e8a0f10f192f771b2 schema:name dimensions_id
160 schema:value pub.1145335533
161 rdf:type schema:PropertyValue
162 N27d56380f5d243558067b1aa131fe1e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Pandemics
164 rdf:type schema:DefinedTerm
165 N31d4dfa6ab9f44ae8b318163214b96a8 schema:name doi
166 schema:value 10.1007/978-3-030-85109-5_10
167 rdf:type schema:PropertyValue
168 N364c534038d047eb9e7a1e07dfc68184 rdf:first N9816c85f2bdc44d4aa74c02ff5ee1b3a
169 rdf:rest Nc3d0eecc5d8549608844a22a290e879e
170 N37de5b4f29db435c92799a22ab70fbb2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
171 schema:name Water
172 rdf:type schema:DefinedTerm
173 N3d7109351c6345f2ad686ec17303d9a3 rdf:first sg:person.01265014537.58
174 rdf:rest N05b93a71609a49d196dd6e5f6dfc21c9
175 N4157b9da71304eeb87ab5f065b373bc2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name SARS-CoV-2
177 rdf:type schema:DefinedTerm
178 N4499d905f48948ed977059c7dbcb251a schema:name Springer Nature
179 rdf:type schema:Organisation
180 N4a836368d0214c33a81c20643e97b1e0 rdf:first sg:person.0772065437.14
181 rdf:rest N598c10b263154b0b937a3d004755b1ba
182 N53f307269902441387a988f6ed0b7822 rdf:first sg:person.0633354003.03
183 rdf:rest Nd682b704f27d411aaa96cb85e9360924
184 N598c10b263154b0b937a3d004755b1ba rdf:first sg:person.0761452727.99
185 rdf:rest Ne6d5dc7975674264a9e5027d3ebc6293
186 N76091709b85142ce85118746c2c30d96 schema:isbn 978-3-030-85108-8
187 978-3-030-85109-5
188 schema:name Coronavirus Therapeutics – Volume I
189 rdf:type schema:Book
190 N8979f96506fc4a15b3550165e0facaf6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
191 schema:name COVID-19
192 rdf:type schema:DefinedTerm
193 N8c2ab3fe62044573955aa461ae3aad3c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
194 schema:name Animals
195 rdf:type schema:DefinedTerm
196 N9816c85f2bdc44d4aa74c02ff5ee1b3a schema:familyName Asea
197 schema:givenName Alexzander A. A.
198 rdf:type schema:Person
199 Na6f09a42d88c4f3aa3c01d9f6dbb1061 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
200 schema:name Humans
201 rdf:type schema:DefinedTerm
202 Nc1c1e428463e49e6ad7f3c7c22e1c4b0 schema:name pubmed_id
203 schema:value 35132600
204 rdf:type schema:PropertyValue
205 Nc3d0eecc5d8549608844a22a290e879e rdf:first N188410db4a22436c878d34a137fe013c
206 rdf:rest rdf:nil
207 Ncbeddc3f6d91488ca6ba250467e6eab8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
208 schema:name Swine
209 rdf:type schema:DefinedTerm
210 Nd682b704f27d411aaa96cb85e9360924 rdf:first sg:person.01316152143.09
211 rdf:rest N3d7109351c6345f2ad686ec17303d9a3
212 Nd87a80fc03de4d658c5b06b9c0191e01 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
213 schema:name Fullerenes
214 rdf:type schema:DefinedTerm
215 Ne6d5dc7975674264a9e5027d3ebc6293 rdf:first sg:person.0664526675.56
216 rdf:rest rdf:nil
217 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
218 schema:name Medical and Health Sciences
219 rdf:type schema:DefinedTerm
220 sg:person.011447622733.51 schema:affiliation grid-institutes:None
221 schema:familyName Klestova
222 schema:givenName Zinaida
223 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011447622733.51
224 rdf:type schema:Person
225 sg:person.01265014537.58 schema:affiliation grid-institutes:grid.37677.32
226 schema:familyName Prylutska
227 schema:givenName Svitlana
228 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265014537.58
229 rdf:type schema:Person
230 sg:person.01316152143.09 schema:affiliation grid-institutes:grid.418824.3
231 schema:familyName Platonov
232 schema:givenName Maxim
233 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316152143.09
234 rdf:type schema:Person
235 sg:person.0633354003.03 schema:affiliation grid-institutes:grid.34555.32
236 schema:familyName Hurmach
237 schema:givenName Vasyl
238 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633354003.03
239 rdf:type schema:Person
240 sg:person.0664526675.56 schema:affiliation grid-institutes:grid.6553.5
241 schema:familyName Ritter
242 schema:givenName Uwe
243 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664526675.56
244 rdf:type schema:Person
245 sg:person.0761452727.99 schema:affiliation grid-institutes:grid.34555.32
246 schema:familyName Prylutskyy
247 schema:givenName Yuriy
248 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0761452727.99
249 rdf:type schema:Person
250 sg:person.0772065437.14 schema:affiliation grid-institutes:grid.425082.9
251 schema:familyName Cherepanov
252 schema:givenName Vsevolod
253 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0772065437.14
254 rdf:type schema:Person
255 grid-institutes:None schema:alternateName State Scientific-Control Institute of Biotechnology and Strains of Microorganisms, Kyiv, Ukraine
256 schema:name State Scientific-Control Institute of Biotechnology and Strains of Microorganisms, Kyiv, Ukraine
257 rdf:type schema:Organization
258 grid-institutes:grid.34555.32 schema:alternateName Taras Shevchenko National University of Kyiv, Kyiv, Ukraine
259 schema:name Taras Shevchenko National University of Kyiv, Kyiv, Ukraine
260 rdf:type schema:Organization
261 grid-institutes:grid.37677.32 schema:alternateName National University of Life and Environmental Science of Ukraine, Kyiv, Ukraine
262 schema:name National University of Life and Environmental Science of Ukraine, Kyiv, Ukraine
263 Taras Shevchenko National University of Kyiv, Kyiv, Ukraine
264 rdf:type schema:Organization
265 grid-institutes:grid.418824.3 schema:alternateName Institute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine
266 schema:name Institute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine
267 rdf:type schema:Organization
268 grid-institutes:grid.425082.9 schema:alternateName Institute of Physics of NASU, Kyiv, Ukraine
269 schema:name Institute of Physics of NASU, Kyiv, Ukraine
270 rdf:type schema:Organization
271 grid-institutes:grid.6553.5 schema:alternateName Technical University of Ilmenau, Ilmenau, Germany
272 schema:name Technical University of Ilmenau, Ilmenau, Germany
273 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...