Gene Expression of Prox-1 and Hif-1a in Primo Vessels Inside Lymph Vessels of the Rabbit View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2021-05-09

AUTHORS

Jun-Young Shin , Jong-Gu Choi , Sungchul Shin , Sujung Yeo , Sang-Suk Lee

ABSTRACT

The gene expression of Prox-1 and Hif-1a for the isolated primo vessels (PVs) and composite lymphatic vessels (LVs) containing PVs (LVs + PVs) was investigated by RNA-sequencing (Seq) and quantitative polymerase chain reaction (qRT-PCR) analysis. RNA-Seq on the passed 10 samples on RNA-QC for two experimental groups with PVs and PVs + LVs proceeded to the library construction stage automatically and analyzed differentially expressed genes (DEGs). From the real-time qRT-PCR analysis data, we found the marker genes of Prox-1 and Hif-1a were enriched and decreased in an isolated PVs compared to LVs, respectively. Based on mRNA transcriptional data, Prox-1 and Hif-1a were increased and decreased in PVs compared to LVs + PVs under lipopolysaccharide (LPS) treatment and relieved by acupuncture electric stimulation (AES), respectively. This finding indicates that high and low levels of Prox-1 and Hif-1a may be involved in the function of PVs and that pathophysiological and physiological condition could progress into inflamed lymphatic endothelial cells expanding the PV within the LV. More... »

PAGES

387-391

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-3-030-48238-1_61

DOI

http://dx.doi.org/10.1007/978-3-030-48238-1_61

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1137859963

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/33966247


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204 Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea
205 rdf:type schema:Organization
 




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