Experimental Methods for Protein Interaction Identification and Characterization View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2008

AUTHORS

Peter Uetz , Björn Titz , Gerard Cagney

ABSTRACT

There are dozens of methods for the detection of protein-protein interactions but they fall into a few broad categories. Fragment complementation assays such as the yeast two-hybrid (Y2H) system are based on split proteins that are functionally reconstituted by fusions of interacting proteins. Biophysical methods include structure determination and mass spectrometric (MS) identification of proteins in complexes. Biochemical methods include methods such as far western blotting and peptide arrays. Only the Y2H and protein complex purification combined with MS have been used on a larger scale. Due to the lack of data it is still difficult to compare these methods with respect to their efficiency and error rates. Current data does not favor any particular method and thus multiple experimental approaches are necessary to maximally cover the interactome of any target cell or organism. More... »

PAGES

1-32

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-84800-125-1_1

DOI

http://dx.doi.org/10.1007/978-1-84800-125-1_1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031140018


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