Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2014

AUTHORS

Silvia Andrea Camperi , María Camila Martínez-Ceron , Silvana Laura Giudicessi , Mariela Mirta Marani , Fernando Albericio , Osvaldo Cascone

ABSTRACT

We describe a method to develop affinity chromatography matrices with short peptide ligands for protein purification. The method entitles the following: (a) synthesis of a combinatorial library on the hydromethylbenzoyl (HMBA)-ChemMatrix resin by the divide-couple-recombine (DCR) method using the Fmoc chemistry, (b) library screening with the protein of interest labeled with a fluorescent dye or biotin, (c) identification of peptides contained on positive beads by tandem matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS/MS), (d) solid-phase peptide ligand synthesis and immobilization in chromatographic supports, and (e) evaluation of protein adsorption on peptide affinity matrices from the equilibrium isotherms and breakthrough curves. More... »

PAGES

277-302

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-62703-977-2_22

DOI

http://dx.doi.org/10.1007/978-1-62703-977-2_22

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009289200

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24648083


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0305", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Organic Chemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromatography, Affinity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Combinatorial Chemistry Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tandem Mass Spectrometry", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Buenos Aires", 
          "id": "https://www.grid.ac/institutes/grid.7345.5", 
          "name": [
            "Institute of Nanobiotechnology UBA-CONICET, Cathedra of Industrial Microbiology and Biotechnology, School of Pharmacy and Biochemistry, University of Buenos Aires, Jun\u00edn 956, C1113AAD, Buenos Aires, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Camperi", 
        "givenName": "Silvia Andrea", 
        "id": "sg:person.01177030467.82", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177030467.82"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Buenos Aires", 
          "id": "https://www.grid.ac/institutes/grid.7345.5", 
          "name": [
            "Facultad de Farmacia y Bioqu\u00edmica, Universidad de Buenos Aires, Buenos Aires, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mart\u00ednez-Ceron", 
        "givenName": "Mar\u00eda Camila", 
        "id": "sg:person.0646206653.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646206653.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Buenos Aires", 
          "id": "https://www.grid.ac/institutes/grid.7345.5", 
          "name": [
            "Facultad de Farmacia y Bioqu\u00edmica, Universidad de Buenos Aires, Buenos Aires, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Giudicessi", 
        "givenName": "Silvana Laura", 
        "id": "sg:person.01270404403.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270404403.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Centro Nacional Patag\u00f3nico-CONICET, Puerto Madryn, Chubut, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Marani", 
        "givenName": "Mariela Mirta", 
        "id": "sg:person.0767177521.82", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0767177521.82"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Barcelona", 
          "id": "https://www.grid.ac/institutes/grid.5841.8", 
          "name": [
            "Instituto de Investigaciones Biom\u00e9dicas, Parque Cient\u00edfico de Barcelona, Universidad de Barcelona, Barcelona, Spain"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Albericio", 
        "givenName": "Fernando", 
        "id": "sg:person.01001414756.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001414756.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Buenos Aires", 
          "id": "https://www.grid.ac/institutes/grid.7345.5", 
          "name": [
            "Facultad de Farmacia y Bioqu\u00edmica, Universidad de Buenos Aires, Buenos Aires, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cascone", 
        "givenName": "Osvaldo", 
        "id": "sg:person.01062160206.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01062160206.02"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/s0040-4039(00)88568-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000456847"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(97)89038-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002270197"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/0-306-47059-4_7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010827994", 
          "https://doi.org/10.1007/0-306-47059-4_7"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/b514349g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011648275"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/b514349g", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011648275"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0040-4039(00)79366-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012526605"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(70)90146-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013577460"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0021-9673(01)89041-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017261741"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/p19810000538", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023788726"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1058-6687(05)80023-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023792115"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cc800145c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024773811"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cc800145c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024773811"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025529346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tetlet.2004.12.105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026459818"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3011.1991.tb00765.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026642144"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3011.1991.tb00765.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026642144"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/33.8.3894500", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027464746"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/33.8.3894500", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027464746"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2007.07.032", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027995549"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/qsar.200320005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028265520"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2010.01.029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028948189"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2010.01.029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028948189"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(97)89044-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036192514"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cc0600019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039371644"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/cc0600019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039371644"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(03)69017-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045329930"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/354082a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045350492", 
          "https://doi.org/10.1038/354082a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/jcis.1997.5364", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048140096"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1385/0-89603-392-9:1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048639387", 
          "https://doi.org/10.1385/0-89603-392-9:1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0032-9592(93)80023-a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050046819"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bc00029a018", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055153030"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jo01349a529", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1056014823"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3891/acta.chem.scand.33b-0763", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071497811"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014", 
    "datePublishedReg": "2014-01-01", 
    "description": "We describe a method to develop affinity chromatography matrices with short peptide ligands for protein purification. The method entitles the following: (a) synthesis of a combinatorial library on the hydromethylbenzoyl (HMBA)-ChemMatrix resin by the divide-couple-recombine (DCR) method using the Fmoc chemistry, (b) library screening with the protein of interest labeled with a fluorescent dye or biotin, (c) identification of peptides contained on positive beads by tandem matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS/MS), (d) solid-phase peptide ligand synthesis and immobilization in chromatographic supports, and (e) evaluation of protein adsorption on peptide affinity matrices from the equilibrium isotherms and breakthrough curves.", 
    "editor": [
      {
        "familyName": "Labrou", 
        "givenName": "Nikolaos E.", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-62703-977-2_22", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-1-62703-976-5", 
        "978-1-62703-977-2"
      ], 
      "name": "Protein Downstream Processing", 
      "type": "Book"
    }, 
    "name": "Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification", 
    "pagination": "277-302", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-62703-977-2_22"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "2c402cfa6c18e3eb0fb7c5ca4c5f7e13f8ab9c7e7b797cee2f2896ef0c0b672e"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1009289200"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24648083"
        ]
      }
    ], 
    "publisher": {
      "location": "Totowa, NJ", 
      "name": "Humana Press", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-62703-977-2_22", 
      "https://app.dimensions.ai/details/publication/pub.1009289200"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-15T23:50", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8697_00000248.jsonl", 
    "type": "Chapter", 
    "url": "http://link.springer.com/10.1007/978-1-62703-977-2_22"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-977-2_22'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-977-2_22'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-977-2_22'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-977-2_22'


 

This table displays all metadata directly associated to this object as RDF triples.

218 TRIPLES      23 PREDICATES      61 URIs      27 LITERALS      15 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-62703-977-2_22 schema:about N29420128e73b47ec94e68add8e52d4ab
2 N481f60c734b445f2997c21047bffe3c5
3 N607a97db41a0414d9aa9a34cf8f000bc
4 N632e39d97ec64c46a8482d565dbaadef
5 N7e3f4ddbe9f9431f9ff89d90a85240ab
6 Nbe9958a73be34d23ad6143111c2b69f1
7 anzsrc-for:03
8 anzsrc-for:0305
9 schema:author N2f4c474623db4df49ffd9ffb9a505789
10 schema:citation sg:pub.10.1007/0-306-47059-4_7
11 sg:pub.10.1038/354082a0
12 sg:pub.10.1385/0-89603-392-9:1
13 https://doi.org/10.1002/qsar.200320005
14 https://doi.org/10.1006/jcis.1997.5364
15 https://doi.org/10.1016/0003-2697(70)90146-6
16 https://doi.org/10.1016/0003-2697(76)90527-3
17 https://doi.org/10.1016/0032-9592(93)80023-a
18 https://doi.org/10.1016/j.ab.2007.07.032
19 https://doi.org/10.1016/j.ab.2010.01.029
20 https://doi.org/10.1016/j.tetlet.2004.12.105
21 https://doi.org/10.1016/s0021-9673(01)89041-5
22 https://doi.org/10.1016/s0040-4039(00)79366-x
23 https://doi.org/10.1016/s0040-4039(00)88568-8
24 https://doi.org/10.1016/s0076-6879(03)69017-8
25 https://doi.org/10.1016/s0076-6879(97)89038-6
26 https://doi.org/10.1016/s0076-6879(97)89044-1
27 https://doi.org/10.1016/s1058-6687(05)80023-3
28 https://doi.org/10.1021/bc00029a018
29 https://doi.org/10.1021/cc0600019
30 https://doi.org/10.1021/cc800145c
31 https://doi.org/10.1021/jo01349a529
32 https://doi.org/10.1039/b514349g
33 https://doi.org/10.1039/p19810000538
34 https://doi.org/10.1111/j.1399-3011.1991.tb00765.x
35 https://doi.org/10.1177/33.8.3894500
36 https://doi.org/10.3891/acta.chem.scand.33b-0763
37 schema:datePublished 2014
38 schema:datePublishedReg 2014-01-01
39 schema:description We describe a method to develop affinity chromatography matrices with short peptide ligands for protein purification. The method entitles the following: (a) synthesis of a combinatorial library on the hydromethylbenzoyl (HMBA)-ChemMatrix resin by the divide-couple-recombine (DCR) method using the Fmoc chemistry, (b) library screening with the protein of interest labeled with a fluorescent dye or biotin, (c) identification of peptides contained on positive beads by tandem matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS/MS), (d) solid-phase peptide ligand synthesis and immobilization in chromatographic supports, and (e) evaluation of protein adsorption on peptide affinity matrices from the equilibrium isotherms and breakthrough curves.
40 schema:editor N0aa0f84d0d864cb58dbe6bd1fe982cd0
41 schema:genre chapter
42 schema:inLanguage en
43 schema:isAccessibleForFree false
44 schema:isPartOf N5106608d1ee34c1b9e94b40182e072db
45 schema:name Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification
46 schema:pagination 277-302
47 schema:productId N42a215da8a9340e18f33132fed6b48f7
48 N5490dc9e015f478ab0f4d2d97b1397e6
49 N8741608f8d4c4088a916db5c6bbc6883
50 Nbbd1735aefc946f79e732b6256eea63d
51 schema:publisher Nb2ed8ce63072439989f41edad9b862e8
52 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009289200
53 https://doi.org/10.1007/978-1-62703-977-2_22
54 schema:sdDatePublished 2019-04-15T23:50
55 schema:sdLicense https://scigraph.springernature.com/explorer/license/
56 schema:sdPublisher N5b5fb4b68d7d4da2b19052d76b022b1f
57 schema:url http://link.springer.com/10.1007/978-1-62703-977-2_22
58 sgo:license sg:explorer/license/
59 sgo:sdDataset chapters
60 rdf:type schema:Chapter
61 N0aa0f84d0d864cb58dbe6bd1fe982cd0 rdf:first N16a045655dfc47db8569283488cf4d38
62 rdf:rest rdf:nil
63 N16a045655dfc47db8569283488cf4d38 schema:familyName Labrou
64 schema:givenName Nikolaos E.
65 rdf:type schema:Person
66 N29420128e73b47ec94e68add8e52d4ab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
67 schema:name Combinatorial Chemistry Techniques
68 rdf:type schema:DefinedTerm
69 N2f4c474623db4df49ffd9ffb9a505789 rdf:first sg:person.01177030467.82
70 rdf:rest N7956d5e1a45d4048b19aaeefc60e2144
71 N42a215da8a9340e18f33132fed6b48f7 schema:name readcube_id
72 schema:value 2c402cfa6c18e3eb0fb7c5ca4c5f7e13f8ab9c7e7b797cee2f2896ef0c0b672e
73 rdf:type schema:PropertyValue
74 N481f60c734b445f2997c21047bffe3c5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
76 rdf:type schema:DefinedTerm
77 N489c229e128c4670a338d449a4de7171 schema:name Centro Nacional Patagónico-CONICET, Puerto Madryn, Chubut, Argentina
78 rdf:type schema:Organization
79 N5106608d1ee34c1b9e94b40182e072db schema:isbn 978-1-62703-976-5
80 978-1-62703-977-2
81 schema:name Protein Downstream Processing
82 rdf:type schema:Book
83 N5490dc9e015f478ab0f4d2d97b1397e6 schema:name doi
84 schema:value 10.1007/978-1-62703-977-2_22
85 rdf:type schema:PropertyValue
86 N5b5fb4b68d7d4da2b19052d76b022b1f schema:name Springer Nature - SN SciGraph project
87 rdf:type schema:Organization
88 N607a97db41a0414d9aa9a34cf8f000bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Chromatography, Affinity
90 rdf:type schema:DefinedTerm
91 N632e39d97ec64c46a8482d565dbaadef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Peptides
93 rdf:type schema:DefinedTerm
94 N69aaaa4f22db4b50852c47e05d70df25 rdf:first sg:person.01062160206.02
95 rdf:rest rdf:nil
96 N7956d5e1a45d4048b19aaeefc60e2144 rdf:first sg:person.0646206653.02
97 rdf:rest Nbb18c74ca79947c696c211e5e188402e
98 N7e3f4ddbe9f9431f9ff89d90a85240ab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Tandem Mass Spectrometry
100 rdf:type schema:DefinedTerm
101 N8741608f8d4c4088a916db5c6bbc6883 schema:name dimensions_id
102 schema:value pub.1009289200
103 rdf:type schema:PropertyValue
104 Nb2ed8ce63072439989f41edad9b862e8 schema:location Totowa, NJ
105 schema:name Humana Press
106 rdf:type schema:Organisation
107 Nb9d23fc6728f40c49f59b400944bbb79 rdf:first sg:person.01001414756.39
108 rdf:rest N69aaaa4f22db4b50852c47e05d70df25
109 Nbb18c74ca79947c696c211e5e188402e rdf:first sg:person.01270404403.20
110 rdf:rest Nc98547bc07664b05ba70b31c71e10383
111 Nbbd1735aefc946f79e732b6256eea63d schema:name pubmed_id
112 schema:value 24648083
113 rdf:type schema:PropertyValue
114 Nbe9958a73be34d23ad6143111c2b69f1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Proteins
116 rdf:type schema:DefinedTerm
117 Nc98547bc07664b05ba70b31c71e10383 rdf:first sg:person.0767177521.82
118 rdf:rest Nb9d23fc6728f40c49f59b400944bbb79
119 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
120 schema:name Chemical Sciences
121 rdf:type schema:DefinedTerm
122 anzsrc-for:0305 schema:inDefinedTermSet anzsrc-for:
123 schema:name Organic Chemistry
124 rdf:type schema:DefinedTerm
125 sg:person.01001414756.39 schema:affiliation https://www.grid.ac/institutes/grid.5841.8
126 schema:familyName Albericio
127 schema:givenName Fernando
128 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001414756.39
129 rdf:type schema:Person
130 sg:person.01062160206.02 schema:affiliation https://www.grid.ac/institutes/grid.7345.5
131 schema:familyName Cascone
132 schema:givenName Osvaldo
133 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01062160206.02
134 rdf:type schema:Person
135 sg:person.01177030467.82 schema:affiliation https://www.grid.ac/institutes/grid.7345.5
136 schema:familyName Camperi
137 schema:givenName Silvia Andrea
138 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01177030467.82
139 rdf:type schema:Person
140 sg:person.01270404403.20 schema:affiliation https://www.grid.ac/institutes/grid.7345.5
141 schema:familyName Giudicessi
142 schema:givenName Silvana Laura
143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270404403.20
144 rdf:type schema:Person
145 sg:person.0646206653.02 schema:affiliation https://www.grid.ac/institutes/grid.7345.5
146 schema:familyName Martínez-Ceron
147 schema:givenName María Camila
148 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646206653.02
149 rdf:type schema:Person
150 sg:person.0767177521.82 schema:affiliation N489c229e128c4670a338d449a4de7171
151 schema:familyName Marani
152 schema:givenName Mariela Mirta
153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0767177521.82
154 rdf:type schema:Person
155 sg:pub.10.1007/0-306-47059-4_7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010827994
156 https://doi.org/10.1007/0-306-47059-4_7
157 rdf:type schema:CreativeWork
158 sg:pub.10.1038/354082a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045350492
159 https://doi.org/10.1038/354082a0
160 rdf:type schema:CreativeWork
161 sg:pub.10.1385/0-89603-392-9:1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048639387
162 https://doi.org/10.1385/0-89603-392-9:1
163 rdf:type schema:CreativeWork
164 https://doi.org/10.1002/qsar.200320005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028265520
165 rdf:type schema:CreativeWork
166 https://doi.org/10.1006/jcis.1997.5364 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048140096
167 rdf:type schema:CreativeWork
168 https://doi.org/10.1016/0003-2697(70)90146-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013577460
169 rdf:type schema:CreativeWork
170 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1016/0032-9592(93)80023-a schema:sameAs https://app.dimensions.ai/details/publication/pub.1050046819
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1016/j.ab.2007.07.032 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027995549
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1016/j.ab.2010.01.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028948189
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1016/j.tetlet.2004.12.105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026459818
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1016/s0021-9673(01)89041-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017261741
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1016/s0040-4039(00)79366-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1012526605
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1016/s0040-4039(00)88568-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000456847
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1016/s0076-6879(03)69017-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045329930
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1016/s0076-6879(97)89038-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002270197
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/s0076-6879(97)89044-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036192514
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/s1058-6687(05)80023-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023792115
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1021/bc00029a018 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055153030
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1021/cc0600019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039371644
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1021/cc800145c schema:sameAs https://app.dimensions.ai/details/publication/pub.1024773811
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1021/jo01349a529 schema:sameAs https://app.dimensions.ai/details/publication/pub.1056014823
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1039/b514349g schema:sameAs https://app.dimensions.ai/details/publication/pub.1011648275
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1039/p19810000538 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023788726
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1111/j.1399-3011.1991.tb00765.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1026642144
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1177/33.8.3894500 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027464746
209 rdf:type schema:CreativeWork
210 https://doi.org/10.3891/acta.chem.scand.33b-0763 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071497811
211 rdf:type schema:CreativeWork
212 https://www.grid.ac/institutes/grid.5841.8 schema:alternateName University of Barcelona
213 schema:name Instituto de Investigaciones Biomédicas, Parque Científico de Barcelona, Universidad de Barcelona, Barcelona, Spain
214 rdf:type schema:Organization
215 https://www.grid.ac/institutes/grid.7345.5 schema:alternateName University of Buenos Aires
216 schema:name Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
217 Institute of Nanobiotechnology UBA-CONICET, Cathedra of Industrial Microbiology and Biotechnology, School of Pharmacy and Biochemistry, University of Buenos Aires, Junín 956, C1113AAD, Buenos Aires, Argentina
218 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...