A Yeast-Based Recombination Assay for Homing Endonuclease Activity View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2014

AUTHORS

Jean-Charles Epinat

ABSTRACT

Homing endonucleases (HEs) are natural enzymes that cleave long DNA target with a high specificity and trigger homologous recombination at the exact site of the break. Such mechanisms can thus be used for all the applications covered today by the generic name of "genome engineering": targeted sequence insertion, removal, or editing. However, before being able to address those applications, the engineering of HEs must be mastered so that any potential target would be efficiently and specifically recognized and cleaved. Working on the I-CreI model, we have developed a very powerful platform to generate HEs with new tailored specificity. We have put in place the first in vivo, functional, high throughput assay to generate I-CreI variants and measure their activity. We use semi-rational design combined with proprietary in silico predictions to design and synthesize I-CreI mutants that are tested for their capacity to induce homologous recombination in a yeast cell. The process has been standardized and robotized so that we can generate thousands of I-CreI derivatives, characterize their cleavage profile, and deliver them for further applications in the research, therapeutic, or agrobusiness fields. More... »

PAGES

105-26

References to SciGraph publications

  • 1997-05. The first genome from the third domain of life in NATURE
  • 2013. Engineered Meganucleases for Genome Engineering Purposes in SITE-DIRECTED INSERTION OF TRANSGENES
  • 2008. Triplex-Mediated Gene Modification in CHROMOSOMAL MUTAGENESIS
  • 2013. Bio-applications Derived from Site-Directed Genome Modification Technologies in SITE-DIRECTED INSERTION OF TRANSGENES
  • 2013. Engineered Zinc Finger Nucleases for Targeted Genome Editing in SITE-DIRECTED INSERTION OF TRANSGENES
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/978-1-62703-968-0_9

    DOI

    http://dx.doi.org/10.1007/978-1-62703-968-0_9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1026980450

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24510264


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA Restriction Enzymes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Endonucleases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "High-Throughput Screening Assays", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Homologous Recombination", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Yeasts", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Takara (France)", 
              "id": "https://www.grid.ac/institutes/grid.433267.7", 
              "name": [
                "Cellectis SA, Paris, France."
              ], 
              "type": "Organization"
            }, 
            "familyName": "Epinat", 
            "givenName": "Jean-Charles", 
            "id": "sg:person.0643220465.52", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643220465.52"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1093/nar/gkl645", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000602953"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0092-8674(85)80011-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001996746"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(89)90035-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002727102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(89)90035-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002727102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jaci.2011.02.027", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003812506"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/genetics.110.120717", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007578180"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1534/genetics.110.120717", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007578180"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/1087057112441623", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008281264"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/1087057112441623", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008281264"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gni148", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008552863"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0076-6879(02)50957-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010540893"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gks320", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012705120"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkf495", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014225506"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jmb.2003.10.013", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014343686"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gks226", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014494291"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jmb.2004.07.031", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014507230"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkg375", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016134561"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1139/g2012-049", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018804794"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkr186", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018933772"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkn1007", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020628626"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/1087057106296498", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021649672"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/1087057106296498", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021649672"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jmb.2007.04.079", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022911216"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-94-007-4531-5_5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1027512856", 
              "https://doi.org/10.1007/978-94-007-4531-5_5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-94-007-4531-5_6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028090798", 
              "https://doi.org/10.1007/978-94-007-4531-5_6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/25.17.3379", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030001797"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0076-6879(91)94004-v", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031136115"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jmb.2005.10.065", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032155186"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jmb.2005.10.065", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032155186"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-94-007-4531-5_14", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032281943", 
              "https://doi.org/10.1007/978-94-007-4531-5_14"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gks268", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032715859"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.str.2010.12.003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033555246"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-59745-232-8_13", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035082448", 
              "https://doi.org/10.1007/978-1-59745-232-8_13"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-59745-232-8_13", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035082448", 
              "https://doi.org/10.1007/978-1-59745-232-8_13"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.270.51.30237", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036770276"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1567-1364.2006.00204.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036915047"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkp548", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037034816"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/108705719900400206", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038278323"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/108705719900400206", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038278323"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkp726", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038920901"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkm182", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039608420"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(83)90219-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040207950"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/1087057103258284", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041006599"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/1087057103258284", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041006599"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/22.15.3241", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041299239"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkp1171", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041612079"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/29.18.3757", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043449639"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/30.7.e29", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043929402"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/protein/gzp001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044988978"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/protein/gzq083", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046099390"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/protein/gzq083", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046099390"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gni175", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046178868"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2174/156652311794520111", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046423203"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/gkl720", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046669585"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m706323200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048372035"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/1087057103258285", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049958189"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1177/1087057103258285", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049958189"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.3109/10409238.2011.652358", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051697385"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/387459a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052426351", 
              "https://doi.org/10.1038/387459a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ja057519l", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055840788"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ja057519l", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055840788"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.2174/156652307779940216", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1069184444"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1074471852", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079262726", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/ajhp/46.1.104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1079262726"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/ajhp/46.1.104", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1079262726"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2014", 
        "datePublishedReg": "2014-01-01", 
        "description": "Homing endonucleases (HEs) are natural enzymes that cleave long DNA target with a high specificity and trigger homologous recombination at the exact site of the break. Such mechanisms can thus be used for all the applications covered today by the generic name of \"genome engineering\": targeted sequence insertion, removal, or editing. However, before being able to address those applications, the engineering of HEs must be mastered so that any potential target would be efficiently and specifically recognized and cleaved. Working on the I-CreI model, we have developed a very powerful platform to generate HEs with new tailored specificity. We have put in place the first in vivo, functional, high throughput assay to generate I-CreI variants and measure their activity. We use semi-rational design combined with proprietary in silico predictions to design and synthesize I-CreI mutants that are tested for their capacity to induce homologous recombination in a yeast cell. The process has been standardized and robotized so that we can generate thousands of I-CreI derivatives, characterize their cleavage profile, and deliver them for further applications in the research, therapeutic, or agrobusiness fields. ", 
        "editor": [
          {
            "familyName": "Edgell", 
            "givenName": "David R.", 
            "type": "Person"
          }
        ], 
        "genre": "chapter", 
        "id": "sg:pub.10.1007/978-1-62703-968-0_9", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": {
          "isbn": [
            "978-1-62703-967-3", 
            "978-1-62703-968-0"
          ], 
          "name": "Homing Endonucleases", 
          "type": "Book"
        }, 
        "name": "A Yeast-Based Recombination Assay for Homing Endonuclease Activity", 
        "pagination": "105-26", 
        "productId": [
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/978-1-62703-968-0_9"
            ]
          }, 
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "80874d2eaf52d47a1d5212558857bc2ee726ecadef10c54d49c44a12afa806a7"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1026980450"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "24510264"
            ]
          }
        ], 
        "publisher": {
          "location": "Totowa, NJ", 
          "name": "Humana Press", 
          "type": "Organisation"
        }, 
        "sameAs": [
          "https://doi.org/10.1007/978-1-62703-968-0_9", 
          "https://app.dimensions.ai/details/publication/pub.1026980450"
        ], 
        "sdDataset": "chapters", 
        "sdDatePublished": "2019-04-15T11:34", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000259.jsonl", 
        "type": "Chapter", 
        "url": "http://link.springer.com/10.1007/978-1-62703-968-0_9"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-968-0_9'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-968-0_9'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-968-0_9'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-968-0_9'


     

    This table displays all metadata directly associated to this object as RDF triples.

    254 TRIPLES      23 PREDICATES      87 URIs      26 LITERALS      14 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/978-1-62703-968-0_9 schema:about N050d2f9a717041bdbd3ccfbbf5518c7c
    2 N2cc7a845a85e41bd86ffd919b87903f4
    3 N8ffa1319f32149fd8063dd3d28426a08
    4 Nda66b0482f9f40b3831d2ecefb56382f
    5 Nddd2b928e756494cac4aff17126062f9
    6 anzsrc-for:06
    7 anzsrc-for:0601
    8 schema:author N0e06c9de1d2c4a739a98b3714e0af9e7
    9 schema:citation sg:pub.10.1007/978-1-59745-232-8_13
    10 sg:pub.10.1007/978-94-007-4531-5_14
    11 sg:pub.10.1007/978-94-007-4531-5_5
    12 sg:pub.10.1007/978-94-007-4531-5_6
    13 sg:pub.10.1038/387459a0
    14 https://app.dimensions.ai/details/publication/pub.1074471852
    15 https://app.dimensions.ai/details/publication/pub.1079262726
    16 https://doi.org/10.1016/0076-6879(91)94004-v
    17 https://doi.org/10.1016/0092-8674(83)90219-2
    18 https://doi.org/10.1016/0378-1119(89)90035-8
    19 https://doi.org/10.1016/j.jaci.2011.02.027
    20 https://doi.org/10.1016/j.jmb.2003.10.013
    21 https://doi.org/10.1016/j.jmb.2004.07.031
    22 https://doi.org/10.1016/j.jmb.2005.10.065
    23 https://doi.org/10.1016/j.jmb.2007.04.079
    24 https://doi.org/10.1016/j.str.2010.12.003
    25 https://doi.org/10.1016/s0076-6879(02)50957-5
    26 https://doi.org/10.1016/s0092-8674(85)80011-8
    27 https://doi.org/10.1021/ja057519l
    28 https://doi.org/10.1074/jbc.270.51.30237
    29 https://doi.org/10.1074/jbc.m706323200
    30 https://doi.org/10.1093/ajhp/46.1.104
    31 https://doi.org/10.1093/nar/22.15.3241
    32 https://doi.org/10.1093/nar/25.17.3379
    33 https://doi.org/10.1093/nar/29.18.3757
    34 https://doi.org/10.1093/nar/30.7.e29
    35 https://doi.org/10.1093/nar/gkf495
    36 https://doi.org/10.1093/nar/gkg375
    37 https://doi.org/10.1093/nar/gkl645
    38 https://doi.org/10.1093/nar/gkl720
    39 https://doi.org/10.1093/nar/gkm182
    40 https://doi.org/10.1093/nar/gkn1007
    41 https://doi.org/10.1093/nar/gkp1171
    42 https://doi.org/10.1093/nar/gkp548
    43 https://doi.org/10.1093/nar/gkp726
    44 https://doi.org/10.1093/nar/gkr186
    45 https://doi.org/10.1093/nar/gks226
    46 https://doi.org/10.1093/nar/gks268
    47 https://doi.org/10.1093/nar/gks320
    48 https://doi.org/10.1093/nar/gni148
    49 https://doi.org/10.1093/nar/gni175
    50 https://doi.org/10.1093/protein/gzp001
    51 https://doi.org/10.1093/protein/gzq083
    52 https://doi.org/10.1111/j.1567-1364.2006.00204.x
    53 https://doi.org/10.1139/g2012-049
    54 https://doi.org/10.1177/1087057103258284
    55 https://doi.org/10.1177/1087057103258285
    56 https://doi.org/10.1177/1087057106296498
    57 https://doi.org/10.1177/1087057112441623
    58 https://doi.org/10.1177/108705719900400206
    59 https://doi.org/10.1534/genetics.110.120717
    60 https://doi.org/10.2174/156652307779940216
    61 https://doi.org/10.2174/156652311794520111
    62 https://doi.org/10.3109/10409238.2011.652358
    63 schema:datePublished 2014
    64 schema:datePublishedReg 2014-01-01
    65 schema:description Homing endonucleases (HEs) are natural enzymes that cleave long DNA target with a high specificity and trigger homologous recombination at the exact site of the break. Such mechanisms can thus be used for all the applications covered today by the generic name of "genome engineering": targeted sequence insertion, removal, or editing. However, before being able to address those applications, the engineering of HEs must be mastered so that any potential target would be efficiently and specifically recognized and cleaved. Working on the I-CreI model, we have developed a very powerful platform to generate HEs with new tailored specificity. We have put in place the first in vivo, functional, high throughput assay to generate I-CreI variants and measure their activity. We use semi-rational design combined with proprietary in silico predictions to design and synthesize I-CreI mutants that are tested for their capacity to induce homologous recombination in a yeast cell. The process has been standardized and robotized so that we can generate thousands of I-CreI derivatives, characterize their cleavage profile, and deliver them for further applications in the research, therapeutic, or agrobusiness fields.
    66 schema:editor N3cab771e5c1449399aaf79102c57008e
    67 schema:genre chapter
    68 schema:inLanguage en
    69 schema:isAccessibleForFree false
    70 schema:isPartOf N795883de3db0468e8eafbd5f4059b0b9
    71 schema:name A Yeast-Based Recombination Assay for Homing Endonuclease Activity
    72 schema:pagination 105-26
    73 schema:productId N0ea65c74a0f946869a4995430704a0f4
    74 N195719fd0a664252b0d53b554b59b58e
    75 N5f8d988d783944d0b05140a6c2947a49
    76 Nf16b67dd7995484598514b8f05315108
    77 schema:publisher N9eeb9311c2e84abeab30156df7ad6783
    78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026980450
    79 https://doi.org/10.1007/978-1-62703-968-0_9
    80 schema:sdDatePublished 2019-04-15T11:34
    81 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    82 schema:sdPublisher Nd768ec6d70904b8590f6cce1bc9dd380
    83 schema:url http://link.springer.com/10.1007/978-1-62703-968-0_9
    84 sgo:license sg:explorer/license/
    85 sgo:sdDataset chapters
    86 rdf:type schema:Chapter
    87 N050d2f9a717041bdbd3ccfbbf5518c7c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    88 schema:name Yeasts
    89 rdf:type schema:DefinedTerm
    90 N0e06c9de1d2c4a739a98b3714e0af9e7 rdf:first sg:person.0643220465.52
    91 rdf:rest rdf:nil
    92 N0ea65c74a0f946869a4995430704a0f4 schema:name doi
    93 schema:value 10.1007/978-1-62703-968-0_9
    94 rdf:type schema:PropertyValue
    95 N195719fd0a664252b0d53b554b59b58e schema:name pubmed_id
    96 schema:value 24510264
    97 rdf:type schema:PropertyValue
    98 N2cc7a845a85e41bd86ffd919b87903f4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name High-Throughput Screening Assays
    100 rdf:type schema:DefinedTerm
    101 N3cab771e5c1449399aaf79102c57008e rdf:first N966d875865554e5d92385f0ed3877a85
    102 rdf:rest rdf:nil
    103 N5f8d988d783944d0b05140a6c2947a49 schema:name dimensions_id
    104 schema:value pub.1026980450
    105 rdf:type schema:PropertyValue
    106 N795883de3db0468e8eafbd5f4059b0b9 schema:isbn 978-1-62703-967-3
    107 978-1-62703-968-0
    108 schema:name Homing Endonucleases
    109 rdf:type schema:Book
    110 N8ffa1319f32149fd8063dd3d28426a08 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name Homologous Recombination
    112 rdf:type schema:DefinedTerm
    113 N966d875865554e5d92385f0ed3877a85 schema:familyName Edgell
    114 schema:givenName David R.
    115 rdf:type schema:Person
    116 N9eeb9311c2e84abeab30156df7ad6783 schema:location Totowa, NJ
    117 schema:name Humana Press
    118 rdf:type schema:Organisation
    119 Nd768ec6d70904b8590f6cce1bc9dd380 schema:name Springer Nature - SN SciGraph project
    120 rdf:type schema:Organization
    121 Nda66b0482f9f40b3831d2ecefb56382f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name DNA Restriction Enzymes
    123 rdf:type schema:DefinedTerm
    124 Nddd2b928e756494cac4aff17126062f9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    125 schema:name Endonucleases
    126 rdf:type schema:DefinedTerm
    127 Nf16b67dd7995484598514b8f05315108 schema:name readcube_id
    128 schema:value 80874d2eaf52d47a1d5212558857bc2ee726ecadef10c54d49c44a12afa806a7
    129 rdf:type schema:PropertyValue
    130 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    131 schema:name Biological Sciences
    132 rdf:type schema:DefinedTerm
    133 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    134 schema:name Biochemistry and Cell Biology
    135 rdf:type schema:DefinedTerm
    136 sg:person.0643220465.52 schema:affiliation https://www.grid.ac/institutes/grid.433267.7
    137 schema:familyName Epinat
    138 schema:givenName Jean-Charles
    139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643220465.52
    140 rdf:type schema:Person
    141 sg:pub.10.1007/978-1-59745-232-8_13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035082448
    142 https://doi.org/10.1007/978-1-59745-232-8_13
    143 rdf:type schema:CreativeWork
    144 sg:pub.10.1007/978-94-007-4531-5_14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032281943
    145 https://doi.org/10.1007/978-94-007-4531-5_14
    146 rdf:type schema:CreativeWork
    147 sg:pub.10.1007/978-94-007-4531-5_5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027512856
    148 https://doi.org/10.1007/978-94-007-4531-5_5
    149 rdf:type schema:CreativeWork
    150 sg:pub.10.1007/978-94-007-4531-5_6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028090798
    151 https://doi.org/10.1007/978-94-007-4531-5_6
    152 rdf:type schema:CreativeWork
    153 sg:pub.10.1038/387459a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052426351
    154 https://doi.org/10.1038/387459a0
    155 rdf:type schema:CreativeWork
    156 https://app.dimensions.ai/details/publication/pub.1074471852 schema:CreativeWork
    157 https://app.dimensions.ai/details/publication/pub.1079262726 schema:CreativeWork
    158 https://doi.org/10.1016/0076-6879(91)94004-v schema:sameAs https://app.dimensions.ai/details/publication/pub.1031136115
    159 rdf:type schema:CreativeWork
    160 https://doi.org/10.1016/0092-8674(83)90219-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040207950
    161 rdf:type schema:CreativeWork
    162 https://doi.org/10.1016/0378-1119(89)90035-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002727102
    163 rdf:type schema:CreativeWork
    164 https://doi.org/10.1016/j.jaci.2011.02.027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003812506
    165 rdf:type schema:CreativeWork
    166 https://doi.org/10.1016/j.jmb.2003.10.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014343686
    167 rdf:type schema:CreativeWork
    168 https://doi.org/10.1016/j.jmb.2004.07.031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014507230
    169 rdf:type schema:CreativeWork
    170 https://doi.org/10.1016/j.jmb.2005.10.065 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032155186
    171 rdf:type schema:CreativeWork
    172 https://doi.org/10.1016/j.jmb.2007.04.079 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022911216
    173 rdf:type schema:CreativeWork
    174 https://doi.org/10.1016/j.str.2010.12.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033555246
    175 rdf:type schema:CreativeWork
    176 https://doi.org/10.1016/s0076-6879(02)50957-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010540893
    177 rdf:type schema:CreativeWork
    178 https://doi.org/10.1016/s0092-8674(85)80011-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001996746
    179 rdf:type schema:CreativeWork
    180 https://doi.org/10.1021/ja057519l schema:sameAs https://app.dimensions.ai/details/publication/pub.1055840788
    181 rdf:type schema:CreativeWork
    182 https://doi.org/10.1074/jbc.270.51.30237 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036770276
    183 rdf:type schema:CreativeWork
    184 https://doi.org/10.1074/jbc.m706323200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048372035
    185 rdf:type schema:CreativeWork
    186 https://doi.org/10.1093/ajhp/46.1.104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1079262726
    187 rdf:type schema:CreativeWork
    188 https://doi.org/10.1093/nar/22.15.3241 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041299239
    189 rdf:type schema:CreativeWork
    190 https://doi.org/10.1093/nar/25.17.3379 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030001797
    191 rdf:type schema:CreativeWork
    192 https://doi.org/10.1093/nar/29.18.3757 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043449639
    193 rdf:type schema:CreativeWork
    194 https://doi.org/10.1093/nar/30.7.e29 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043929402
    195 rdf:type schema:CreativeWork
    196 https://doi.org/10.1093/nar/gkf495 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014225506
    197 rdf:type schema:CreativeWork
    198 https://doi.org/10.1093/nar/gkg375 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016134561
    199 rdf:type schema:CreativeWork
    200 https://doi.org/10.1093/nar/gkl645 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000602953
    201 rdf:type schema:CreativeWork
    202 https://doi.org/10.1093/nar/gkl720 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046669585
    203 rdf:type schema:CreativeWork
    204 https://doi.org/10.1093/nar/gkm182 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039608420
    205 rdf:type schema:CreativeWork
    206 https://doi.org/10.1093/nar/gkn1007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020628626
    207 rdf:type schema:CreativeWork
    208 https://doi.org/10.1093/nar/gkp1171 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041612079
    209 rdf:type schema:CreativeWork
    210 https://doi.org/10.1093/nar/gkp548 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037034816
    211 rdf:type schema:CreativeWork
    212 https://doi.org/10.1093/nar/gkp726 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038920901
    213 rdf:type schema:CreativeWork
    214 https://doi.org/10.1093/nar/gkr186 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018933772
    215 rdf:type schema:CreativeWork
    216 https://doi.org/10.1093/nar/gks226 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014494291
    217 rdf:type schema:CreativeWork
    218 https://doi.org/10.1093/nar/gks268 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032715859
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1093/nar/gks320 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012705120
    221 rdf:type schema:CreativeWork
    222 https://doi.org/10.1093/nar/gni148 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008552863
    223 rdf:type schema:CreativeWork
    224 https://doi.org/10.1093/nar/gni175 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046178868
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1093/protein/gzp001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044988978
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1093/protein/gzq083 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046099390
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1111/j.1567-1364.2006.00204.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1036915047
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1139/g2012-049 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018804794
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1177/1087057103258284 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041006599
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1177/1087057103258285 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049958189
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1177/1087057106296498 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021649672
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1177/1087057112441623 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008281264
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1177/108705719900400206 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038278323
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1534/genetics.110.120717 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007578180
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.2174/156652307779940216 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069184444
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.2174/156652311794520111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046423203
    249 rdf:type schema:CreativeWork
    250 https://doi.org/10.3109/10409238.2011.652358 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051697385
    251 rdf:type schema:CreativeWork
    252 https://www.grid.ac/institutes/grid.433267.7 schema:alternateName Takara (France)
    253 schema:name Cellectis SA, Paris, France.
    254 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...