Preparation and Application of Triple Helix Forming Oligonucleotides and Single Strand Oligonucleotide Donors for Gene Correction View Full Text


Ontology type: schema:Chapter      Open Access: True


Chapter Info

DATE

2014

AUTHORS

Rowshon Alam , Arun Kalliat Thazhathveetil , Hong Li , Michael M. Seidman

ABSTRACT

Strategies for site-specific modulation of genomic sequences in mammalian cells require two components. One must be capable of recognizing and activating a specific target sequence in vivo, driving that site into an exploitable repair pathway. Information is transferred to the site via participation in the pathway by the second component, a donor nucleic acid, resulting in a permanent change in the target sequence. We have developed biologically active triple helix forming oligonucleotides (TFOs) as site-specific gene targeting reagents. These TFOs, linked to DNA reactive compounds (such as a cross-linking agent), activate pathways that can engage informational donors. We have used the combination of a psoralen-TFO and single strand oligonucleotide donors to generate novel cell lines with directed sequence changes at the target site. Here we describe the synthesis and purification of bioactive psoralen-linked TFOs, their co-introduction into mammalian cells with donor nucleic acids, and the identification of cells with sequence conversion of the target site. We have emphasized details in the synthesis and purification of the oligonucleotides that are essential for preparation of reagents with optimal activity. More... »

PAGES

103-13

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-62703-761-7_7

DOI

http://dx.doi.org/10.1007/978-1-62703-761-7_7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029928838

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24557899


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CHO Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Culture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cricetulus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Electroporation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Targeting", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oligonucleotides", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Girindus America, Inc., Cincinnati, OH, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Alam", 
        "givenName": "Rowshon", 
        "id": "sg:person.01216734772.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216734772.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Northwestern University", 
          "id": "https://www.grid.ac/institutes/grid.16753.36", 
          "name": [
            "Department of Chemistry, Northwestern University, Evanston, IL, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thazhathveetil", 
        "givenName": "Arun Kalliat", 
        "id": "sg:person.01334560047.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01334560047.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Lasergen (United States)", 
          "id": "https://www.grid.ac/institutes/grid.420805.b", 
          "name": [
            "LaserGen, Inc, Houston, TX, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Hong", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute on Aging", 
          "id": "https://www.grid.ac/institutes/grid.419475.a", 
          "name": [
            "National Institute on Aging (NIA), NIH, Baltimore, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Seidman", 
        "givenName": "Michael M.", 
        "id": "sg:person.01230543023.58", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230543023.58"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1002/anie.199306661", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002833413"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nass/44.1.241", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013687255"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/2530", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016425762", 
          "https://doi.org/10.1038/2530"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/2530", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016425762", 
          "https://doi.org/10.1038/2530"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m110.123844", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019782748"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0959-440x(96)80051-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020303860"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.16.7879", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038360632"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.88.13.5602", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041380226"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci19552", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042034032"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m800607200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043804600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-894x(98)00366-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047543664"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/12.16.6603", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048404500"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bc900008s", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055155720"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bc900008s", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055155720"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi025734y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055195763"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi025734y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055195763"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi035808l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055198049"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi035808l", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055198049"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0520986", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055201296"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi0520986", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055201296"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9816352", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055216419"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi9816352", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055216419"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja00010a077", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055698800"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja01565a074", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055808043"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1279808", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062471453"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.271.5250.802", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062552199"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075372021", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014", 
    "datePublishedReg": "2014-01-01", 
    "description": "Strategies for site-specific modulation of genomic sequences in mammalian cells require two components. One must be capable of recognizing and activating a specific target sequence in vivo, driving that site into an exploitable repair pathway. Information is transferred to the site via participation in the pathway by the second component, a donor nucleic acid, resulting in a permanent change in the target sequence. We have developed biologically active triple helix forming oligonucleotides (TFOs) as site-specific gene targeting reagents. These TFOs, linked to DNA reactive compounds (such as a cross-linking agent), activate pathways that can engage informational donors. We have used the combination of a psoralen-TFO and single strand oligonucleotide donors to generate novel cell lines with directed sequence changes at the target site. Here we describe the synthesis and purification of bioactive psoralen-linked TFOs, their co-introduction into mammalian cells with donor nucleic acids, and the identification of cells with sequence conversion of the target site. We have emphasized details in the synthesis and purification of the oligonucleotides that are essential for preparation of reagents with optimal activity.", 
    "editor": [
      {
        "familyName": "Storici", 
        "givenName": "Francesca", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-62703-761-7_7", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2722857", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": {
      "isbn": [
        "978-1-62703-760-0", 
        "978-1-62703-761-7"
      ], 
      "name": "Gene Correction", 
      "type": "Book"
    }, 
    "name": "Preparation and Application of Triple Helix Forming Oligonucleotides and Single Strand Oligonucleotide Donors for Gene Correction", 
    "pagination": "103-13", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-62703-761-7_7"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "8e93b708e9c919f79a5e07fc0517433cc1a6ee4ae47cfcfe066893139e170db0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1029928838"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24557899"
        ]
      }
    ], 
    "publisher": {
      "location": "Totowa, NJ", 
      "name": "Humana Press", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-62703-761-7_7", 
      "https://app.dimensions.ai/details/publication/pub.1029928838"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-15T16:27", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8675_00000353.jsonl", 
    "type": "Chapter", 
    "url": "http://link.springer.com/10.1007/978-1-62703-761-7_7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-761-7_7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-761-7_7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-761-7_7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-761-7_7'


 

This table displays all metadata directly associated to this object as RDF triples.

202 TRIPLES      23 PREDICATES      59 URIs      31 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-62703-761-7_7 schema:about N0ca6682fcea343d597196f435cde3756
2 N2674a393f74848f2abf1c4a4496f81b5
3 N42a6250c451d4cc182b8402b6aa24580
4 N48e71badb79147ac95d875b4839b5d2c
5 N6e4c0c8b58c8433eb9b32448a75583f7
6 Nc9795005a75d4ef69dc75a895e921ce7
7 Ncc002084ef164a0f80015d8d7a705ead
8 Nd64289f5c3d44f97b82f05d068a44fa5
9 Nfb3e1ac6d0d349ffa323f2d8f6a8dbed
10 Nfc1bf5f6f8a14bfc8739df9695297127
11 anzsrc-for:06
12 anzsrc-for:0601
13 schema:author N16dddd7d03244e468d0a65c6d2857025
14 schema:citation sg:pub.10.1038/2530
15 https://app.dimensions.ai/details/publication/pub.1075372021
16 https://doi.org/10.1002/anie.199306661
17 https://doi.org/10.1016/s0959-440x(96)80051-0
18 https://doi.org/10.1016/s0960-894x(98)00366-7
19 https://doi.org/10.1021/bc900008s
20 https://doi.org/10.1021/bi025734y
21 https://doi.org/10.1021/bi035808l
22 https://doi.org/10.1021/bi0520986
23 https://doi.org/10.1021/bi9816352
24 https://doi.org/10.1021/ja00010a077
25 https://doi.org/10.1021/ja01565a074
26 https://doi.org/10.1073/pnas.88.13.5602
27 https://doi.org/10.1073/pnas.90.16.7879
28 https://doi.org/10.1074/jbc.m110.123844
29 https://doi.org/10.1074/jbc.m800607200
30 https://doi.org/10.1093/nar/12.16.6603
31 https://doi.org/10.1093/nass/44.1.241
32 https://doi.org/10.1126/science.1279808
33 https://doi.org/10.1126/science.271.5250.802
34 https://doi.org/10.1172/jci19552
35 schema:datePublished 2014
36 schema:datePublishedReg 2014-01-01
37 schema:description Strategies for site-specific modulation of genomic sequences in mammalian cells require two components. One must be capable of recognizing and activating a specific target sequence in vivo, driving that site into an exploitable repair pathway. Information is transferred to the site via participation in the pathway by the second component, a donor nucleic acid, resulting in a permanent change in the target sequence. We have developed biologically active triple helix forming oligonucleotides (TFOs) as site-specific gene targeting reagents. These TFOs, linked to DNA reactive compounds (such as a cross-linking agent), activate pathways that can engage informational donors. We have used the combination of a psoralen-TFO and single strand oligonucleotide donors to generate novel cell lines with directed sequence changes at the target site. Here we describe the synthesis and purification of bioactive psoralen-linked TFOs, their co-introduction into mammalian cells with donor nucleic acids, and the identification of cells with sequence conversion of the target site. We have emphasized details in the synthesis and purification of the oligonucleotides that are essential for preparation of reagents with optimal activity.
38 schema:editor N4c643e2a592348528cee05d54b77527d
39 schema:genre chapter
40 schema:inLanguage en
41 schema:isAccessibleForFree true
42 schema:isPartOf N0709433274334df6a4260181788306b0
43 schema:name Preparation and Application of Triple Helix Forming Oligonucleotides and Single Strand Oligonucleotide Donors for Gene Correction
44 schema:pagination 103-13
45 schema:productId N558f8a31e0094f51a4f5f27f5380dddf
46 N5c742999755c4be4b8341d63d71e948b
47 Nb11b4659a0b04014beb3bea242cfff8c
48 Nd39b9124ea184a0e8ce5492d336b7988
49 schema:publisher N9d9724072efe4b0ab8fc1b9adc60167b
50 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029928838
51 https://doi.org/10.1007/978-1-62703-761-7_7
52 schema:sdDatePublished 2019-04-15T16:27
53 schema:sdLicense https://scigraph.springernature.com/explorer/license/
54 schema:sdPublisher N57cee4f3fbd04dea96fff6d0c9df3b65
55 schema:url http://link.springer.com/10.1007/978-1-62703-761-7_7
56 sgo:license sg:explorer/license/
57 sgo:sdDataset chapters
58 rdf:type schema:Chapter
59 N02440f4ca2cb40a5a9275eaf8c77c33e rdf:first sg:person.01230543023.58
60 rdf:rest rdf:nil
61 N06d60fa07b3f4d4183bf7370adfb6428 rdf:first sg:person.01334560047.39
62 rdf:rest Nacd0645b208a4e7583b499744ae1a64c
63 N0709433274334df6a4260181788306b0 schema:isbn 978-1-62703-760-0
64 978-1-62703-761-7
65 schema:name Gene Correction
66 rdf:type schema:Book
67 N0ca6682fcea343d597196f435cde3756 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Humans
69 rdf:type schema:DefinedTerm
70 N16dddd7d03244e468d0a65c6d2857025 rdf:first sg:person.01216734772.20
71 rdf:rest N06d60fa07b3f4d4183bf7370adfb6428
72 N2674a393f74848f2abf1c4a4496f81b5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
73 schema:name Cell Line
74 rdf:type schema:DefinedTerm
75 N42a6250c451d4cc182b8402b6aa24580 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Electroporation
77 rdf:type schema:DefinedTerm
78 N48e71badb79147ac95d875b4839b5d2c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name CHO Cells
80 rdf:type schema:DefinedTerm
81 N4c643e2a592348528cee05d54b77527d rdf:first Nf3a40aeef95e438e9c4c3f2cf7a3dd31
82 rdf:rest rdf:nil
83 N558f8a31e0094f51a4f5f27f5380dddf schema:name pubmed_id
84 schema:value 24557899
85 rdf:type schema:PropertyValue
86 N57cee4f3fbd04dea96fff6d0c9df3b65 schema:name Springer Nature - SN SciGraph project
87 rdf:type schema:Organization
88 N5868ea9ea7c7420faf8181671a8d9192 schema:name Girindus America, Inc., Cincinnati, OH, USA
89 rdf:type schema:Organization
90 N5c742999755c4be4b8341d63d71e948b schema:name readcube_id
91 schema:value 8e93b708e9c919f79a5e07fc0517433cc1a6ee4ae47cfcfe066893139e170db0
92 rdf:type schema:PropertyValue
93 N6e4c0c8b58c8433eb9b32448a75583f7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Cricetulus
95 rdf:type schema:DefinedTerm
96 N7a6235a10a5b4e75bdd6f065cd973ed7 schema:affiliation https://www.grid.ac/institutes/grid.420805.b
97 schema:familyName Li
98 schema:givenName Hong
99 rdf:type schema:Person
100 N9d9724072efe4b0ab8fc1b9adc60167b schema:location Totowa, NJ
101 schema:name Humana Press
102 rdf:type schema:Organisation
103 Nacd0645b208a4e7583b499744ae1a64c rdf:first N7a6235a10a5b4e75bdd6f065cd973ed7
104 rdf:rest N02440f4ca2cb40a5a9275eaf8c77c33e
105 Nb11b4659a0b04014beb3bea242cfff8c schema:name doi
106 schema:value 10.1007/978-1-62703-761-7_7
107 rdf:type schema:PropertyValue
108 Nc9795005a75d4ef69dc75a895e921ce7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Gene Expression
110 rdf:type schema:DefinedTerm
111 Ncc002084ef164a0f80015d8d7a705ead schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Oligonucleotides
113 rdf:type schema:DefinedTerm
114 Nd39b9124ea184a0e8ce5492d336b7988 schema:name dimensions_id
115 schema:value pub.1029928838
116 rdf:type schema:PropertyValue
117 Nd64289f5c3d44f97b82f05d068a44fa5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Animals
119 rdf:type schema:DefinedTerm
120 Nf3a40aeef95e438e9c4c3f2cf7a3dd31 schema:familyName Storici
121 schema:givenName Francesca
122 rdf:type schema:Person
123 Nfb3e1ac6d0d349ffa323f2d8f6a8dbed schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Cell Culture Techniques
125 rdf:type schema:DefinedTerm
126 Nfc1bf5f6f8a14bfc8739df9695297127 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Gene Targeting
128 rdf:type schema:DefinedTerm
129 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
130 schema:name Biological Sciences
131 rdf:type schema:DefinedTerm
132 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
133 schema:name Biochemistry and Cell Biology
134 rdf:type schema:DefinedTerm
135 sg:grant.2722857 http://pending.schema.org/fundedItem sg:pub.10.1007/978-1-62703-761-7_7
136 rdf:type schema:MonetaryGrant
137 sg:person.01216734772.20 schema:affiliation N5868ea9ea7c7420faf8181671a8d9192
138 schema:familyName Alam
139 schema:givenName Rowshon
140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216734772.20
141 rdf:type schema:Person
142 sg:person.01230543023.58 schema:affiliation https://www.grid.ac/institutes/grid.419475.a
143 schema:familyName Seidman
144 schema:givenName Michael M.
145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230543023.58
146 rdf:type schema:Person
147 sg:person.01334560047.39 schema:affiliation https://www.grid.ac/institutes/grid.16753.36
148 schema:familyName Thazhathveetil
149 schema:givenName Arun Kalliat
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01334560047.39
151 rdf:type schema:Person
152 sg:pub.10.1038/2530 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016425762
153 https://doi.org/10.1038/2530
154 rdf:type schema:CreativeWork
155 https://app.dimensions.ai/details/publication/pub.1075372021 schema:CreativeWork
156 https://doi.org/10.1002/anie.199306661 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002833413
157 rdf:type schema:CreativeWork
158 https://doi.org/10.1016/s0959-440x(96)80051-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020303860
159 rdf:type schema:CreativeWork
160 https://doi.org/10.1016/s0960-894x(98)00366-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047543664
161 rdf:type schema:CreativeWork
162 https://doi.org/10.1021/bc900008s schema:sameAs https://app.dimensions.ai/details/publication/pub.1055155720
163 rdf:type schema:CreativeWork
164 https://doi.org/10.1021/bi025734y schema:sameAs https://app.dimensions.ai/details/publication/pub.1055195763
165 rdf:type schema:CreativeWork
166 https://doi.org/10.1021/bi035808l schema:sameAs https://app.dimensions.ai/details/publication/pub.1055198049
167 rdf:type schema:CreativeWork
168 https://doi.org/10.1021/bi0520986 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055201296
169 rdf:type schema:CreativeWork
170 https://doi.org/10.1021/bi9816352 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055216419
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1021/ja00010a077 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055698800
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1021/ja01565a074 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055808043
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1073/pnas.88.13.5602 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041380226
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1073/pnas.90.16.7879 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038360632
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1074/jbc.m110.123844 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019782748
181 rdf:type schema:CreativeWork
182 https://doi.org/10.1074/jbc.m800607200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043804600
183 rdf:type schema:CreativeWork
184 https://doi.org/10.1093/nar/12.16.6603 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048404500
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1093/nass/44.1.241 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013687255
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1126/science.1279808 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062471453
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1126/science.271.5250.802 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062552199
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1172/jci19552 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042034032
193 rdf:type schema:CreativeWork
194 https://www.grid.ac/institutes/grid.16753.36 schema:alternateName Northwestern University
195 schema:name Department of Chemistry, Northwestern University, Evanston, IL, USA
196 rdf:type schema:Organization
197 https://www.grid.ac/institutes/grid.419475.a schema:alternateName National Institute on Aging
198 schema:name National Institute on Aging (NIA), NIH, Baltimore, MD, USA
199 rdf:type schema:Organization
200 https://www.grid.ac/institutes/grid.420805.b schema:alternateName Lasergen (United States)
201 schema:name LaserGen, Inc, Houston, TX, USA
202 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...