A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant Embryonic Stem Cells View Full Text


Ontology type: schema:Chapter      Open Access: True


Chapter Info

DATE

2013-07-20

AUTHORS

Hicham Bouabe , Klaus Okkenhaug

ABSTRACT

The completion of human and mouse genome sequencing has confronted us with huge amount of data sequences that certainly need decades and many generations of scientists to be reasonably interpreted and assigned to physiological functions, and subsequently fruitfully translated into medical application. A means to assess the function of genes provides gene targeting in mouse embryonic stem cells (ESCs) that enables to introduce site-specific modifications in the mouse genome, and analyze their physiological consequences. Gene targeting enables almost any type of genetic modifications of interest, ranging from gene insertion (e.g., insertion of human-specific genes or reporter genes), gene disruption, point mutations, and short- and long-range deletions, inversions. Site-specific modification into the genome of ESCs can be reached by homologous recombination using targeting vectors. Here, we describe a protocol to generate targeting constructs and homologous recombinant ESCs. More... »

PAGES

337-354

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-62703-601-6_24

DOI

http://dx.doi.org/10.1007/978-1-62703-601-6_24

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1013409701

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23996269


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