The Comet Assay in Marine Animals View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2013-06-26

AUTHORS

Giada Frenzilli , Brett P. Lyons

ABSTRACT

Comet assay is a quick and versatile technique for assessing DNA damage in individual cells. It allows the detection of DNA single- and double-strand breaks, as well as the presence of alkali-labile sites and cross-links. Here we describe the protocols for the single-cell gel electrophoresis (Comet) assay in its alkaline (pH > 13), mild alkaline (pH = 12.1), and neutral (pH = 8) versions, when applied in marine animals. More... »

PAGES

363-372

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-62703-529-3_19

DOI

http://dx.doi.org/10.1007/978-1-62703-529-3_19

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005483724

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23896887


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aquatic Organisms", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Comet Assay", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA Damage", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Diffusion", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogen-Ion Concentration", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oxidative Stress", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy", 
          "id": "http://www.grid.ac/institutes/grid.5395.a", 
          "name": [
            "Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Frenzilli", 
        "givenName": "Giada", 
        "id": "sg:person.01343570257.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01343570257.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Weymouth Laboratory, CEFAS, Weymouth, Dorset, UK", 
          "id": "http://www.grid.ac/institutes/grid.14332.37", 
          "name": [
            "Weymouth Laboratory, CEFAS, Weymouth, Dorset, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lyons", 
        "givenName": "Brett P.", 
        "id": "sg:person.0626421530.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0626421530.62"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2013-06-26", 
    "datePublishedReg": "2013-06-26", 
    "description": "Comet assay is a quick and versatile technique for assessing DNA damage in individual cells. It allows the detection of DNA single- and double-strand breaks, as well as the presence of alkali-labile sites and cross-links. Here we describe the protocols for the single-cell gel electrophoresis (Comet) assay in its alkaline (pH > 13), mild alkaline (pH = 12.1), and neutral (pH = 8) versions, when applied in marine animals.", 
    "editor": [
      {
        "familyName": "Dhawan", 
        "givenName": "Alok", 
        "type": "Person"
      }, 
      {
        "familyName": "Bajpayee", 
        "givenName": "Mahima", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-62703-529-3_19", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-1-62703-528-6", 
        "978-1-62703-529-3"
      ], 
      "name": "Genotoxicity Assessment", 
      "type": "Book"
    }, 
    "keywords": [
      "marine animals", 
      "double-strand breaks", 
      "single-cell gel electrophoresis", 
      "alkali-labile sites", 
      "DNA damage", 
      "individual cells", 
      "gel electrophoresis", 
      "comet assay", 
      "detection of DNA", 
      "mild alkaline", 
      "DNA", 
      "animals", 
      "electrophoresis", 
      "cells", 
      "assays", 
      "breaks", 
      "alkaline", 
      "sites", 
      "neutral version", 
      "comets", 
      "damage", 
      "versatile technique", 
      "presence", 
      "protocol", 
      "detection", 
      "technique", 
      "version"
    ], 
    "name": "The Comet Assay in Marine Animals", 
    "pagination": "363-372", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1005483724"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-62703-529-3_19"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "23896887"
        ]
      }
    ], 
    "publisher": {
      "name": "Springer Nature", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-62703-529-3_19", 
      "https://app.dimensions.ai/details/publication/pub.1005483724"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2021-11-01T18:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211101/entities/gbq_results/chapter/chapter_324.jsonl", 
    "type": "Chapter", 
    "url": "https://doi.org/10.1007/978-1-62703-529-3_19"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-529-3_19'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-529-3_19'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-529-3_19'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-529-3_19'


 

This table displays all metadata directly associated to this object as RDF triples.

134 TRIPLES      23 PREDICATES      60 URIs      53 LITERALS      15 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-62703-529-3_19 schema:about N1ecc8a2470d04f3f936383b34f69353c
2 N30a5088ec3f545fcabc1d548601d1884
3 N5dd3cdba7dc14fbeab80867db79a97ab
4 N65d09729a7bd4b579c89cf0daad6bdc4
5 N96d32a20d8994de2b6086e913bb4d476
6 Nf7bbd66844ed4a50b7d0a1f9f42bfcc6
7 Nfd6eee19a9124df498186a9ec2cbaebf
8 anzsrc-for:06
9 anzsrc-for:0604
10 schema:author Nf0aa0655da8342d0bc8c8865df12f604
11 schema:datePublished 2013-06-26
12 schema:datePublishedReg 2013-06-26
13 schema:description Comet assay is a quick and versatile technique for assessing DNA damage in individual cells. It allows the detection of DNA single- and double-strand breaks, as well as the presence of alkali-labile sites and cross-links. Here we describe the protocols for the single-cell gel electrophoresis (Comet) assay in its alkaline (pH > 13), mild alkaline (pH = 12.1), and neutral (pH = 8) versions, when applied in marine animals.
14 schema:editor Nff5068db45e94809961b926342ae8a85
15 schema:genre chapter
16 schema:inLanguage en
17 schema:isAccessibleForFree false
18 schema:isPartOf N194f72a45dc8473bb34a2ffdd58fa649
19 schema:keywords DNA
20 DNA damage
21 alkali-labile sites
22 alkaline
23 animals
24 assays
25 breaks
26 cells
27 comet assay
28 comets
29 damage
30 detection
31 detection of DNA
32 double-strand breaks
33 electrophoresis
34 gel electrophoresis
35 individual cells
36 marine animals
37 mild alkaline
38 neutral version
39 presence
40 protocol
41 single-cell gel electrophoresis
42 sites
43 technique
44 versatile technique
45 version
46 schema:name The Comet Assay in Marine Animals
47 schema:pagination 363-372
48 schema:productId N69305bf5d9d54f5a91747665483333d4
49 N84311a9a49144b7eb84cfb5c9a6c0bc5
50 N94e8589d5f184237923aff9306db60af
51 schema:publisher N3c4a0ba85b8a41a88cb69cead463fe9e
52 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005483724
53 https://doi.org/10.1007/978-1-62703-529-3_19
54 schema:sdDatePublished 2021-11-01T18:55
55 schema:sdLicense https://scigraph.springernature.com/explorer/license/
56 schema:sdPublisher N05064dce03104d64a6d2aecdccfb053c
57 schema:url https://doi.org/10.1007/978-1-62703-529-3_19
58 sgo:license sg:explorer/license/
59 sgo:sdDataset chapters
60 rdf:type schema:Chapter
61 N05064dce03104d64a6d2aecdccfb053c schema:name Springer Nature - SN SciGraph project
62 rdf:type schema:Organization
63 N194f72a45dc8473bb34a2ffdd58fa649 schema:isbn 978-1-62703-528-6
64 978-1-62703-529-3
65 schema:name Genotoxicity Assessment
66 rdf:type schema:Book
67 N1ecc8a2470d04f3f936383b34f69353c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
68 schema:name Animals
69 rdf:type schema:DefinedTerm
70 N2a478421211b40f8ac5ba30b48ee7733 schema:familyName Bajpayee
71 schema:givenName Mahima
72 rdf:type schema:Person
73 N30a5088ec3f545fcabc1d548601d1884 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Comet Assay
75 rdf:type schema:DefinedTerm
76 N3bd5424d2f5e480581265ce76f8370b3 rdf:first N2a478421211b40f8ac5ba30b48ee7733
77 rdf:rest rdf:nil
78 N3c4a0ba85b8a41a88cb69cead463fe9e schema:name Springer Nature
79 rdf:type schema:Organisation
80 N5dd3cdba7dc14fbeab80867db79a97ab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Aquatic Organisms
82 rdf:type schema:DefinedTerm
83 N65d09729a7bd4b579c89cf0daad6bdc4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Hydrogen-Ion Concentration
85 rdf:type schema:DefinedTerm
86 N69305bf5d9d54f5a91747665483333d4 schema:name doi
87 schema:value 10.1007/978-1-62703-529-3_19
88 rdf:type schema:PropertyValue
89 N7bf2ce786e424fe78edef302d3479d49 schema:familyName Dhawan
90 schema:givenName Alok
91 rdf:type schema:Person
92 N84311a9a49144b7eb84cfb5c9a6c0bc5 schema:name pubmed_id
93 schema:value 23896887
94 rdf:type schema:PropertyValue
95 N94e8589d5f184237923aff9306db60af schema:name dimensions_id
96 schema:value pub.1005483724
97 rdf:type schema:PropertyValue
98 N96d32a20d8994de2b6086e913bb4d476 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name DNA Damage
100 rdf:type schema:DefinedTerm
101 Nf0aa0655da8342d0bc8c8865df12f604 rdf:first sg:person.01343570257.49
102 rdf:rest Nf0ebf8c0793d4c59b62e2d23886bf1fb
103 Nf0ebf8c0793d4c59b62e2d23886bf1fb rdf:first sg:person.0626421530.62
104 rdf:rest rdf:nil
105 Nf7bbd66844ed4a50b7d0a1f9f42bfcc6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Oxidative Stress
107 rdf:type schema:DefinedTerm
108 Nfd6eee19a9124df498186a9ec2cbaebf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Diffusion
110 rdf:type schema:DefinedTerm
111 Nff5068db45e94809961b926342ae8a85 rdf:first N7bf2ce786e424fe78edef302d3479d49
112 rdf:rest N3bd5424d2f5e480581265ce76f8370b3
113 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
114 schema:name Biological Sciences
115 rdf:type schema:DefinedTerm
116 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
117 schema:name Genetics
118 rdf:type schema:DefinedTerm
119 sg:person.01343570257.49 schema:affiliation grid-institutes:grid.5395.a
120 schema:familyName Frenzilli
121 schema:givenName Giada
122 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01343570257.49
123 rdf:type schema:Person
124 sg:person.0626421530.62 schema:affiliation grid-institutes:grid.14332.37
125 schema:familyName Lyons
126 schema:givenName Brett P.
127 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0626421530.62
128 rdf:type schema:Person
129 grid-institutes:grid.14332.37 schema:alternateName Weymouth Laboratory, CEFAS, Weymouth, Dorset, UK
130 schema:name Weymouth Laboratory, CEFAS, Weymouth, Dorset, UK
131 rdf:type schema:Organization
132 grid-institutes:grid.5395.a schema:alternateName Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy
133 schema:name Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy
134 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...