Methods for Culturing Human Embryonic Stem Cells in a Xeno-Free System View Full Text


Ontology type: schema:Chapter      Open Access: True


Chapter Info

DATE

2013

AUTHORS

Jasmeet Kaur , Mary Lynn Tilkins , Richard Eckert , Shayne Boucher

ABSTRACT

Defined pluripotent stem cell culture media is a valuable tool for tracking and analyzing morphological, cell signaling and gene expression changes in human embryonic stem cells. Cultivation of hESC under xeno-free culture settings provides researchers with a consistent and reproducible environment to test experimental hypotheses and move towards translational and clinical research (Richards et al., Nat Biotechnol 20:933-936, 2002; Richards et al., Stem Cells 21:546-556, 2003). One of the primary concerns of the xenogeneic culture is the transfer of foreign pathogens or antigens that induce disease or immune response by the patient. These undesirable by-products may come from the use of murine-derived feeder cells, xenogeneic matrices, or from animal-derived components found in the cell culture medium or matrix used to isolate or expand the stem cells (Beattie et al., Stem Cells 23:489-495, 2005; Koivisto et al., Reprod Biomed Online 9:330-337, 2004). This chapter describes standardized protocols for obtaining consistent and reproducible results for culturing PSC under xeno-free, feeder-free, or feeder-based systems. More... »

PAGES

115-26

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-62703-348-0_9

DOI

http://dx.doi.org/10.1007/978-1-62703-348-0_9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1008600892

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23546751


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