Directed Differentiation of Pluripotent Stem Cells to Functional Hepatocytes View Full Text


Ontology type: schema:Chapter     


Chapter Info

DATE

2013

AUTHORS

Philip Roelandt , Jolien Vanhove , Catherine Verfaillie

ABSTRACT

Differentiation of human stem cells to hepatocytes is crucial for industrial applications as well as to develop new therapeutic strategies for liver disease. The protocol described here, using sequentially growth factors known to play a role in liver embryonic development, efficiently differentiates human embryonic stem cells (hESC) as well as human-induced pluripotent stem cells (hiPSC) to hepatocytes by directing them through defined embryonic intermediates, namely, mesendoderm/definitive endoderm and hepatoblast and hepatocyte phenotype. After 28 days, the final differentiated progeny is a mixture of cells, comprising cells with characteristics of hepatoblasts and a smaller cell fraction with morphological and phenotypical features of mature hepatocytes. An extensive functional characterization of the stem cell progeny should be used to confirm that differentiated cells display functional characteristics of mature hepatocytes including albumin secretion, glycogen storage, and several detoxifying functions such as urea production, bilirubin conjugation, glutathione S-transferase activity, cytochrome activity and drug transporter activity. More... »

PAGES

141-7

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/978-1-62703-348-0_11

DOI

http://dx.doi.org/10.1007/978-1-62703-348-0_11

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011996516

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23546753


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Culture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Media", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Embryonic Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hepatocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pluripotent Stem Cells", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "KU Leuven", 
          "id": "https://www.grid.ac/institutes/grid.5596.f", 
          "name": [
            "Stem Cell Institute, Katholieke Universiteit Leuven, Leuven, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Roelandt", 
        "givenName": "Philip", 
        "id": "sg:person.01327773553.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327773553.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "KU Leuven", 
          "id": "https://www.grid.ac/institutes/grid.5596.f", 
          "name": [
            "Stem Cell Institute, Katholieke Universiteit Leuven, Leuven, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vanhove", 
        "givenName": "Jolien", 
        "id": "sg:person.0664150051.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664150051.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "KU Leuven", 
          "id": "https://www.grid.ac/institutes/grid.5596.f", 
          "name": [
            "Stem Cell Institute, Katholieke Universiteit Leuven, Leuven, Belgium"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Verfaillie", 
        "givenName": "Catherine", 
        "id": "sg:person.01037646713.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037646713.05"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.jhep.2012.03.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015128236"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0012101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019807585"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth902", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019814710", 
          "https://doi.org/10.1038/nmeth902"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth902", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019814710", 
          "https://doi.org/10.1038/nmeth902"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/hmg/ddh068", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033674653"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076905306", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1080343971", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081860791", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2013", 
    "datePublishedReg": "2013-01-01", 
    "description": "Differentiation of human stem cells to hepatocytes is crucial for industrial applications as well as to develop new therapeutic strategies for liver disease. The protocol described here, using sequentially growth factors known to play a role in liver embryonic development, efficiently differentiates human embryonic stem cells (hESC) as well as human-induced pluripotent stem cells (hiPSC) to hepatocytes by directing them through defined embryonic intermediates, namely, mesendoderm/definitive endoderm and hepatoblast and hepatocyte phenotype. After 28 days, the final differentiated progeny is a mixture of cells, comprising cells with characteristics of hepatoblasts and a smaller cell fraction with morphological and phenotypical features of mature hepatocytes. An extensive functional characterization of the stem cell progeny should be used to confirm that differentiated cells display functional characteristics of mature hepatocytes including albumin secretion, glycogen storage, and several detoxifying functions such as urea production, bilirubin conjugation, glutathione S-transferase activity, cytochrome activity and drug transporter activity.", 
    "editor": [
      {
        "familyName": "Lakshmipathy", 
        "givenName": "Uma", 
        "type": "Person"
      }, 
      {
        "familyName": "Vemuri", 
        "givenName": "Mohan C", 
        "type": "Person"
      }
    ], 
    "genre": "chapter", 
    "id": "sg:pub.10.1007/978-1-62703-348-0_11", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": {
      "isbn": [
        "978-1-62703-347-3", 
        "978-1-62703-348-0"
      ], 
      "name": "Pluripotent Stem Cells", 
      "type": "Book"
    }, 
    "name": "Directed Differentiation of Pluripotent Stem Cells to Functional Hepatocytes", 
    "pagination": "141-7", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/978-1-62703-348-0_11"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "3928743644650f5a50b888180217c1f4130037bad23fcd22ebe39ec95082e364"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1011996516"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "23546753"
        ]
      }
    ], 
    "publisher": {
      "location": "Totowa, NJ", 
      "name": "Humana Press", 
      "type": "Organisation"
    }, 
    "sameAs": [
      "https://doi.org/10.1007/978-1-62703-348-0_11", 
      "https://app.dimensions.ai/details/publication/pub.1011996516"
    ], 
    "sdDataset": "chapters", 
    "sdDatePublished": "2019-04-15T21:00", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8690_00000250.jsonl", 
    "type": "Chapter", 
    "url": "http://link.springer.com/10.1007/978-1-62703-348-0_11"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-348-0_11'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-348-0_11'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-348-0_11'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/978-1-62703-348-0_11'


 

This table displays all metadata directly associated to this object as RDF triples.

139 TRIPLES      23 PREDICATES      43 URIs      29 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/978-1-62703-348-0_11 schema:about N57011470047343c28758d1809bf0bf1a
2 N970409d2b83d4abf9edd6edc8647fa51
3 Naed8a9af621d42f8ae88d1f6e044b608
4 Nc874255a26d24d8bbbe9a56cea5dd6d9
5 Nc9b907657b984c0d98ff3eac8b9944eb
6 Nd0203875d2ed4e00a2e9cd77143251fa
7 Nd2a6184020654ab1968122e093614bb4
8 Nf82f65f0dd2440c0afd5f972cbafa75a
9 anzsrc-for:06
10 anzsrc-for:0601
11 schema:author Na23d9f0aa44a4a36aaa883bd4c29eb28
12 schema:citation sg:pub.10.1038/nmeth902
13 https://app.dimensions.ai/details/publication/pub.1076905306
14 https://app.dimensions.ai/details/publication/pub.1080343971
15 https://app.dimensions.ai/details/publication/pub.1081860791
16 https://doi.org/10.1016/j.jhep.2012.03.030
17 https://doi.org/10.1093/hmg/ddh068
18 https://doi.org/10.1371/journal.pone.0012101
19 schema:datePublished 2013
20 schema:datePublishedReg 2013-01-01
21 schema:description Differentiation of human stem cells to hepatocytes is crucial for industrial applications as well as to develop new therapeutic strategies for liver disease. The protocol described here, using sequentially growth factors known to play a role in liver embryonic development, efficiently differentiates human embryonic stem cells (hESC) as well as human-induced pluripotent stem cells (hiPSC) to hepatocytes by directing them through defined embryonic intermediates, namely, mesendoderm/definitive endoderm and hepatoblast and hepatocyte phenotype. After 28 days, the final differentiated progeny is a mixture of cells, comprising cells with characteristics of hepatoblasts and a smaller cell fraction with morphological and phenotypical features of mature hepatocytes. An extensive functional characterization of the stem cell progeny should be used to confirm that differentiated cells display functional characteristics of mature hepatocytes including albumin secretion, glycogen storage, and several detoxifying functions such as urea production, bilirubin conjugation, glutathione S-transferase activity, cytochrome activity and drug transporter activity.
22 schema:editor N415ad1f67ef9415f8f28a10277d8d9f8
23 schema:genre chapter
24 schema:inLanguage en
25 schema:isAccessibleForFree false
26 schema:isPartOf N2d7ccefa07d8468fba61598dc5eef2f2
27 schema:name Directed Differentiation of Pluripotent Stem Cells to Functional Hepatocytes
28 schema:pagination 141-7
29 schema:productId N10c166c5d06f48f6a3ec31cae719fc8b
30 N3829ba4d8b6d4bb188702e4fc6529073
31 N979c1cff551e47c2b6ec64fe1e89565f
32 N98da734d3f4d4653a916fd6b1417b81d
33 schema:publisher N001dcb53a1c84cd486785b49401fe040
34 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011996516
35 https://doi.org/10.1007/978-1-62703-348-0_11
36 schema:sdDatePublished 2019-04-15T21:00
37 schema:sdLicense https://scigraph.springernature.com/explorer/license/
38 schema:sdPublisher N0298c462f2964170bb6b51210857d16c
39 schema:url http://link.springer.com/10.1007/978-1-62703-348-0_11
40 sgo:license sg:explorer/license/
41 sgo:sdDataset chapters
42 rdf:type schema:Chapter
43 N001dcb53a1c84cd486785b49401fe040 schema:location Totowa, NJ
44 schema:name Humana Press
45 rdf:type schema:Organisation
46 N0298c462f2964170bb6b51210857d16c schema:name Springer Nature - SN SciGraph project
47 rdf:type schema:Organization
48 N10c166c5d06f48f6a3ec31cae719fc8b schema:name readcube_id
49 schema:value 3928743644650f5a50b888180217c1f4130037bad23fcd22ebe39ec95082e364
50 rdf:type schema:PropertyValue
51 N2d7ccefa07d8468fba61598dc5eef2f2 schema:isbn 978-1-62703-347-3
52 978-1-62703-348-0
53 schema:name Pluripotent Stem Cells
54 rdf:type schema:Book
55 N3829ba4d8b6d4bb188702e4fc6529073 schema:name pubmed_id
56 schema:value 23546753
57 rdf:type schema:PropertyValue
58 N415ad1f67ef9415f8f28a10277d8d9f8 rdf:first Nee49227c81934bd3b981b3cea8223d9f
59 rdf:rest Nfad514ca3a2847a9ac88ac5e698c69d5
60 N57011470047343c28758d1809bf0bf1a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
61 schema:name Hepatocytes
62 rdf:type schema:DefinedTerm
63 N970409d2b83d4abf9edd6edc8647fa51 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
64 schema:name Humans
65 rdf:type schema:DefinedTerm
66 N979c1cff551e47c2b6ec64fe1e89565f schema:name dimensions_id
67 schema:value pub.1011996516
68 rdf:type schema:PropertyValue
69 N98da734d3f4d4653a916fd6b1417b81d schema:name doi
70 schema:value 10.1007/978-1-62703-348-0_11
71 rdf:type schema:PropertyValue
72 Na23d9f0aa44a4a36aaa883bd4c29eb28 rdf:first sg:person.01327773553.26
73 rdf:rest Nc80a81909db8405abf44dcf731e27316
74 Na941c7c0d67c413c891193fd1e10a96e schema:familyName Vemuri
75 schema:givenName Mohan C
76 rdf:type schema:Person
77 Naed8a9af621d42f8ae88d1f6e044b608 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
78 schema:name Cells, Cultured
79 rdf:type schema:DefinedTerm
80 Nc80a81909db8405abf44dcf731e27316 rdf:first sg:person.0664150051.72
81 rdf:rest Nd0fa7a03dd144dac9dc799ac54807b2c
82 Nc874255a26d24d8bbbe9a56cea5dd6d9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Embryonic Stem Cells
84 rdf:type schema:DefinedTerm
85 Nc9b907657b984c0d98ff3eac8b9944eb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Cell Differentiation
87 rdf:type schema:DefinedTerm
88 Nd0203875d2ed4e00a2e9cd77143251fa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Cell Culture Techniques
90 rdf:type schema:DefinedTerm
91 Nd0fa7a03dd144dac9dc799ac54807b2c rdf:first sg:person.01037646713.05
92 rdf:rest rdf:nil
93 Nd2a6184020654ab1968122e093614bb4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Culture Media
95 rdf:type schema:DefinedTerm
96 Nee49227c81934bd3b981b3cea8223d9f schema:familyName Lakshmipathy
97 schema:givenName Uma
98 rdf:type schema:Person
99 Nf82f65f0dd2440c0afd5f972cbafa75a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Pluripotent Stem Cells
101 rdf:type schema:DefinedTerm
102 Nfad514ca3a2847a9ac88ac5e698c69d5 rdf:first Na941c7c0d67c413c891193fd1e10a96e
103 rdf:rest rdf:nil
104 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
105 schema:name Biological Sciences
106 rdf:type schema:DefinedTerm
107 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
108 schema:name Biochemistry and Cell Biology
109 rdf:type schema:DefinedTerm
110 sg:person.01037646713.05 schema:affiliation https://www.grid.ac/institutes/grid.5596.f
111 schema:familyName Verfaillie
112 schema:givenName Catherine
113 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037646713.05
114 rdf:type schema:Person
115 sg:person.01327773553.26 schema:affiliation https://www.grid.ac/institutes/grid.5596.f
116 schema:familyName Roelandt
117 schema:givenName Philip
118 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327773553.26
119 rdf:type schema:Person
120 sg:person.0664150051.72 schema:affiliation https://www.grid.ac/institutes/grid.5596.f
121 schema:familyName Vanhove
122 schema:givenName Jolien
123 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664150051.72
124 rdf:type schema:Person
125 sg:pub.10.1038/nmeth902 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019814710
126 https://doi.org/10.1038/nmeth902
127 rdf:type schema:CreativeWork
128 https://app.dimensions.ai/details/publication/pub.1076905306 schema:CreativeWork
129 https://app.dimensions.ai/details/publication/pub.1080343971 schema:CreativeWork
130 https://app.dimensions.ai/details/publication/pub.1081860791 schema:CreativeWork
131 https://doi.org/10.1016/j.jhep.2012.03.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015128236
132 rdf:type schema:CreativeWork
133 https://doi.org/10.1093/hmg/ddh068 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033674653
134 rdf:type schema:CreativeWork
135 https://doi.org/10.1371/journal.pone.0012101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019807585
136 rdf:type schema:CreativeWork
137 https://www.grid.ac/institutes/grid.5596.f schema:alternateName KU Leuven
138 schema:name Stem Cell Institute, Katholieke Universiteit Leuven, Leuven, Belgium
139 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...